Seit die Liebste mir entfernt (c1851)

Josephine Lang Seit die Liebste mir entfernt (c1851) text by Heinrich Heine, Poem 35 of Lyrisches Intermezzo (Buch der Lieder

Characterization Of The Specific Pyruvate Transport System Of Escherichia Coli K12

A mutant of Escherichia coli K12 lacking pyruvate dehydrogenase and phosphoenolpyruvate synthase was used to study transport of pyruvate by whole cells. Uptake of pyruvate is maximal with mid-log phase cells and the Michaelis constant for transport is 20{dollar}\mu{dollar}M. Pretreatment of the cells with respiratory chain poisons or uncouplers, with the exception of arsenate, inhibits transport of pyruvate by up to 95%. Lactate and alanine, natural analogs of pyruvate, competitively inhibit transport only at very high concentrations. The synthetic analogues 3-bromopyruvate and pyruvic acid methyl ester are good competitive inhibitors.;In order to further minimize metabolism of pyruvate, membrane vesicles of a wild type E. coli K12 were prepared. Transport is dependent on an artificial electron donor system, phenazine methosulfate and sodium ascorbate, added to the vesicles. Pyruvate is concentrated 7-15 times in these energized vesicles and the Michaelis constant is 15{dollar}\mu{dollar}M. Uptake of pyruvate can also be energized by a phenazine methosulfate and NADH system, but not by the metabolic intermediates lactate, glucose or ATP. Energy poisons, with the exception of arsenate, inhibit the transport of pyruvate. Synthetic analogues such as 3-bromopyruvate are good competitive inhibitors of transport. Lactate initially appeared to be a good competitive inhibitor of pyruvate transport in vesicles, but under conditions in which the oxidation of lactate to pyruvate is minimized, this apparent inhibition disappears.;Transport of pyruvate in whole cells was also found to be sensitive to osmotic shock, indicating that a periplasmic binding protein was involved in the transport system. Column chromatography techniques were employed in an attempt to isolate this binding protein from osmotic shock fluid. Partial purification of pyruvate binding activity was achieved through the use of an affinity column, but purification to homogeneity was not accomplished with subsequent column steps. The binding protein has been tentatively identified as a 36,000 molecular weight moiety by SDS-polyacrylamide gel electrophoresis.;A brief summary of these results indicates that pyruvate is transported in Escherichia coli by a unique and specific active transport system. This transport system utilizes the respiratory chain to provide the driving force for uptake and appears to have a periplasmic component capable of binding pyruvate

Digital Micro-Credential Efficacy and Impact on Learner Confidence

This white paper details the findings of a research study conducted in 2022 over a six month period, in collaboration with a group of international cross-sector partners, as part of a Global Victoria EdTech Innovation Alliance initiative. Edalex’s Innovation Sprint aimed to increase learners’ confidence in the expression of their workplace skills by issuing a Personal Evidence Record of the skills they had developed in their studies. This evidence could then be shared with employers, sending a signal to hire by demonstrating workplace readiness or signal of recognition in the workplace of upskilling. This research validated the proof of concept of the expected efficacy of our Credentialate platform. But what we didn’t expect was the extent of the effectiveness of our solution on increasing learner confidence. The research results show that learners readily embraced the more detailed information included in the credential - such as a detailed description of the credential components, how learners were assessed and the links out to Rich Skill Descriptors (RSDs) that provided job market context. Credentialate’s Personal Evidence Record gave learners next-level understanding of what they had learnt and how they could apply it in their careers. They felt informed and empowered, which for the University of Dayton cohort had a positive impact on 76% of learner confidence levels. Employers, too, were very open to the deeper story the evidence records told. They told us that it gave them insight into the learner’s level of human capability. This is particularly valuable in graduate hiring, as it provides independent validation that they’re ready for the workplace, setting them apart from other candidates. The research project provided the opportunity to share knowledge and practice across providers and EdTech organizations and generate new ways of working in the emerging areas of micro-credential and skills ecosystems. The insights from the research should inform future policy and practice around skill transparency and personal evidence of learning, and their benefits to participants in the digital credentialing and skills ecosystems as well as the learner/earner ecosystem

Finding common ground : enhancing interaction between domestic and international students - guide for academics

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International sport federations’ commercialisation : a qualitative comparative analysis

Research question: This study examines the conditions and configurations that particularly influence International Federations’ (IFs) commercialisation. Research method: Crisp-set qualitative comparative analysis (csQCA) is used to determine the conditions that are related to an IFs’ commercialisation. Sixteen interviews were conducted in six Olympic IFs and one international sport umbrella organisation. Results and findings: The findings reveal a variety of high and low commercialisation configurations. Specialisation is a key condition in both high and low commercialisation, and social media engagement is central in high commercialisation. Strategic planning and low accountability have low degrees of overlap with high commercialisation outcomes. With 13 out of 22 IFs achieving high levels of commercialisation, the findings demonstrate that IFs are increasingly developing business-like behaviours. Implications: The findings highlight the importance of specialisation and social media engagement to achieve high commercialisation. However, when IFs assume a monetisation agenda, there are associated risks such as stakeholder legitimacy, mission drift, goal vagueness and adherence to good governance principles

