Application of effect-based methods (EBMs) in a river basin: a preliminary study in Central Italy

Introduction. Effect-based methods (EBMs), i.e. in vitro and in vivo bioassays, represent innovative tools for the effect detection of environmental chemical pollutants on living organisms. The aim of this study was to evaluate the water quality of a river ecosystem implementing two in vivo bioassays on target freshwater animal species: the crustacean Daphnia magna and the small fish Danio rerio, also known as zebrafish. Materials and methods. The methods applied in this study, i.e. the Daphnia sp. Acute Immobilisation assay and the Fish Embryo Acute Toxicity (FET) test, are commonly used in water quality research and their application in short-term ecotoxicity detection is suggested by recent European projects. Two sampling sites were chosen in the urban part of the Tiber River in Rome, while a third one was chosen as a reference site in the Farfa River, a tributary upstream of the city. The sites in the Tiber River are potentially affected by different pollution sources, including urban and industrial wastewater discharges, the pesticide release, livestock waste products, and waste dumps. Results and discussion. The results of the study showed wide differences between the two applied bioassays. The FET test was generally more sensitive in detecting even low effects in all the water samples, but the strongest statistically results were observed with the D. magna Acute Immobilisation test. The results of this research confirm the effectiveness of EBMs in investigating and monitoring water chemical pollution, and stress the need for performing further studies, e.g. chemical analyses and other bioassays, to improve the knowledge of the health status of the Tiber River basin. Conclusions. Further results will aim to support the local authorities in adopting measures to reduce and to eliminate the sources of chemical pollution in the study area

Fish-Kills in the Urban Stretch of the Tiber River After a Flash-Storm: Investigative Monitoring with Effect-Based Methods, Targeted Chemical Analyses, and Fish Assemblage Examinations

In 2020 and 2021, fish-kills events occurred in the Tiber river in the city of Rome. These events, which caused the death of thousands of fish of different species (e.g., Barbus spp., Cyprinus carpio, Squalius spp.), were preceded the days before by severe flash-storms. Heavy rains in urban areas in recent years are linked to climate change and fish-kills events. With the aim to investigate the causes of these events, effect-based methods (EBMs) and targeted chemical analyses have been performed on a specific site of river Tiber in the center of the city. Additionally, examination and classification of the floating dead fishes have been performed. The chemical analysis performed on several groups of contaminants showed the presence of some pharmaceuticals, insecticides, and PFAS at up to ng/L. Results with the Fish Embryo Toxicity (FET) test confirmed lethal and sub-lethal effects, while acute effects were not detected with the Daphnia magna acute test. The fish-kills events probably can be generated by several factors as a consequence of an increasing human anthropization of the area with the contribution of different stressors together with chemical releases and emissions. This study shows that in multiple stressor scenarios characterized by heavy rainfall, droughts, and strong anthropogenic pressures, the application of EBMs, chemical analysis, and fish assemblage examinations can represent a useful support in the investigation of the causes of extensive fish-kills events

Smoking behaviour predicts tobacco control attitudes in a high smoking prevalence hospital: A cross-sectional study in a Portuguese teaching hospital prior to the national smoking ban

<p>Abstract</p> <p>Background</p> <p>Several studies have investigated attitudes to and compliance with smoking bans, but few have been conducted in healthcare settings and none in such a setting in Portugal. Portugal is of particular interest because the current ban is not in line with World Health Organization recommendations for a "100% smoke-free" policy. In November 2007, a Portuguese teaching-hospital surveyed smoking behaviour and tobacco control (TC) attitudes before the national ban came into force in January 2008.</p> <p>Methods</p> <p>Questionnaire-based cross-sectional study, including all eligible staff. Sample: 52.9% of the 1, 112 staff; mean age 38.3 ± 9.9 years; 65.9% females. Smoking behaviour and TC attitudes and beliefs were the main outcomes. Bivariable analyses were conducted using chi-squared and MacNemar tests to compare categorical variables and Mann-Whitney tests to compare medians. Multilogistic regression (MLR) was performed to identify factors associated with smoking status and TC attitudes.</p> <p>Results</p> <p>Smoking prevalence was 40.5% (95% CI: 33.6-47.4) in males, 23.5% (95% CI: 19.2-27.8) in females (p < 0.001); 43.2% in auxiliaries, 26.1% in nurses, 18.9% among physicians, and 34.7% among other non-health professionals (p = 0.024). The findings showed a very high level of agreement with smoking bans, even among smokers, despite the fact that 70.3% of the smokers smoked on the premises and 76% of staff reported being frequently exposed to second-hand smoke (SHS). In addition 42.8% reported that SHS was unpleasant and 28.3% admitted complaining. MLR showed that smoking behaviour was the most important predictor of TC attitudes.</p> <p>Conclusions</p> <p>Smoking prevalence was high, especially among the lower socio-economic groups. The findings showed a very high level of support for smoking bans, despite the pro-smoking environment. Most staff reported passive behaviour, despite high SHS exposure. This and the high smoking prevalence may contribute to low compliance with the ban and low participation on smoking cessation activities. Smoking behaviour had greater influence in TC attitudes than health professionals' education. Our study is the first in Portugal to identify potential predictors of non-compliance with the partial smoking ban, further emphasising the need for a 100% smoke-free policy, effective enforcement and public health education to ensure compliance and promote social norm change.</p

