Singapore's Opposition Community - Grassroots Activists in the Concrete Jungle

Based on data gained from qualitative research techniques, this paper presents and discusses the opinions of leading Singaporean oppositional grassroots activists about the state of play in Singaporean politics and civil society and likely developments over the next ten years. Our interviewees show that the Singaporean grassroots opposition activist community, while small, is passionate and committed to taking its country away from the right-wing authoritarian pathway. Those activists more interested in civil society and NGOs than contesting elections are eager to expand and deepen the civil society in Singapore. We also find that certain school-age opposition activists have already decided that the official establishment ideology, as taught in school textbooks, is not the reality of Singapore¿s history as they understand it. Activists will continue to focus on the income-inequality problem and human rights issues surrounding Article 377A of the Penal Code (which continues to make homosexual sexual acts between males illegal), the Internal Security Act (which allows detention without trial), and use of defamation suits by ruling-party politicians to bankrupt opposition party politicians and activists

The signal transducer IL6ST (gp130) as a predictive and 2 prognostic biomarker in breast cancer

Novel biomarkers are needed to continue to improve breast cancer clinical management and outcome. IL6-like cytokines, whose pleiotropic functions include roles in many hallmarks of malignancy, rely on the signal transducer IL6ST (gp130) for all their signalling. To date, 10 separate independent studies based on the analysis of clinical breast cancer samples have identified IL6ST as a predictor. Consistent findings suggest that IL6ST is a positive prognostic factor and is associated with ER status. Interestingly, these studies include 4 multigene signatures (EndoPredict, EER4, IRSN-23 and 42GC) that incorporate IL6ST to predict risk of recurrence or outcome from endocrine or chemotherapy. Here we review the existing evidence on the promising predictive and prognostic value of IL6ST. We also discuss how this potential could be further translated into clinical practice beyond the EndoPredict tool, which is already available in the clinic. The most promising route to further exploit IL6ST’s promising predicting power will likely be through additional hybrid multifactor signatures that allow for more robust stratification of ER+ breast tumours into discrete groups with distinct outcomes, thus enabling greater refinement of the treatment-selection process

Fast Domain Growth through Density-Dependent Diffusion in a Driven Lattice Gas

We study electromigration in a driven diffusive lattice gas (DDLG) whose continuous Monte Carlo dynamics generate higher particle mobility in areas with lower particle density. At low vacancy concentrations and low temperatures, vacancy domains tend to be faceted: the external driving force causes large domains to move much more quickly than small ones, producing exponential domain growth. At higher vacancy concentrations and temperatures, even small domains have rough boundaries: velocity differences between domains are smaller, and modest simulation times produce an average domain length scale which roughly follows $L \sim t^{\zeta}$, where $\zeta$ varies from near .55 at 50% filling to near .75 at 70% filling. This growth is faster than the $t^{1/3}$ behavior of a standard conserved order parameter Ising model. Some runs may be approaching a scaling regime. At low fields and early times, fast growth is delayed until the characteristic domain size reaches a crossover length which follows $L_{cross} \propto E^{-\beta}$. Rough numerical estimates give $\beta= >.37$ and simple theoretical arguments give $\beta= 1/3$. Our conclusion that small driving forces can significantly enhance coarsening may be relevant to the YB$_2$Cu$_3$O$_{7- \delta}$ electromigration experiments of Moeckly {\it et al.}(Appl. Phys. Let., {\bf 64}, 1427 (1994)).Comment: 18 pages, RevTex3.

