Isotopic analysis

δ15N of environmental nitrate and ammonia, as well as long-skinny and short-fat Ridgeia tubeworm

Reference gene evaluation

Best reference gene: evaluated by NormFinder and GeNor

Nitrate and ammonia concentrations

Concentrations of nitrate and ammonia determined in Ridgeia bloo

DNA sequences amplified by qPCR primers

DNA sequences were generated by sanger sequencing of amplicons from qPCR primer