Numerical analysis of underwater flow past columnar projectile with different cross-sections at high Reynolds numbers

Based on Detached Eddy Simulation (DES) technique, the flow around a columnar projectile with different cross-section shapes in the supercritical and extremely supercritical region is simulated by the Fluent. The cross-section of the projectile is regular polygon, which number of edges is 4, 6, 8, 10, 12, 24 and ∞, where ∞ means a circle. The vortex shedding pattern and flow field characteristics are analyzed at Reynolds number 2.5×105 to 2×107. Regarding circular cylinder projectile, when the flow velocity changes from 25 m/s to 200 m/s, the average drag coefficient decreases, and the St Number increases. Regarding regular polygon, when the number of edges for polygon changes from 4 to ∞ at flow velocity 50 m/s, the average drag coefficient decreases, and the St Number increases. The average lift coefficient is almost equal to zero and does not change with the flow velocity and the cross-section. The pressure coefficient Cp of 4-prism, 6-prism, 8-prism, 12-prism and 24-prism has multiple local minimum values at the polygon vertices of the cross section. According to the spectrum analysis, the vortex shedding frequency of 4-prism, 24-prism and cylindrical is single and fixed, so the projectile may cause resonance and deviates from a predetermined trajectory. But for the 6-prism and 8-prism and 12-prism, the cl and cd is multi-periodic vibration. So, considering the flow induced structural vibrations, drag, the power of shrapnel and manufacturing cost, the 8-prism are better choices for cluster warhead underwater in engineering design

Numerical analysis on aerodynamic characteristics of short cylindrical terminal-sensitive bullet

The flow-induced lateral vibration phenomenon of the terminal sensitive bullets (TSB) when it is dispersed by the airborne distributor is taken as the research background. Based on the Fluent, the flow around a rotating short cylindrical TSB (L/D < 1) is simulated and analyzed varying with relative rotation velocity at high Re number (1×105 ≤ Re ≤ 3×106). The simulation results show that the flow field structure of the short cylinder with two free ends is different from the symmetry of the short cylindrical flow field with one free end, and there is no horseshoe vortex. Compared to the long cylinder with double free ends, the Cd of the short cylinder is more sensitive to the change of Re. With the increase of Re, the Cd of the short cylinder decreases, and the value is between the infinite cylinder and the sphere in the non-critical region. When the short cylinder rotates at the angular velocity ω, the top vortex bends and deforms to a ‘C’ shape on the leeward side where the fluid is accelerated. Due to the periodic disturbance of the detector, the aerodynamic coefficient of the rotating TSB is periodically vibrated. In a single cycle, the waveform of the cd shows a ‘W’ shape, and the waveform of the cl is ‘M’

Bis{2,4-dibromo-6-[(2-phenyl­eth­yl)imino­meth­yl]phenolato-κ2 N,O}cobalt(II)

In the title complex, [Co(C15H12Br2NO)2], the CoII atom is four-coordinated by two N,O-bidentate chelate Schiff base ligands, displaying a flattened tetra­hedral coordination environment. The CoII atom occupies a special position on a twofold rotation axis. In the crystal, mol­ecules are linked via weak C—H⋯Br inter­actions

Characterization of plasma thiol redox potential in a common marmoset model of aging☆

Due to its short lifespan, ease of use and age-related pathologies that mirror those observed in humans, the common marmoset (Callithrix jacchus) is poised to become a standard nonhuman primate model of aging. Blood and extracellular fluid possess two major thiol-dependent redox nodes involving cysteine (Cys), cystine (CySS), glutathione (GSH) and glutathione disulfide (GSSG). Alteration in these plasma redox nodes significantly affects cellular physiology, and oxidation of the plasma Cys/CySS redox potential (EhCySS) is associated with aging and disease risk in humans. The purpose of this study was to determine age-related changes in plasma redox metabolites and corresponding redox potentials (Eh) to further validate the marmoset as a nonhuman primate model of aging. We measured plasma thiol redox states in marmosets and used existing human data with multivariate adaptive regression splines (MARS) to model the relationships between age and redox metabolites. A classification accuracy of 70.2% and an AUC of 0.703 were achieved using the MARS model built from the marmoset redox data to classify the human samples as young or old. These results show that common marmosets provide a useful model for thiol redox biology of aging

Metabolic Characterization of the Common Marmoset (Callithrix jacchus)

High-resolution metabolomics has created opportunity to integrate nutrition and metabolism into genetic studies to improve understanding of the diverse radiation of primate species. At present, however, there is very little information to help guide experimental design for study of wild populations. In a previous non-targeted metabolomics study of common marmosets (Callithrix jacchus), Rhesus macaques, humans, and four non-primate mammalian species, we found that essential amino acids (AA) and other central metabolites had interspecies variation similar to intraspecies variation while non-essential AA, environmental chemicals and catabolic waste products had greater interspecies variation. The present study was designed to test whether 55 plasma metabolites, including both nutritionally essential and non-essential metabolites and catabolic products, differ in concentration in common marmosets and humans. Significant differences were present for more than half of the metabolites analyzed and included AA, vitamins and central lipid metabolites, as well as for catabolic products of AA, nucleotides, energy metabolism and heme. Three environmental chemicals were present at low nanomolar concentrations but did not differ between species. Sex and age differences in marmosets were present for AA and nucleotide metabolism and warrant additional study. Overall, the results suggest that quantitative, targeted metabolomics can provide a useful complement to non-targeted metabolomics for studies of diet and environment interactions in primate evolution.National Institutes of Health (U.S.) (grant AG038746