Aquatic Ecosystems and Climate Changes: Data Gaps in Relation to Flooding Events

at european level the strategy on adaptatio to climate chenges promoted adaptation plans at all levels and has recommended the need to bridge the gaps through applicaitons of actions at local level. In the paper has been remarked that there are some gaps in the European legislation that should be solved through the funding of more research projects and pilot studies; for example the use of microbiological monitoring, not foreseen by the water framework directive, can help to identify and prevent effect adverse on human health. In this study we propose an operative methodology to be applied at local level with the aim to support the institutional requirements for risk management before and after flooding events, based on a multi-criteria approach. This integrated monitoring plan coul represent a shared methodology with the aim to give a contribution for the management of the risks of extreme weather events such as flooding for human health and ecosystem protection

Hydrocarbon contaminated soil treated by bioremediation technology: microbiological and toxicological preliminary findings

Soil contamination is a significant factor in the general degradation of the environment. Remedial intervention on contaminated soils may be carried out bymeans of specific and different technologies, including chemical, physical and biological methodologies. Of these, the latter are the least invasive and bioremediation intervention can be carried out both in situ and ex situ. The object of this study was a clay soil highly contaminated by heavy hydrocarbons and polycyclic aromatic hydrocarbons (PAHs). The soil was subjected to remediation treatment using a bioremediation technique, applied by inoculating a nutritive and enzymatic-bacterial mixture. A qualitative-quantitative microbial characterization of the soil matrix was undertaken, both before, and thirty days after, the treatment. The percentage abatement in hydrocarbon concentration and the overallmicrobial concentration present in the soil were measured, and the eco-toxicological impact was estimated. The eco-toxicological aspect was assessed by applying the ostracod test and the phytotoxicity test. The microbial component was estimated by the Direct Count method on plates: this method highlighted the vital countable cells of telluric and degrader mesophilic bacteria, of the Pseudomonadaceae, of the actinomycetes and total mycetes (moulds and yeasts). This study was focused on evaluating biological remedial technology combining chemical analysis with microbiological assessment and bioassays, because chemical analysis alone cannot provide a full picture of the bioremediation process and its effectiveness. The data obtained indicated that the bioremediation technique applied gave optimal results in terms of percentage abatement of the hydrocarbon concentration, microbial enrichment of the soil matrix and reduction of toxicity found in the soil before the clean-up treatment

Mercury (Hg) and methylmercury (MeHg) in sediment and biota: A case study in a lagoon in Central Italy

A quantification of total mercury (Hgtot) and methylmercury (MeHg) concentrations in sediment and mussels was carried out in the east basin of the Orbetello lagoon in order to assess their bioaccumulation potential. The sediment was sampled in four macroareas, mussels were transplanted in the same sites and collected after seven weeks. The results show that Hgtot concentrations in sediments exceeded (0.21–16.9 mg/kg dry weight (dw)) the environmental quality standard of the Italian legislation (0.3 mg/kg dw). The Hgtot concentration in mussels (0.050–0.324 mg/kg wet weight (ww)) does not exceed the limit values (0.5 mg/kg ww) of the European food legislation. The biota–sediment accumulation factors (BSAFs) derived for MeHg (80–306.7) and a biomagnification factor (BMF) greater than 1 for Hgtot demonstrate that in the lagoon, these compounds can be transferred in the upper levels of the trophic chain and pose a risk to human health

A new supported TiO2 film deposited on stainless steel for the photocatalytic degradation of contaminants of emerging concern

A new supported catalyst composed of a nanostructured TiO2 film deposited on a stainless steel mesh (nanoTiO2-SS) using the Metal Organic Chemical Vapour Deposition (MOCVD) technique was evaluated for the photocatalytic degradation of a mixture of contaminants of emerging concern. Results showed that under the oxidative conditions tested, the nanoTiO2-SS catalyst demonstrated an efficiency in degrading the target contaminants higher than that observed under direct photolysis and photocatalysis using the conventional TiO2 Degussa P25 catalyst at the same amount of TiO2 participating to the photocatalysis. Specifically, the rate of removal of warfarin and trimethoprim obtained with the new catalyst was found twice the one observed by using TiO2 Degussa P25 and approximately 1.6 times faster for metoprolol, carbamazepine and gemfibrozil. An evaluation of the electrical energy per order magnitude of removal (EE/O) confirmed the enhanced performance of the new catalyst (24.3–31.8 kWh m−3 rather than 32.8–39.3 kWh m−3 for conventional TiO2) and that the performance is compound-dependent. Toxicity testing revealed that some assays are suitable for the investigation of bioactivity of treated waters containing contaminants of emerging concern at μg L−1 level. Specifically, the AMES Fluctuation Test, Fish Embryo Acute Toxicity Test and Green alga Selenastrum capricornutum test provided valuable results for an environmental impact assessment. On the other hand, the Daphnia magna and Vibrio fischeri acute toxicity tests were not sensitive enough to detect bioactivity in the samples analysed without prior pre-concentration