Testicular activity and sperm glycoproteins in giant red shrimp Aristaeomorpha foliacea

The reproduction of male giani red shrimp Aristaeomorpha foliacea, collected from thè late winterto thè summer in thè north-western lonian Sea (Mediterranean Sea), was investigated using histological and histochemistry methods. Seasonal changes in thè spermiogenesis and thè glycoprotein pattern were found and sperm glycoproteins matured as gametes moved from thè testis to thè terminal ampliila. In serial sections stained with hematoxylin and eosin thè testicular activity appeared to be discontinuous. In late winter thè testes had no meiotic activity and thè seminiferous epithelium consisted of interkinetic spermatogonia and spermatozoa. In spring, spermiogenetic activity was high and thè seminiferous epithelium mainly consisted of spermatocytes and spermatozoa while in summer, thè testes were again inactive since both spermatocytes and spermatozoa were lacking. The use of twelve different lectins indicated that thè intratesticular spermatozoa from late winter to summer contain surface binding sites for SNA, MAA, Con A and KOH-sialidase (si)-WGA. In March and July they also exhibited nuclear and cytoplasmic reactivity for SNA and Con A. In thè hemispermatophore thè spermatozoa displayed a more complex lectin-binding pattern because they also reacted with PNA, DBA, HPA, OSA II. The staining with DBA, KOH-si- DBA, and OSA II showed differences between thè spermatozoa from late winter-spring hemispermatophores and summer hemispermatophores: thè former showed a nuclear affinity whereas thè latter displayed surface and/or cytoplasm staining. No reaction was observed with SBA, GSAI-B4, UEA I, and LTA

Intra-cycle depolarization of ultraintense laser pulses focused by off-axis parabolic mirrors

A study of the structure of the electric and magnetic fields of ultraintense laser pulses focused by an off-axis parabolic mirror is reported. At first, a theoretical model is laid out, whose final equations integration allows the space and time structure of the fields to be retrieved. The model is then employed to investigate the field patterns at different times within the optical cycle, for off-axis parabola parameters normally employed in the context of ultraintense laser–plasma interaction experiments. The results show that nontrivial, complex electromagnetic field patterns are observed at the time at which the electric and magnetic fields are supposed to vanish. The importance of this effect is then studied for different laser polarizations, $f$ numbers and off-axis angles

Robust and stable region-of-interest tomographic reconstruction using a robust width prior

Region-of-interest computed tomography (ROI CT) aims at reconstructing a region within the field of view by using only ROI-focused projections. The solution of this inverse problem is challenging and methods of tomographic reconstruction that are designed to work with full projection data may perform poorly or fail when applied to this setting. In this work, we study the ROI CT problem in the presence of measurement noise and formulate the reconstruction problem by relaxing data fidelity and consistency requirements. Under the assumption of a robust width prior that provides a form of stability for data satisfying appropriate sparsity-inducing norms, we derive reconstruction performance guarantees and controllable error bounds. Based on this theoretical setting, we introduce a novel iterative reconstruction algorithm from ROI-focused projection data that is guaranteed to converge with controllable error while satisfying predetermined fidelity and consistency tolerances. Numerical tests on experimental data show that our algorithm for ROI CT is competitive with state-of-the-art methods especially when the ROI radius is small

Avaliacoes da infeccao de explantes de feijoeiro por Agrobacterium tumefaciens por meio de microscopia.

Phaseolus vulgaris L. e considerada recalcitrante a transformacao por Agrobacterium tumefaciens. Contudo, alteracoes no meio de co-cultivo, utilizacao de linhagens hipervirulentas de Agrobacterium e de vetores binarios contendo genes vir demostraram que o feijoeiro e susceptivel a essa bacteria. O objetivo do presente trabalho foi estudar o efeito da sonificacao nos tecidos vegetais de feijoeiro, bem como a penetracao da Agrobacterium nas camadas subepidermicas do tecido vegetal, usando a metodologia SAAT ("Sonification-Assisted Agrobacterium-mediated Transformation"). A variedade de feijoeiro utilizada foi a Olathe Pinto, a linhagem de A. tumefaciens foi LBA4404:pTOK.Os embrioes de feijao foram pre-tratados po 14 dias em meio de multibrotacao e, entao submetidos a sonificacao (de 0 ou 60 segundos) na presenca de Agrobacterium. Apos a inoculacao foram co-cultivados por 24 horas em meio liquido seguido de 48 horas em meio solido, ambos, contendo 20 m. L -1 de acetoceringona. Os explantes inoculados foram fixadas em solucao de Karnovsk para avaliacoes em microscopia optica e eletronica de varredura. As analises da microspia demostraram a presenca de rupturas na epiderme, quebras da parede celular e invasao da Agrobacterium nos tecidos subepidermicos. Os reultados demostraram que o metodo SAAT e uma tecnica viavel para a inoculcao de Agrobacterium em explantes de P. vulgaris.bitstream/CPAC-2010/23710/1/bolpd-04.pd

Incomplete ileocecal bypass for ileal pathology in horses: 21 cases (2012–2019)

Background: Incomplete ileocecal bypass can be performed in cases in which an ileal disfunction is suspected but resection of the diseased ileum is not necessary. Objectives: To describe the clinical findings, the surgical technique, and the outcome of 21 cases of colic with ileal pathologies that underwent an incomplete ileocecal bypass. Methods: Historical, clinical, and surgical features of cases diagnosed with pathologies involving the ileum or the ileocecal valve that underwent ileocecal anastomosis without ileal resection were retrieved. Clinical (heart rate, duration of symptoms, presence of reflux, age, weight at arrival) and surgical (surgical pathology, duration of surgery, type of anastomosis) data were retrieved and analysed. Data on short term survival and postoperative complications (colic, post-operative reflux, incisional infection, fever), length of hospital stay, and long term follow up were also obtained. Results: A total of 21 horses met the criteria; 13 horses had ileal impaction (one with muscular hypertrophy), 5 horses had epiploic foramen entrapment, and 3 horses had a pedunculated lipoma. An incomplete ileocecal bypass was performed with a two-layer hand-sewn side-to-side technique in 19 cases and with a stapled side-to-side technique in 2 cases. Short term survival was 95.2%. At 12-months follow up, all horses but two were alive, and 13 of the 14 sport horses returned to their previous level of activity. Long term survival was 90.47%. Conclusions Incomplete ileocecal bypass may represent a valid surgical technique in case of ileocecal valve disfunction when ileum resection is not necessary; this technique may represent an alternative to extensive manipulation without subsequent recurrence of ileal impaction