279 research outputs found
Impulse for animal welfare outside the experiment
Animal welfare is a growing societal concern and the well-being of animals used for experimental purposes is under particular scrutiny. The vast majority of laboratory animals are mice living in small cages that do not offer very much variety. Moreover, the experimental procedure often takes very little time compared to the time these animals have been bred to the desired age or are being held available for animal experimentation. However, for the assessment of animal welfare, the time spent waiting for an experiment or the time spent after finishing an experiment has also to be taken into account. In addition to experimental animals, many additional animals (e.g. for breeding and maintenance of genetic lines, surplus animals) are related to animal experimentation and usually face similar living conditions. Therefore, in terms of improving the overall welfare of laboratory animals, there is not only a need for refinement of experimental conditions but especially for improving living conditions outside the experiment. The improvement of animal welfare thus depends to a large extent on the housing and maintenance conditions of all animals related to experimentation. Given the current state of animal welfare research there is indeed a great potential for improving the overall welfare of laboratory animals
Do we need a basis bolus concept for sedoanalgesia of mechanically ventilated patients in ICU?
Current Methods to Investigate Nociception and Pain in Zebrafish
Pain is an unpleasant, negative emotion and its debilitating effects are complex to manage. Mammalian models have long dominated research on nociception and pain, but there is increasing evidence for comparable processes in fish. The need to improve existing pain models for drug research and the obligation for 3R refinement of fish procedures facilitated the development of numerous new assays of nociception and pain in fish. The zebrafish is already a well-established animal model in many other research areas like toxicity testing, as model for diseases or regeneration and has great potential in pain research, too. Methods of electrophysiology, molecular biology, analysis of reflexive or non-reflexive behavior and fluorescent imaging are routinely applied but it is the combination of these tools what makes the zebrafish model so powerful. Simultaneously, observing complex behavior in free-swimming larvae, as well as their neuronal activity at the cellular level, opens new avenues for pain research. This review aims to supply a toolbox for researchers by summarizing current methods to study nociception and pain in zebrafish. We identify treatments with the best algogenic potential, be it chemical, thermal or electric stimuli and discuss options of analgesia to counter effects of nociception and pain by opioids, non-steroidal anti-inflammatory drugs (NSAIDs) or local anesthetics. In addition, we critically evaluate these practices, identify gaps of knowledge and outline potential future developments
Lifetime development of behavioural phenotype in the house mouse (Mus musculus)
Brust V, Schindler PM, Lewejohann L. Lifetime development of behavioural phenotype in the house mouse (Mus musculus). Frontiers in Zoology. 2015;12(Suppl. 1): S17.With each trajectory taken during the ontogeny of an individual, the number of optional behavioural phenotypes
that can be expressed across its life span is reduced. The initial range of phenotypic plasticity is largely determined
by the genetic material/composition of the gametes whereas interacting with the given environment shapes
individuals to adapt to/cope with specific demands. In mammalian species, the phenotype is shaped as the foetus
grows, depending on the environment in the uterus, which in turn depends on the outer environment the mother
experiences during pregnancy. After birth, a complex interaction between innate constitution and environmental
conditions shapes individual lifetime trajectories, bringing about a wide range of diversity among individual
subjects.
In laboratory mice inbreeding has been systematically induced in order to reduce the genetic variability between
experimental subjects. In addition, within most laboratories conducting behavioural phenotyping with mice,
breeding and housing conditions are highly standardised. Despite such standardisation efforts a considerable
amount of variability persists in the behaviour of mice. There is good evidence that phenotypic variation is not
merely random but might involve individual specific behavioural patterns consistent over time. In order to
understand the mechanisms and the possible adaptive value of the maintenance of individuality we review the
emergence of behavioural phenotypes over the course of the life of (laboratory) mice. We present a literature
review summarizing developmental stages of behavioural development of mice along with three illustrative case
studies. We conclude that the accumulation of environmental differences and experiences lead to a
“mouse individuality” that becomes increasingly stable over the lifetime
Impulse for animal welfare outside the experiment
Animal welfare is a growing societal concern and the well-being of animals used for experimental purposes is under particular scrutiny. The vast majority of laboratory animals are mice living in small cages that do not offer very much variety. Moreover, the experimental procedure often takes very little time compared to the time these animals have been bred to the desired age or are being held available for animal experimentation. However, for the assessment of animal welfare, the time spent waiting for an experiment or the time spent after finishing an experiment has also to be taken into account. In addition to experimental animals, many additional animals (e.g. for breeding and maintenance of genetic lines, surplus animals) are related to animal experimentation and usually face similar living conditions. Therefore, in terms of improving the overall welfare of laboratory animals, there is not only a need for refinement of experimental conditions but especially for improving living conditions outside the experiment. The improvement of animal welfare thus depends to a large extent on the housing and maintenance conditions of all animals related to experimentation. Given the current state of animal welfare research there is indeed a great potential for improving the overall welfare of laboratory animals
O mouse, where art thou? The Mouse Position Surveillance System (MoPSS)—an RFID-based tracking system
Existing methods for analysis of home cage-based preference tests are either time-consuming, not suitable for group management, expensive, and/or based on proprietary equipment that is not freely available. To correct this, we developed an automated system for group-housed mice based on radio frequency identification: the Mouse Position Surveillance System (MoPSS). The system uses an Arduino microcontroller with compatible components; it is affordable and easy to rebuild for every laboratory because it uses free and open-source software and open-source hardware with the RFID readers as the only proprietary component. The MoPSS was validated using female C57BL/6J mice and manual video comparison. It proved to be accurate even for fast-moving mice (up to 100% accuracy after logical reconstruction), and is already implemented in several studies in our laboratory. Here, we provide the complete construction description as well as the validation data and the results of an example experiment. This tracking system will allow group-based preference testing with individually identified mice to be carried out in a convenient manner. This facilitation of preference tests creates the foundation for better housing conditions from the animals’ perspective
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