LE2Fusion: A novel local edge enhancement module for infrared and visible image fusion

Infrared and visible image fusion task aims to generate a fused image which contains salient features and rich texture details from multi-source images. However, under complex illumination conditions, few algorithms pay attention to the edge information of local regions which is crucial for downstream tasks. To this end, we propose a fusion network based on the local edge enhancement, named LE2Fusion. Specifically, a local edge enhancement (LE2) module is proposed to improve the edge information under complex illumination conditions and preserve the essential features of image. For feature extraction, a multi-scale residual attention (MRA) module is applied to extract rich features. Then, with LE2, a set of enhancement weights are generated which are utilized in feature fusion strategy and used to guide the image reconstruction. To better preserve the local detail information and structure information, the pixel intensity loss function based on the local region is also presented. The experiments demonstrate that the proposed method exhibits better fusion performance than the state-of-the-art fusion methods on public datasets

WavePF: A Novel Fusion Approach based on Wavelet-guided Pooling for Infrared and Visible Images

Infrared and visible image fusion aims to generate synthetic images simultaneously containing salient features and rich texture details, which can be used to boost downstream tasks. However, existing fusion methods are suffering from the issues of texture loss and edge information deficiency, which result in suboptimal fusion results. Meanwhile, the straight-forward up-sampling operator can not well preserve the source information from multi-scale features. To address these issues, a novel fusion network based on the wavelet-guided pooling (wave-pooling) manner is proposed, termed as WavePF. Specifically, a wave-pooling based encoder is designed to extract multi-scale image and detail features of source images at the same time. In addition, the spatial attention model is used to aggregate these salient features. After that, the fused features will be reconstructed by the decoder, in which the up-sampling operator is replaced by the wave-pooling reversed operation. Different from the common max-pooling technique, image features after the wave-pooling layer can retain abundant details information, which can benefit the fusion process. In this case, rich texture details and multi-scale information can be maintained during the reconstruction phase. The experimental results demonstrate that our method exhibits superior fusion performance over the state-of-the-arts on multiple image fusion benchmark

Identification and Biosynthetic Study of the Siderophore Lysochelin in the Biocontrol Agent Lysobacter enzymogenes

Lysobacter is a genus of bacteria emerging as new biocontrol agents in agriculture. Although iron acquisition is essential for the bacteria, no siderophore has been identified from any Lysobacter. Here, we report the identification of the first siderophore, N1,N8-bis(2,3-dihydroxybenzoyl)spermidine (lysochelin), and its biosynthetic gene cluster from Lysobacter enzymogenes. Intriguingly, the deletion of the spermidine biosynthetic gene encoding arginine decarboxylase or SAM decarboxylase eliminated lysochelin and the antifungals, HSAF and its analogues, which are key to the disease control activity and to the survival of Lysobacter under oxidative stresses caused by excess iron. The production of lysochelin and the antifungals is greatly affected by iron concentration. Together, the results revealed a previously unrecognized system, in which L. enzymogenes produces a group of small molecules, lysochelin, spermidine, and HSAF and its analogues, that are affected by iron concentration and critical to the growth and survival of the biocontrol agent

Characterizing current noise of commercial constant-current sources by using of an optically-pumped rubidium atomic magnetometer

This paper introduces a method for characterizing the current noise of commercial constant-current sources(CCSs) using a free-induction-decay(FID) type optically-pumped rubidium atomic magnetometer driven by a radio-frequency(RF) magnetic field. We convert the sensitivity of the atomic magnetometer into the current noise of CCS by calibrating the coil constant. At the same time, the current noise characteristics of six typical commercial low-noise CCSs are compared. The current noise level of the KeySight Model B2961A is the lowest among the six tested CCSs, which is 36.233 0.022 nA / Hz1/2 at 1-25 Hz and 133.905 0.080 nA / Hz1/2 at 1-100 Hz respectively. The sensitivity of atomic magnetometer is dependent on the current noise level of the CCS. The CCS with low noise is of great significance for high-sensitivity atomic magnetometer. The research provides an important reference for promoting the development of high precision CCS, metrology and basic physics research.Comment: 7pages,7figure

