Coexpression of EphB4 and ephrinB2 in tumour advancement of ovarian cancers

EphB4 and ephrinB2 expressions in ovarian cancers were studied to analyse EphB4/ephrinB2 functions against clinical backgrounds. EphB4 and ephrinB2 were dominantly localised in ovarian cancer cells of all cases studied. Both the histoscores and mRNA levels of EphB4 and ephrinB2 significantly increased with clinical stages (I<II<III<IV, P<0.001) in ovarian cancers, although there was no significant difference in EphB4 and ephrinB2 histoscores or in mRNA levels according to histopathological types. EphB4 as well as ephrinB2 histoscores in cancer cells correlated with the corresponding mRNA levels in each case (EphB4, P<0.001; ephrinB2, P<0.001). The 24-month survival rates of the 36 patients with high EphB4 and ephrinB2 expression were poor (25 and 27%, respectively), while for the other 36 patients with low EphB4 and ephrinB2 expression, they were significantly higher (68 and 64%, respectively). Therefore, EphB4/ephrinB2 may function in tumour advancement and coexpression of the Eph/ephrin system may potentiate tumour progression leading to poor survival. Thus, EphB4/ephrinB2 can be recognised as a novel prognostic indicator in the primary tumours of ovarian cancers

Constitutive model describing the hydro-mechanical behaviors of compacted bentonite clay based on the crystal surface phenomena

A constitutive model for unsaturated expansive soil based on the crystal surface phenomena is proposed. The behavior of the proposed model is described as a double structure, with the soil skeleton and the interlaminar behaviors. The soil skeleton behavior is modeled by the Cam clay type model based on the Bishop’s effective stress, which can consider the degree of saturation-induced hardening and inelastic behavior in the over-consolidated region and is capable of describing hydraulic collapse. On the other hand, the interlaminar behavior is give as a result of the interlaminar equilibrium of clay minerals. The diffusion double layer repulsive force in the interlaminar equilibrium varies with the degree of saturation, and it mainly causes the hydraulic swelling of expansive soil. The performance of the model is validated through the simulations of the suction-controlled oedometer tests on the heavily compacted bentonite

Experimental and analytical investigations of volume change behaviour of saturated expansive soils in oedometer test

The chemical and mechanical behaviours of saturated bentonite were experimentally and analytically investigated. A series of oedometer tests on saturated bentonite was conducted. By replacing the cell fluid during the oedometer test, one-dimensional osmotic consolidation and swelling behaviour were observed. The experimental results were simulated numerically using a constitutive model by considering the influence of electrochemical phenomena of clay mineral crystals on the macrostructural behaviour. It was concluded that the typical mechanical swellability of water-saturated bentonite in oedometer test is affected by a positive dilatancy behaviour due to the lower shear strength. After the osmotic consolidation test, a lateral contraction of the specimen in the oedometer was observed; this affected the compressibility during the subsequent mechanical consolidation. It was also concluded that the concentration of Na+ in the pore fluid affects the specific mechanical properties of bentonite

Effects of loading rate on strength and deformation characteristics of gypsum mixed sand

It has been unanimously acknowledged that the strength and deformation characteristics of bounded geomaterials, viz. cemented soils and natural rocks, are predominantly governed by the rate of loading/deformation. Rational evaluation of these time-dependent characteristics due to viscosity and ageing are vital for the reliable constitutive modelling. In order to study the effects of ageing and loading/strain rate (viscosity) on the behaviour of bounded geomaterials, a number of unconfined monotonic loading tests were performed on Gypsum Mixed Sand (GMS) specimens at a wide range of axial strain rates; ranging from 1.9E-05 to 5.3E+00 %/min (27,000 folds), and at different curing periods. The results indicate shifts in the viscous behaviour of GMS at critical strain rates of 2.0E-03 and 5.0E-01 %/min. In the light of this finding, the results are categorized into three discrete zones of strain rates, and the behaviour of GMS in each of these zones is discussed. A significant dependency of peak strength and stress-strain responses on strain rate was witnessed for specimens subjected to strain rates lesser than 2.0E-03 %/min, and the effects of viscosity/strain rate was found to be insignificant at strain rate higher than 5.0E-01%/min

Isogeometric analysis of THM coupled processes in ground freezing

An isogeometric analysis (IGA) based numerical model is presented for simulation of thermo-hydro-mechanically (THM) coupled processes in ground freezing. The momentum, mass and energy conservation equations are derived based on porous media theory. The governing equations are supplemented by a saturation curve, a hydraulic conductivity model and constitutive equations. Variational and Galerkin formulation results in a highly nonlinear system of equations, which are solved using Newton-Raphson iteration. Numerical examples on isothermal consolidation in plane strain, one-dimensional freezing and heave due to a chilled pipeline are presented. Reasonably good agreements were observed between the IGA based heave simulations and experimental results.acceptedVersio

Water and soil particle movement in unsaturated bentonite with constrained and free swelling boundaries

Bentonite is considered a barrier material in the geological disposal of high-level radioactive waste. Depending on the construction method, it is necessary to know what the behavior of the bentonite barrier will be in the wetting process during the absorption of underground water under different boundary conditions. In this study, water and soil particle movement during the wetting process of a compacted bentonite under constrained and free swelling conditions was studied experimentally and numerically. For the constrained swelling boundary condition, in which the swelling deformation was fully constrained, the distributions of the gravimetric water content (w) were measured for specimens with different dry densities (ρd), and then the water diffusivity (Dw) was obtained. It was found that Dw showed a slightly decreasing trend with an increase in ρd, while the numerical simulations showed that the difference in Dw induced by ρd was minor in terms of the evolving degree of saturation in the tested range. For the free swelling boundary condition, the distributions of w and ρd were measured for specimens with an initial ρd of 1.6 Mg/m3. To obtain Dw and the soil particle diffusivity (Ds), an existing theoretical framework, to which new concrete calculation equations had been added, was presented. Then, the performance of the framework was examined by numerical simulations to illustrate the water and soil particle movement under conditions similar to those of the experiment. It was found that the framework can describe the experimental results well, but that the accuracy of the results largely depends on the accuracy of the experimental data