Expression, purification, crystallization and preliminary X-ray diffraction analysis of conjugated bile salt hydrolase from Bifidobacterium longum

Conjugated bile salt hydrolase (BSH) catalyses the hydrolysis of the amide bond that conjugates bile acids to glycine and to taurine. The BSH enzyme from Bifidobacterium longum was overexpressed in Escherichia coli BL21(DE3), purified and crystallized. Crystallization conditions were screened using the hanging-drop vapour-diffusion method. Crystal growth, with two distinct morphologies, was optimal in experiments carried out at 303 K. The crystals belong to the hexagonal system, space group P622 with unit-cell parameters a = b = 124.86, c = 219.03 Angstrom, and the trigonal space group P321, with unit-cell parameters a = b = 125.24, c = 117.03 Angstrom. The crystals diffracted X-rays to 2.5 Angstrom spacing. Structure determination using the multiple isomorphous replacement method is in progress

Expression, purification, crystallization and preliminary X-ray diffraction analysis of conjugated bile salt hydrolase from Bifidobacterium longum

Conjugated bile salt hydrolase (BSH) catalyses the hydrolysis of the amide bond that conjugates bile acids to glycine and to taurine. The BSH enzyme from Bifidobacterium longum was overexpressed in Escherichia coli BL21(DE3), purified and crystallized. Crystallization conditions were screened using the hanging-drop vapour-diffusion method. Crystal growth, with two distinct morphologies, was optimal in experiments carried out at 303 K. The crystals belong to the hexagonal system, space group P622 with unit-cell parameters a = b = 124.86, c = 219.03 Angstrom, and the trigonal space group P321, with unit-cell parameters a = b = 125.24, c = 117.03 Angstrom. The crystals diffracted X-rays to 2.5 Angstrom spacing. Structure determination using the multiple isomorphous replacement method is in progress

Laminate comprising fibers embedded in cured amine terminated bis-imide

Amine terminated bisaspartimides are prepared by a Michael type reaction of an aromatic bismaleimide and an aromatic diamine in an aprotic solvent. These bisaspartimides are thermally polymerized to yield tough, resinous polymers crosslinked through -NH- groups. Such polymers are useful in applications requiring materials with resistance to change at elevated temperatures

Asymptotic Performance of Linear Receivers in MIMO Fading Channels

Linear receivers are an attractive low-complexity alternative to optimal processing for multi-antenna MIMO communications. In this paper we characterize the information-theoretic performance of MIMO linear receivers in two different asymptotic regimes. For fixed number of antennas, we investigate the limit of error probability in the high-SNR regime in terms of the Diversity-Multiplexing Tradeoff (DMT). Following this, we characterize the error probability for fixed SNR in the regime of large (but finite) number of antennas. As far as the DMT is concerned, we report a negative result: we show that both linear Zero-Forcing (ZF) and linear Minimum Mean-Square Error (MMSE) receivers achieve the same DMT, which is largely suboptimal even in the case where outer coding and decoding is performed across the antennas. We also provide an approximate quantitative analysis of the markedly different behavior of the MMSE and ZF receivers at finite rate and non-asymptotic SNR, and show that while the ZF receiver achieves poor diversity at any finite rate, the MMSE receiver error curve slope flattens out progressively, as the coding rate increases. When SNR is fixed and the number of antennas becomes large, we show that the mutual information at the output of a MMSE or ZF linear receiver has fluctuations that converge in distribution to a Gaussian random variable, whose mean and variance can be characterized in closed form. This analysis extends to the linear receiver case a well-known result previously obtained for the optimal receiver. Simulations reveal that the asymptotic analysis captures accurately the outage behavior of systems even with a moderate number of antennas.Comment: 48 pages, Submitted to IEEE Transactions on Information Theor

Maleimido substituted aromatic cyclotriphosphazenes

4-Aminophenoxy cyclotriphosphazenes are reacted with maleic anhydride to produce maleamic acids which are converted to the maleimides. The maleimides are polymerized. By selection of starting materials (e.g., hexakis amino or trisaminophenoxy-trisphenoxy-cyclo-triphosphazenes), selection of molar proportions of reactants, use of mixtures of anhydrides and use of dianhydrides as bridging groups a variety of maleimides and polymers are produced. The polymers have high limiting oxygen indices, high char yields and other useful heat and fire resistant properties making them useful as, for example, impregnants of fabrics

Toolboxes and handing students a hammer: The effects of cueing and instruction on getting students to think critically

Developing critical thinking skills is a common goal of an undergraduate physics curriculum. How do students make sense of evidence and what do they do with it? In this study, we evaluated students' critical thinking behaviors through their written notebooks in an introductory physics laboratory course. We compared student behaviors in the Structured Quantitative Inquiry Labs (SQILabs) curriculum to a control group and evaluated the fragility of these behaviors through procedural cueing. We found that the SQILabs were generally effective at improving the quality of students' reasoning about data and making decisions from data. These improvements in reasoning and sensemaking were thwarted, however, by a procedural cue. We describe these changes in behavior through the lens of epistemological frames and task orientation, invoked by the instructional moves