Blockade of Treg derived TGF-β abrogates suppression of effector T cell function within the tumor microenvironment

Regulatory T cells (Treg) play a role in suppression of anti-melanoma immunity; however, the exact mechanism is poorly understood. Through intravital two photon microscopy, we found that Pmel-1 effectors engage in cell-cell interactions with tumor resident Tregs. To determine if contact between Tregs and T effectors (Teff) hinders killing of tumor cells in vivo, we utilized ex-vivo three-dimensional collagen-fibrin gel cultures of B16 melanoma cells. Collagen-fibrin gel cultures recapitulated the in vivo suppression, rendering the dissociated tumor resistant to killing by in vitro activated antigen specific Teff. In vivo depletion of Tregs in foxp3-DTR mice prior to tumor excision reversed the suppression. Additionally, In vivo modulation of intra-tumor Tregs suppressive function by GITR ligation had a similar effect, leading to ex-vivo tumor killing. Using neutralizing antibodies, we found that blocking TGF-β reversed the suppression. In addition, soluble factors from collagen-fibrin gel tumors do not inhibit killing suggesting that suppression is contact or proximity dependent. The CD8 Teff recovered from these gels exhibit a decrease in Granzyme B expression and an increase in expression of T cell exhaustion marker PD-1. These findings support the conclusion that intra-tumor contact with Tregs during the effector phase of the immune response is responsible for inhibiting anti-melanoma immunity in a TGF-β dependent manner, elucidating a novel way to target intratumoral Tregs

Genome-scale analysis and comparison of gene expression profiles in developing and germinated pollen in Oryza sativa

<p>Abstract</p> <p>Background</p> <p>Pollen development from the microspore involves a series of coordinated cellular events, and the resulting mature pollen has a specialized function to quickly germinate, produce a polar-growth pollen tube derived from the vegetative cell, and deliver two sperm cells into the embryo sac for double fertilization. The gene expression profiles of developing and germinated pollen have been characterised by use of the eudicot model plant <it>Arabidopsis</it>. Rice, one of the most important cereal crops, has been used as an excellent monocot model. A comprehensive analysis of transcriptome profiles of developing and germinated pollen in rice is important to understand the conserved and diverse mechanism underlying pollen development and germination in eudicots and monocots.</p> <p>Results</p> <p>We used Affymetrix GeneChip^®Rice Genome Array to comprehensively analyzed the dynamic changes in the transcriptomes of rice pollen at five sequential developmental stages from microspores to germinated pollen. Among the 51,279 transcripts on the array, we found 25,062 pollen-preferential transcripts, among which 2,203 were development stage-enriched. The diversity of transcripts decreased greatly from microspores to mature and germinated pollen, whereas the number of stage-enriched transcripts displayed a "U-type" change, with the lowest at the bicellular pollen stage; and a transition of overrepresented stage-enriched transcript groups associated with different functional categories, which indicates a shift in gene expression program at the bicellular pollen stage. About 54% of the now-annotated rice F-box protein genes were expressed preferentially in pollen. The transcriptome profile of germinated pollen was significantly and positively correlated with that of mature pollen. Analysis of expression profiles and coexpressed features of the pollen-preferential transcripts related to cell cycle, transcription, the ubiquitin/26S proteasome system, phytohormone signalling, the kinase system and defense/stress response revealed five expression patterns, which are compatible with changes in major cellular events during pollen development and germination. A comparison of pollen transcriptomes between rice and <it>Arabidopsis </it>revealed that 56.6% of the rice pollen preferential genes had homologs in <it>Arabidopsis </it>genome, but 63.4% of these homologs were expressed, with a small proportion being expressed preferentially, in <it>Arabidopsis </it>pollen. Rice and <it>Arabidopsis </it>pollen had non-conservative transcription factors each.</p> <p>Conclusions</p> <p>Our results demonstrated that rice pollen expressed a set of reduced but specific transcripts in comparison with vegetative tissues, and the number of stage-enriched transcripts displayed a "U-type" change during pollen development, with the lowest at the bicellular pollen stage. These features are conserved in rice and <it>Arabidopsis</it>. The shift in gene expression program at the bicellular pollen stage may be important to the transition from earlier cell division to later pollen maturity. Pollen at maturity pre-synthesized transcripts needed for germination and early pollen tube growth. The transcription regulation associated with pollen development would have divergence between the two species. Our results also provide novel insights into the molecular program and key components of the regulatory network regulating pollen development and germination.</p