Numerical analysis of underwater flow past columnar projectile with different cross-sections at high Reynolds numbers

Based on Detached Eddy Simulation (DES) technique, the flow around a columnar projectile with different cross-section shapes in the supercritical and extremely supercritical region is simulated by the Fluent. The cross-section of the projectile is regular polygon, which number of edges is 4, 6, 8, 10, 12, 24 and ∞, where ∞ means a circle. The vortex shedding pattern and flow field characteristics are analyzed at Reynolds number 2.5×105 to 2×107. Regarding circular cylinder projectile, when the flow velocity changes from 25 m/s to 200 m/s, the average drag coefficient decreases, and the St Number increases. Regarding regular polygon, when the number of edges for polygon changes from 4 to ∞ at flow velocity 50 m/s, the average drag coefficient decreases, and the St Number increases. The average lift coefficient is almost equal to zero and does not change with the flow velocity and the cross-section. The pressure coefficient Cp of 4-prism, 6-prism, 8-prism, 12-prism and 24-prism has multiple local minimum values at the polygon vertices of the cross section. According to the spectrum analysis, the vortex shedding frequency of 4-prism, 24-prism and cylindrical is single and fixed, so the projectile may cause resonance and deviates from a predetermined trajectory. But for the 6-prism and 8-prism and 12-prism, the cl and cd is multi-periodic vibration. So, considering the flow induced structural vibrations, drag, the power of shrapnel and manufacturing cost, the 8-prism are better choices for cluster warhead underwater in engineering design

Genome-wide investigation and functional characterization of the β-ketoadipate pathway in the nitrogen-fixing and root-associated bacterium Pseudomonas stutzeri A1501

<p>Abstract</p> <p>Background</p> <p>Soil microorganisms are mainly responsible for the complete mineralization of aromatic compounds that usually originate from plant products or environmental pollutants. In many cases, structurally diverse aromatic compounds can be converted to a small number of structurally simpler intermediates, which are metabolized to tricarboxylic acid intermediates via the β-ketoadipate pathway. This strategy provides great metabolic flexibility and contributes to increased adaptation of bacteria to their environment. However, little is known about the evolution and regulation of the β-ketoadipate pathway in root-associated diazotrophs.</p> <p>Results</p> <p>In this report, we performed a genome-wide analysis of the benzoate and 4-hydroxybenzoate catabolic pathways of <it>Pseudomonas stutzeri </it>A1501, with a focus on the functional characterization of the β-ketoadipate pathway. The <it>P. stutzeri </it>A1501 genome contains sets of catabolic genes involved in the peripheral pathways for catabolism of benzoate (<it>ben</it>) and 4-hydroxybenzoate (<it>pob</it>), and in the catechol (<it>cat</it>) and protocatechuate (<it>pca</it>) branches of the β-ketoadipate pathway. A particular feature of the catabolic gene organization in A1501 is the absence of the <it>catR </it>and <it>pcaK </it>genes encoding a LysR family regulator and 4-hydroxybenzoate permease, respectively. Furthermore, the BenR protein functions as a transcriptional activator of the <it>ben </it>operon, while transcription from the <it>catBC </it>promoter can be activated in response to benzoate. Benzoate degradation is subject to carbon catabolite repression induced by glucose and acetate in A1501. The HPLC analysis of intracellular metabolites indicated that low concentrations of 4-hydroxybenzoate significantly enhance the ability of A1501 to degrade benzoate.</p> <p>Conclusions</p> <p>The expression of genes encoding proteins involved in the β-ketoadipate pathway is tightly modulated by both pathway-specific and catabolite repression controls in A1501. This strain provides an ideal model system for further study of the evolution and regulation of aromatic catabolic pathways.</p

Identification of newly developed advanced schistosomiasis with MALDI-TOF mass spectrometry and ClinProTools analysis

Cases of newly developed advanced schistosomiasis (NDAS) have occurred in areas where schistosomiasis transmission has been blocked for more than 25 years. The causes and pathogenesis of NDAS are still unknown. Diagnosis of NDAS relies on historical investigation and clinical symptoms, such as liver fibrosis, hepatic ascites and abnormal biochemical indexes in serum. It is important but difficult at this stage to develop a new tool for early screening and rapid diagnosis. In this study, serum peptides from thirty patients with NDAS and thirty healthy controls were captured with weak cation exchange magnetic beads, and subjected to MALDI-TOF mass spectrometry and ClinProTools analysis. Eleven peaks with m/z 924, 2661, 2953, 2991, 3241, 3884, 5337, 5905, 5943, 7766 and 9289 were decreased and three peaks with m/z 1945, 2082 and 4282 were increased in the NDAS group. The proteomic detection pattern (PDP) was established with 14 different peptide peaks, and its sensitivity and specificity were investigated with a blind test. The peptide mass fingerprints of sera from 50 NDAS patients and 100 healthy controls were double-blind subjected to the PDP method, and 50 patients and 92 healthy controls were classified as NDAS and healthy separately, which showed 100% sensitivity and 92% specificity. Our results showed that the PDP could be a new and useful method to detect NDAS