New Secondary Metabolites From Quercus Coccifera L.

Three new secondary metabolites kermesoside (1), cocciferoside (2) and (-)-8-chlorocatechin (3), were isolated from the the stems with barks of Quercus coccifera along with five known phenolic compounds, 3-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-propan-1one (4) and 3-hydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-propan-1-one (5), trans-resveratrol-3-O-beta-glucopyranoside (6) lyoniresinol-9-O-beta-xylopyranoside (7), lyoniresinol-9-O-beta-glucopyranoside (8). The structure elucidation of the isolated compounds was performed by spectroscopic methods (UV, 1D- and 2D- NMR and HR-MS).Wo

Isolation And Structure Elucidation Of Uncommon Secondary Metabolites From Cistus Salviifolius L.

To our knowledge this is the first report on the isolation of a flavonoid glycoside: quercetin 3-O-alpha-arabinopyranoside (5), two phenylbutanon glycosides: 4-(4'-O-[6''-O-galloyl-beta-galactopyranosyl]-3'-hydroxyphenyl)-butan-2-on (8), 4-(3'-O-beta-glucopyranosyl-4'-hydroxyphenyl)-butan-2-on (9), one phloroglucinol glycoside: 1-O-beta-glucopyranosyl-3,5-dimethoxybenzene (10) and a steroid glycoside: sitosterol-3-O-(6''-O-butanoyl)-beta-galactopyranoside (14) from the Cistus species (Cistaceae). Additional to these compounds three flavonol aglycones: kaempferol (1), quercetin (2), myricetin (3); three flavonoid glycosides; kaempferol 3-O-beta-(6''-O-trans-p-coumaroyl)-glucopyranoside (4), quercetin 3-O-beta-galactopyranoside (6), myricetin 3-O-beta-galactopyranoside (7); one phloroglucinol glycoside: 1-O-beta-glucopyranosyl-3,5-dimethoxybenzene (11); one steroid aglycone: beta-sitosterol (12); one steroid glycoside: Sitosterol-3-O-beta-glucopyranoside (13) were isolated from the aerial parts of the Cistus salviifolius L.. Their structures were identified using spectral methods (UV, IR, 1D- and 2D-NMR, and ESI-MS).Wo

Phenylpropanoids, Sesquiterpenoids And Flavonoids From Pimpinella Tragium Vill. Subsp Lithophila (Schischkin) Tutin

A new sesquiterpenoid named germacradiene-6-O-(6'-O-acetyl)-beta-D-glucoside (1) and a new flavonol glycoside named rhamnetin-3-O-(2 ''-O-beta-D-glucopyranosyl)-beta-D-galactopyranoside (2), along with three known sesquiterpenoids dictamnol (3), radicol (4), germacradiene glucoside (5); three phenylpropanoids 4-methoxy-2-(3-methyloxiranyl)-phenyl 2-methylbutanoate (6), 4-methoxy-2-(3-methyloxiranyl)-phenyl angelate (7), thellungianin E (8); and a flavonol glycoside platanoside (9) were isolated from the aerial parts of Pimpinella tragium Vill. subsp. lithophila (Schischkin) Tutin. Their structures were elucidated by detailed analyses of 1D and 2D NMR, UV, IR and HR-ESI-MS data.Wo

Phenylacylated-flavonoids from Peucedanum chryseum

Phytochemical investigation of the methanol extract of the aerial parts of Peucedanum chryseum (Boiss. & Heldr.) D.F.Chamb., Apiaceae, led to the isolation of a dihydrofuranochromone, cimifugin (1); a phloroacetophenone glucoside, myrciaphenone A (2); and a flavonoid glycoside, afzelin (3) along with two phenylacylated-flavonoid glycosides: rugosaflavonoid C (4), and isoquercitrin 6"-O-p-hydroxybenzoate (5). The structures of compounds 1-5 were elucidated by extensive 1D- and 2D-NMR spectroscopic analysis in combination with MS experiments and comparison with the relevant literature. All compounds are reported for the first time from this species and compounds 2, 4, and 5 from the genus Peucedanum and from Apiaceae. (C) 2018 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda

The Effect Of Quercus Coccifera L. Extract On The Necrosis Of Random-Pattern Skin Flaps In Rats

