Linkage map from F₂ population of Zong128 × KuchuT94-4.

<p>Linkage groups that could not be assigned to a chromosome were given the preliminary names UnLG1 UnLG2 and UnLG3. Bars and lines on the right-hand side of the linkage groups show the QTL likelihood intervals. Map distances in centimorgans (cM) are indicated on the left-hand side of the linkage groups. LC, lint color; FC, fuzz color; FL, fiber length; FU, fiber uniformity; FF, micronaire; FE, fiber elongation; FS, fiber strength; *Indicates distorted markers.</p

Additional file 4: Figure S4. of Single-base resolution methylomes of upland cotton (Gossypium hirsutum L.) reveal epigenome modifications in response to drought stress

DMRs length analysis in each chromosome between drought and control sample (PNG 107 kb

Linkage map from RIL population of Zong128 × KuchuT94-4.

<p>Linkage groups that could not be assigned to a chromosome were given the preliminary names UnLG1 –UnLG5. Bars and lines on the right-hand side of the linkage groups show the QTL likelihood intervals. Map distances in centimorgans (cM) are indicated on the left-hand side of the linkage groups. LC, lint color; FC, fuzz color; FL, fiber length; FU, fiber uniformity; FF, micronaire; FE, fiber elongation; FS, fiber strength; *Indicates distorted markers.</p

Linkage map from F₂ of Zong128 × Liao96-23-30 population.

<p>Linkage groups that could not be assigned to a chromosome were given the preliminary names UnLG1. Bars and lines on the right-hand side of the linkage groups show the QTL likelihood intervals. Map distances in centimorgans (cM) are indicated on the left-hand side of the linkage groups. LC, lint color; FC, fuzz color; FL, fiber length; FU, fiber uniformity; FF, micronaire; FE, fiber elongation; FS, fiber strength; *Indicates distorted markers.</p

Expression levels of stress-responsive genes (<i>RD29A</i>, <i>RD29B</i>, <i>SOS2</i> and <i>CBL1</i>) in control plants and transgenic lines.

<p>Columns marked with different lower case letters indicate a significant difference (p < 0.05) from the WT treatment.</p

The phylogenetic relationship between GhSOS1 and SOS1 from other plant species.

<p>The phylogenetic tree was constructed using MEGA ver. 5.0. The following protein sequences were used to construct of the phylogenetic tree: <i>Arabidopsis thaliana</i> AtSOS1 (AF256224) and AtNHX1 (AF510074), <i>Chrysanthemum crassum</i> CcSOS1 (AB439132), <i>Halostachys caspica</i> HcNHX1 (GU188850), <i>Helianthus tuberosus</i> HtSOS1 (KC410809), <i>Kosteletzkya virginica</i> KvSOS1 (KJ577576), <i>Limonium gmelinii</i> LgSOS1 (EU780458), <i>Oryza sativa</i> OsSOS1 (AY785147) and OsNHX1 (AB021878), <i>Populus euphratica</i> PeSOS1 (DQ517530) and PeNHX1 (FJ866610), <i>Pennisetum glaucum</i> PgNHX1 (DQ071264), <i>Salicornia brachiata</i> SbSOS1 (EU879059), <i>Salicornia europaea</i> SeNHX1 (AY131235), <i>Suaeda japonica</i> SjSOS1 (AB198179), <i>Suaeda salsa</i> SsSOS1 (KF914414), <i>Theobroma cacao</i> TcSOS1 (XM_007045345) and TcNHX1 (XM_007030729), <i>Thellungiella halophila</i> ThSOS1 (EF207775), <i>Vitis vinifera</i> VvSOS1 (CAO42437) and <i>Zoysia japonica</i> ZjNHX1 (EU333827).</p

A. the lint colored of parents and F₁ generation; B: fuzz color of parents and F₁ generation.

<p>A. the lint colored of parents and F₁ generation; B: fuzz color of parents and F₁ generation.</p

Additional file 4: Figure S4. of Single-base resolution methylomes of upland cotton (Gossypium hirsutum L.) reveal epigenome modifications in response to drought stress

DMRs length analysis in each chromosome between drought and control sample (PNG 107 kb

Sodium and potassium content in <i>GhSOS1</i>-overexpressing and control plants.

<p>(A) Na⁺ content; (B) K⁺ content; (C) Na⁺/K⁺ ration. (WT indicates wild-type <i>Arabidopsis</i>). Columns marked with different lower case letters indicate a significant difference (p < 0.05) from the WT treatment.</p

Salt tolerance of <i>GhSOS1</i> transgenic <i>Arabidopsis</i> plants.

<p>(A) <i>GhSOS1</i> expression in wild type (WT) and transgenic lines (L12 and L14). (B) Responses of transgenic and WT <i>Arabidopsis</i> plants grown in pots under normal conditions and salt stress. (C) MDA content of WT and transgenic plants with or without salt stress treatments. Columns marked with different lower case letters indicate a significant difference (p < 0.05) from the WT treatment.</p