Exposure to titanium dioxide and other metallic oxide nanoparticles induces cytotoxicity on human neural cells and fibroblasts

The use of titanium dioxide (TiO2) in various industrial applications (eg, production of paper, plastics, cosmetics, and paints) has been expanding thereby increasing the occupational and other environmental exposure of these nanoparticles to humans and other species. However, the health effects of exposure to TiO2 nanoparticles have not been systematically assessed even though recent studies suggest that such exposure induces inflammatory responses in lung tissue and cells. Because the effects of such nanoparticles on human neural cells are unknown, we have determined the putative cytotoxic effects of these nanoparticles on human astrocytes-like astrocytoma U87 cells and compared their effects on normal human fibroblasts. We found that TiO2 micro- and nanoparticles induced cell death on both human cell types in a concentration-related manner. We further noted that zinc oxide (ZnO) nanoparticles were the most effective, TiO2 nanoparticles the second most effective, and magnesium oxide (MgO) nanoparticles the least effective in inducing cell death in U87 cells. The cell death mechanisms underlying the effects of TiO2 micro- and nanoparticles on U87 cells include apoptosis, necrosis, and possibly apoptosis-like and necrosis-like cell death types. Thus, our findings may have toxicological and other pathophysiological implications on exposure of humans and other mammalian species to metallic oxide nanoparticles

Detection of radio emission from stars via proper-motion searches

We present a method for identifying radio stellar sources using their proper-motion. We demonstrate this method using the FIRST, VLASS, RACS-low and RACS-mid radio surveys, and astrometric information from Gaia Data Release 3. We find eight stellar radio sources using this method, two of which have not previously been identified in the literature as radio stars. We determine that this method probes distances of ~90pc when we use FIRST and RACS-mid, and ~250pc when we use FIRST and VLASS. We investigate the time baselines required by current and future radio sky surveys to detect the eight sources we found, with the SKA (6.7 GHz) requiring <3 years between observations to find all eight sources. We also identify nine previously known and 43 candidate variable radio stellar sources that are detected in FIRST (1.4 GHz) but are not detected in RACS-mid (1.37 GHz). This shows that many stellar radio sources are variable, and that surveys with multiple epochs can detect a more complete sample of stellar radio sources.Comment: 20 pages, 6 figures, 6 tables, accepted for publication in PAS

A matched-filter approach to radio variability and transients: searching for orphan afterglows in the VAST Pilot Survey

Radio transient searches using traditional variability metrics struggle to recover sources whose evolution timescale is significantly longer than the survey cadence. Motivated by the recent observations of slowly evolving radio afterglows at gigahertz frequency, we present the results of a search for radio variables and transients using an alternative matched-filter approach. We designed our matched-filter to recover sources with radio light curves that have a high-significance fit to power-law and smoothly broken power-law functions; light curves following these functions are characteristic of synchrotron transients, including "orphan" gamma-ray burst afterglows, which were the primary targets of our search. Applying this matched-filter approach to data from Variables and Slow Transients Pilot Survey conducted using the Australian SKA Pathfinder, we produced five candidates in our search. Subsequent Australia Telescope Compact Array observations and analysis revealed that: one is likely a synchrotron transient; one is likely a flaring active galactic nucleus, exhibiting a flat-to-steep spectral transition over $4\,$months; one is associated with a starburst galaxy, with the radio emission originating from either star formation or an underlying slowly-evolving transient; and the remaining two are likely extrinsic variables caused by interstellar scintillation. The synchrotron transient, VAST J175036.1$-$181454, has a multi-frequency light curve, peak spectral luminosity and volumetric rate that is consistent with both an off-axis afterglow and an off-axis tidal disruption event; interpreted as an off-axis afterglow would imply an average inverse beaming factor $\langle f^{-1}_{\text{b}} \rangle = 860^{+1980}_{-710}$, or equivalently, an average jet opening angle of $\langle \theta_{\textrm{j}} \rangle = 3^{+4}_{-1}\,$deg.Comment: 20 pages, 6 figures; accepted for publication in MNRA

Treatment of human astrocytoma U87 cells with silicon dioxide nanoparticles lowers their survival and alters their expression of mitochondrial and cell signaling proteins