Aim: Ischemia-reperfusion injury is an important cause of flap loss. We investigated effects of Quercus coccifera L. extract known to have anti-oxidant and anti-inflammatory activity on skin flap viability. Methods: Eighteen Wistar rats were used. Random-pattern skin flaps, 9x3 cm in size, were applied on rats back and then the rats were divided into three groups. Following surgery 2 cc of nitrofurazone cream and 2 cc of Q. coccifera L. water extract were applied daily in groups 2 and 3, respectively. Group 1 received no treatment after surgery. The percentage of necrosis on the flaps was calculated and histopathological examination of the tissue samples was made on day 7. Results: The mean percentage of necrosis was 44.7%, 42.5% and 21.8% group 1, 2 and 3, respectively. The percentage of necrosis was statistically significantly lower in group 3 than in groups 1 and 2 (p<0.001 and p<0.00, respectively). Neutrophil score was found to be significantly lower in group 3 than in groups 1 and 2 (p=0.002 and p=0.002, respectively). Conclusions: Necrosis of skin flaps was decreased through application of Q. coccifera extractsWoSScopu

Adulteration Determining Of Pharmaceutical Forms Of Ginkgo Biloba Extracts From Different International Manufacturers

In this study, Ginkgo biloba products used for the same purpose, but licensed to varying authorities were analyzed in point of similarity to each other. A group of these products were licensed from health authorities as herbal medicinal product (HMP), while the other groups of products were licensed as the food supplement (FS). The evaluation of their phytoequivalence was carried out comparing the chromatographic fingerprint profiles. Furthermore, ginkgolides (ginkgolides GA, GB, GC, and GJ) and flavonoid aglycones (quercetin, kaempferol, and isorhamnetin) were quantitatively analyzed by using liquid chromatography-mass spectrometry (LC-MS) and HPLC-diode Array detector (HPLC-DAD) assays. All six herbal medicinal products and two food supplements were found to be phytoequivalent to each other, but five of the seven food supplements did not possess similar content as herbal medicinal products, and the quantity of ginkgolides and flavonoid aglycones per tablet/capsule was found to be lower than declared on the labels. In addition, food supplements were found to be adultered with rutin to reach expected total flavonoid glycosides amount.Wo

Anti-inflammatory effects of different extracts from three Salvia species

Salvia L. species have been used for the treatment of various inflammatory ailments in traditional medicine. In order to evaluate this ethnobotanical information, water, methanol, n-butanol, acetone, and chloroform extracts from 3 Salvia species (S. fruticosa, S. verticillata, and S. trichoclada) were screened for their anti-inflammatory activity using in vivo experimental models in rats. For this purpose a carrageenan-induced inflammatory paw edema model was used. All extracts demonstrated anti-inflammatory activities; however, n-butanol extract of Salvia fruticosa (syn. S. triloba), which is known as Turkish sage, was found to be the most active. It can be expected that the active flavonoids, phenolic acids, and terpenoids may be responsible for the anti-inflammatory activity of these plants

Hplc Fingerprinting Of Sennosides In Laxative Drugs With Isolation Of Standard Substances From Some Senna Leaves

Senna leaves are one of the oldest medicinal herbs and they are used as laxative. Herbal teas which contain senna leaves are most commonly used to promote weight loss. The quality control of slimming teas which contain Senna leaves and also pharmaceutical preparations including Senna extract enriched by sennoside B was achieved by HPLC fingerprinting method. While the presence of sennoside A and B in laxative drugs was proved, it was seen to be devoid of sennosides in slimming teas. Kaempferol 3-O-beta-D-gentiobioside ( 1), aloe-emodine 8-O-beta-D-glucopyranoside ( 2), rhein 8-O-beta-D-glucopyranoside ( 3), torachrysone 8-O-beta-D-glucopyranoside ( 4), isorhamnetine 3-O-beta-D-gentiobioside ( 5) were also isolated from Senna leaves.Wo

Chemical Constituents of two Sages with Free Radical Scavenging Activity

From the aerial parts of Salvia trichoclada Bentham and S. verticillata L. one new and two known phenolic acids, 3-(3',4'-dihydroxyphenyl)-2-hydroxymethyl propionic acid (1), 3-(3',4'-dihydroxyphenyl) lactic acid (2), and rosmarinic acid (3); two flavonoids, apigenin 4'-methyl ether 7-O-glucuronide (4), and luteolin 7-O-beta-glucuronide (5); two lupan type triterpene aglycones, lupeol (6), and 30-hydroxylup-20 (29)-en-3-on (7); an oleanane-type triterpene acid, oleanolic acid (8); and an ursan-type triterpene acid, ursolic acid (9) were isolated. The structures of the compounds were elucidated by spectroscopic analysis. Different extracts of the plants were examined for their free radical scavenging activities by DPPH (2,2-Diphenyl-1-picrylhydrazyl) assay. Some of the polar extracts showed high free radical scavenging activity