Challenges and opportunities arising from self-regulated professionalisation processes: an analysis of a Swiss national sport federation

In recent decades, some governments (e.g. Canada, the UK, Australia) have imposed far-reaching professionalisation processes on national sport federations (NSFs), while others (e.g. Switzerland) have made only minor impositions and relied more on NSFs to self-regulate. As governments must decide on the extent to which sport policy imposes professionalisation processes on NSFs, understanding the challenges and opportunities arising from both policy-imposed and self-regulated professionalisation processes is relevant. However, extant literature has focused mostly on professionalisation processes imposed by sport policy. Therefore, this study aims to analyse the context, action, content and outcome of self-regulated professionalisation processes to identify the challenges and opportunities arising from these processes. A framework of professionalisation and a corresponding processual approach build the conceptual background of this study. A single-case study is applied to enable a holistic and long-term analysis of the proceedings of a Swiss NSF’s professionalisation processes. The results reveal the mechanisms of self-regulated professionalisation processes (i.e. how contexts and actions shape outcome), thus leading to a conceptualisation of these mechanisms and conclusions about challenges and opportunities arising from selfregulated professionalisation processes, which are useful for sport managers and policymakers

Drivers of and Barriers to Professionalization in International Sport Federations

In a changing and complex environment, international sport federations (IFs) have to face new challenges. These challenges can trigger or hinder IFs’ professionalization processes. While researchers have examined organizational change and professionalization of national sport federations (NFs) and clubs, studies on IFs are rare. Considering professionalization as an important element of IFs’ change processes in recent years, the study attempts to fill this gap. The conceptual framework is based on the concepts and dynamics of organizational change, the influence of isomorphic pressures and the operationalization of a multi-level framework. Data from six case studies was analyzed by means of qualitative content analysis. Findings reveal multiple causes of IFs’ professionalization. Three particular findings are discussed: professionalization as a dynamic process with phases of acceleration that vary depending on IFs’ size; IFs’ becoming increasingly business-like through isomorphic changes; and five causes of particular relevance to IFs’ current professionalization process

Causes for professionalization in national sport federations in Switzerland: a multiple-case study

Aim and design: This study explores causes for professionalization in Swiss national sport federations (NSFs). We conducted a multiple-case study employing a qualitative approach with interviews and documents from seven NSFs. A three-level framework guided the analysis in distinguishing NSF endogenous causes, and causes in their external and internal environment.Results: Causes for professionalization were widely similar in the NSFs. Conflicts on the board, unclear decision-making competences and initiatives of key persons have prominently triggered professionalization, particularly for differentiation of strategic boards and executive headquarters, specialization and paid staff. The Swiss government, Swiss Olympic Association and sponsors (external environment) have brought about considerable adaptations in NSFs’ strategies, accountability issues and commercialization, whereas expectations of NSFs’ member organizations (internal environment) have had little impact on their professionalization in general. Rather, the NSFs view them as necessities they themselves do not perceive. Our analysis revealed additional NSF-specific factors (e.g., popularity, financial resources, attitude of individuals towards professionalization) that have an impact on pace and continuity of the process once it has been initiated.Conclusion: Our analysis is a first step towards understanding the professionalization process in Swiss NSFs. Referring to the similar causes for professionalization, uncertainty and competing for resources may have led some NSFs to mimic those NSFs they deem successful, leading to similar conditions that call for professionalization (e.g., with respect to workload and internal expectations). Respective networks between motivated people in the NSFs and stakeholders could support a deliberate professionalization. NSF endogenous and NSF external causes for professionalization seem to be reciprocal. This should be considered more specifically in the analysis of professionalization in NSFs. Single-case studies would be useful to understand the mechanisms and eventual phases more clearly, to identify eventual barriers and avoid unintended consequences to, finally, support NSFs’ professionalization in an efficient manner

Types of professionalization: understanding contemporary organizational designs of Swiss national sport federations

Purpose – The purpose of this paper is to identify types of professionalization in Swiss national sport federations (NSFs) and analyze organizational characteristics associated with specific types of professionalization. Such types reveal common patterns among the increasingly complex organizational designs of NSFs and thus contribute to the understanding of professionalization in NSFs. Design/methodology/approach – An online survey of all Swiss NSFs was conducted to identify types of professionalization in these organizations using hierarchical cluster analysis, based on a multi-dimensional framework of professionalization. Findings – The analysis revealed four types of professionalization: formalized NSFs managed by paid staff, NSFs managed by volunteers and a few paid staff off the field, NSFs with differing formalization and paid staff on the field, and moderately formalized NSFs managed by volunteers. The types differ in terms of the NSFs’ organizational characteristics, in particular, size, financial resources, Olympic status, and performance. Originality/value – Applying factor and cluster analysis is a new approach to analyzing professionalization in NSFs that makes uncovering distinctive organizational patterns among a large number of NSFs possible. These results lay the foundation for understanding the professionalization of NSFs, counseling NSFs on their organizational development, and conducting future research on the design types of sport organizations