Recent evidence suggests silicon dioxide micro- and nanoparticles induce cytotoxic effects on lung cells. Thus, there is an increasing concern regarding their potential health hazard. Nevertheless, the putative toxicity of nanoparticles in mammalian cells has not yet been systematically investigated. We previously noted that several metallic oxide nanoparticles exert differential cytotoxic effects on human neural and nonneural cells. Therefore, we hypothesized that silicon dioxide nanoparticles induce cytotoxicity in U87 cells by lowering their survival by decreasing cell survival signaling and disturbing mitochondrial function. To investigate this hypothesis, we determined the activities of the key mitochondrial enzymes, citrate synthase and malate dehydrogenase, in astrocytoma U87 cells treated with silicon dioxide nanoparticles. In addition, we studied the expression of the mitochondrial DNA-encoded proteins, cytochrome C oxidase II and nicotinamide adenine dinucleotide (NADPH) dehydrogenase subunit 6, and cell signaling pathway protein extracellular signal-regulated kinase (ERK) and phosphorylated ERK in treated U87 cells. The activated form of ERK controls cell growth, differentiation, and proliferation. In parallel, we determined survival of U87 cells after treating them with various concentrations of silicon dioxide nanoparticles. Our results indicated that treatment with silicon dioxide nanoparticles induced decreases in U87 cell survival in a dose-related manner. The activities of citrate synthase and malate dehydrogenase in treated U87 cells were increased, possibly due to an energetic compensation in surviving cells. However, the expression of mitochondrial DNA-encoded cytochrome C oxidase subunit II and NADH dehydrogenase subunit 6 and the cell signaling protein ERK and phosphorylated ERK were altered in the treated U87 cells, suggesting that silicon dioxide nanoparticles induced disruption of mitochondrial DNA-encoded protein expression, leading to decreased mitochondrial energy production and decreased cell survival/proliferation signaling. Thus, our results strongly suggest that the cytotoxicity of silicon dioxide nanoparticles in human neural cells implicates altered mitochondrial function and cell survival/proliferation signaling

A functional genomics screen reveals a strong synergistic effect between docetaxel and the mitotic gene DLGAP5 that is mediated by the androgen receptor

Based on a molecular classification of prostate cancer using gene expression pathway signatures, we derived a set of 48 genes in critical pathways that significantly predicts clinical outcome in all tested patient cohorts. We tested these genes in a functional genomics screen in a panel of three prostate cancer cell lines (LNCaP, PC3, DU145), using RNA interference. The screen revealed several genes whose knockdown caused strong growth inhibition in all cell lines. Additionally, we tested the gene set in the presence of docetaxel to see whether any gene exhibited additive or synergistic effects with the drug. We observed a strong synergistic effect between DLGAP5 knockdown and docetaxel in the androgen-sensitive line LNCaP, but not in the two other androgen-independent lines. We then tested whether this effect was connected to androgen pathways and found that knockdown of the androgen receptor by si-RNA attenuated the synergy significantly. Similarly, androgen desensitized LNCaP-AI cells had a higher IC50 to docetaxel and did not exhibit the synergistic interaction. Short-term exposure to enzalutamide did not significantly alter the behaviour of parental LNCaP cells. An immunofluorescence analysis in LNCaP cells suggests that under the double insult of DLGAP5 knockdown and docetaxel, cells predominantly arrest in metaphase. In contrast, the knockdown of the androgen receptor by siRNA appears to assist cells to progress through metaphase in to anaphase, even in the presence of docetaxel. Our data suggest that DLGAP5 has a unique function in stabilizing spindle formation and surviving microtubule assault from docetaxel, in an androgen-regulated cell cycle system

A dispositional approach to psychological climate: relationships between interpersonal harmony motives and psychological climate for communication safety

This study examined the dispositional antecedents of a climate at the individual level, psychological climate for communication safety. The impact of two interpersonal harmony motives, harmony enhancement and disintegration avoidance, on psychological climate for communication safety, innovative performance and the moderated mediated processes associated with job autonomy were examined in a survey study in China. Results showed that harmony enhancement was positively related to innovative performance through psychological climate for communication safety. Moreover, job autonomy moderated the relationship between harmony motives and psychological climate for communication safety. Harmony enhancement was more strongly associated with psychological climate for communication safety when job autonomy was low. The relationship between disintegration avoidance and psychological climate for communication safety was positive when job autonomy was high, but negative when job autonomy was low. Conditional indirect effects consistent with these interaction effects were also found