An extensive anoxic event in the Triassic of the South China Block : a pyrite framboid study form Dajiang and its implications for the cause(s) of oxygen depletion

Water column oxygen deficiency has been considered as a potent driver of the extinction of marine benthos, and is a main feature of marine environments in the aftermath of the end-Permian mass extinction. The record of Permian-Triassic anoxia is more complex than previously thought, and is seen to vary between different palaeogeographic settings, but a full understanding is hindered by a paucity of evidence. During the Permian-Triassic interval the South China Block was located equatorially with Palaeotethys to the north and western Panthalassa to the south. This specific configuration provides a unique opportunity to compare the extent and duration of oxygen deficiency in Palaeotethys and Panthalassa under broadly similar climatic conditions. Sedimentary facies and pyrite framboid size-frequency distributions suggest that the oxygen-poor conditions became widespread across the shallow-marine carbonate platform of the South China Block immediately above the Permian-Triassic boundary and mass extinction level. Oxygen deficiency was most intense at the southern margin of the block where it met Panthalassa. Proposed drivers of the expansion of oxygen minimum zones into platform settings include enhanced terrigenous input and/or ocean stratification, or alternatively the upwelling of nutrient-rich deep ocean water. The former mechanisms are theoretically more likely to have operated in the relatively restricted Palaeotethys which was surrounded by ancient lands. In contrast, Panthalassa would likely have experienced stronger oceanic circulation and therefore be more susceptible to the effects of upwelling. Although variations in the record of the South China Block anoxic event might reflect local factors, the greater intensity of oxygen deficiency and a concomitant larger negative shift in carbonate carbon isotopes on its Panthalassan margin point to a key role for upwelling. This mechanism was likely a major driver of the Permian-Triassic global oceanic anoxic event, which itself was at least partly responsible for the ongoing inhospitable conditions and delayed recovery following the end-Permian extinction

Evolution of flower color pattern through selection on regulatory small RNAs

Small RNAs (sRNAs) regulate genes in plants and animals. Here, we show that population-wide differences in color patterns in snapdragon flowers are caused by an inverted duplication that generates sRNAs. The complexity and size of the transcripts indicate that the duplication represents an intermediate on the pathway to microRNA evolution. The sRNAs repress a pigment biosynthesis gene, creating a yellow highlight at the site of pollinator entry. The inverted duplication exhibits steep clines in allele frequency in a natural hybrid zone, showing that the allele is under selection. Thus, regulatory interactions of evolutionarily recent sRNAs can be acted upon by selection and contribute to the evolution of phenotypic diversity

The application and research progress of anti-angiogenesis therapy in tumor immunotherapy

Tumor immunotherapy, as the focus of scientific research and clinical tumor treatment in recent years, has received extensive attention. Due to its remarkable curative effect and fewer side effects than traditional treatments, it has significant clinical benefits for the treatment of various advanced cancers and can improve cancer patient survival in the long term. Currently, most patients cannot benefit from immunotherapy, and some patients may experience tumor recurrence and drug resistance even if they achieve remission overcome. Numerous studies have shown that the abnormal angiogenesis state of tumors can lead to immunosuppressive tumor microenvironment, which affects the efficacy of immunotherapy. Actually, to improve the efficacy of immunotherapy, the application of anti-angiogenesis drugs to normalize abnormal tumor vessel has been widely confirmed in basic and clinical research. This review not only discusses the risk factors, mechanisms, and effects of abnormal and normalized tumor angiogenesis state on the immune environment, but summarizes the latest progress of immunotherapy combined with anti-angiogenic therapy. We hope this review provides an applied reference for anti-angiogenesis drugs and synergistic immunotherapy therapy