Половые отличия пула свободных аминокислот-нейромедиаторов у крыс Крушинского-Молодкиной

The study of the role of neurotransmitter systems in the pathogenesis of epilepsy is one of the priorities of epileptology. New data on the functions of free neurotransmitter-like amino acid in the central nervous system are of the greatest importance and determine the prospects for the development of novel effective anticonvulsants. It is widely believed in clinical medicine that epilepsy has distinct gender characteristics. The aim of this study was to investigate the gender peculiarities in the content of neurotransmitter amino acids in the brain of Krushinsky-Molodkina (KM) rats, which were used as model organisms for the study of genetically induced audiogenic epilepsy. The content of Asp, Glu, GABA, Gly, and Tau of the medulla oblongata, hippocampus and cerebral cortex were determined using high-performance liquid chromatography (HPLC) in intact KM rats, KM rats exposed to a series of epileptiform seizures, and Wistar rats (control group). Both the Wistar and KM rats had gender distinctions in the distribution of free amino acids among the investigated brain parts. The audiogenic epilepsy was characterized by smoothing gender differences as well as differences between the concentrations of free amino acids in the cortex and medulla oblongata, specific for Wistar rats. The changes observed in male rats after the set of seizures included the increase in GABA concentration and a decrease in the Gly level in all investigated brain parts, as well as the decrease of the Tau content in the cortex and hippocampus. At the same time, the Glu content in cortex increased, while the Asp level decreased. After 6 days of audiogenic stimulations the female KM rats demonstrated the increase in the Glu level in all investigated brain parts, the increase in Gly and Asp levels in hippocampus, and no changes in the GABA content. Thus, after the set of epileptiform seizures the KM rats achieved a new steady state of the studied amino acids pool, which differed in males and females. In this case, gender differences significantly changed after the seizures. © 2020 Russian Academy of Medical Sciences. All rights reserved.The work was performed within the framework of the state task of the IIF UB RAS (Registration number AAAA-A18-118020590108-7)

Coronaviruses in rodents and insectivores in Altai Republic

Coronaviruses (family Coronaviridae, genera Alphacoronavirus, Betacoronavirus) are dangerous viral pathogens that have caused three outbreaks of severe respiratory diseases, SARS, MERS, COVID-19. In Russia, data on coronaviruses in natural reservoirs are limited, as investigations began only during the COVID-19 pandemic.The aim. To study the diversity of coronaviruses among rodents and insectivores in the Republic of Altai.Materials and methods. Rodents (n  =  67) and shrews (n  =  52) were captured in 2022. Samples were analyzed by reverse transcription-polymerase chain reaction followed by sequencing.Results and conclusions. Four samples from rodents (Myodes rutilus, M. glareolus, Apodemus peninsulae, A. agrarius) and two samples from an insectivore (Crocidura sibirica) were positive for coronaviruses, among which three different coronaviruses were detected. Rodent-borne coronaviruses are classified in the genus Betacoronavirus, subgenera Embecovirus, and have shown host associated clustering. The nucleotide sequences of Siberian coronaviruses from rodents were identical for closely related species (M. rutilus and M. glareolus, A. agrarius and A. peninsulae) and close (> 94 % homology) to previously published sequences in each of the groups of carriers found in the territory Novosibirsk region, Europe and China. The coronavirus identified from the insectivore, possibly belonging to a new subgenera of the family Coronaviridae, has also been assigned to the genus Betacoronavirus.Conclusion. Five species of natural carriers of three different coronaviruses were detected in the Altai Republic. A high level of identity of coronaviruses genomes from rodents has been revealed, indicating a relatively low rate of their evolution

Silver(I) coordination compounds with polydentate pyrimidine ligands: thermochromism of luminescence

The main goal of this work is to study how the substituent in position 2 of the pyrimidine ring affects both luminescent properties and structures of silver(I) complexes. A series of coordination compounds, [AgL1NO3], [AgL1X]n, [Ag2(L2)2X2] (X = I, Br, Cl–), [AgL1(PPh3)Y] (Y = CF3SO3, BF4, NO3), [AgL3NO3·CH3CN]n, have been synthesized and structurally characterized. According to the X-ray single data, the complexes [AgL1NO3], [AgL1(PPh3)Y] adopt mononuclear structures; the complexes [Ag2(L2)2X2] are binuclear; [AgL1X]n and [AgL3NO3·CH3CN]n are polymeric compounds.The research was supported by the President of the Russian Federation Grant for young scientists МК-1219.2020.3; by Russian Foundation for Basic Research and the government of the Novosibirsk region of the Russian Federation, grant № 18–43–540016 r_a

Temperature and excitation wavelength dependent emission of silver(I) complexes with an aminopyrimidine ligand

The research was supported by the President of the Russian Federation Grant for young scientists МК-1219.2020.3; by Russian Foundation for Basic Research and the government of the Novosibirsk region of the Russian Federation, grant № 18–43–540016 r_a

Treatment-and-prophylactic means of intimate hygiene femivit in correction of some inflammatory diseases of bodies of the small basin

The experimental clinical material of therapy by means of intimate hygiene of "Femivit" on an immunity condition at patients with inflammatory diseases of bodies of a small basin is presented. The obtained data confirm that immunological indicators change towards restoration of normal values.Цель работы - оценить динамическую вариабельность некоторых показателей гуморального иммунитета при использовании средства интимной гигиены “Фемивит” в коррекции заболеваний органов малого таза

The control methods and technologies of microbiological synthesis of the complex probiotic preparation

This article is devoted to development of technologies optimizing receiving and control methods of manufacturing of complex probiotic preparationsЦель работы - оценить особенности технологии получения и определить контролирующие методы получения комплексного пробиотического препарата

ВЛИЯНИЕ РАЗЛИЧНЫХ ВИДОВ ОБРАБОТКИ НА СТРУКТУРУ, ПЛОТНОСТЬ И ЭЛЕКТРОПРОВОДНОСТЬ ДЕФОРМИРУЕМЫХ СПЛАВОВ СИСТЕМЫ Al–Mg

A comparative study was carried out to determine the effect of AlZr4, AlZr10 and AlSc2 modifying master-alloys and magnetic-pulse treatment (MPT) on the density (in liquid and solid state), electrical conductivity (in solid state) and macrostructure of AMg5 and AMg6 alloys. Molten master alloys were poured into special devices to ensure a cooling rate of ~102, ~103 and ~106 °C/s during crystallization. Master alloys were introduced into the melts at the rate of 0.01% per modifier element. It is shown that melt modification with nucleating additives contributes to higher alloy density in liquid and solid states. The electrical conductivity of the alloys with AlZr4 and AlZr10 master alloys added is lower. The AlSc2 master alloy introduction causes an increase in the electrical conductivity of AMg5 and AMg6 alloys. This effect is observed for the first time and requires additional research. It is found that the AlSc2 master alloy obtained by crystallization in a water-cooled roller mold has the greatest influence on the physical properties of alloys in comparison with AlZr4 and AlZr10 master alloys. It also provides maximum macrograin refinement. Magnetic-pulse treatment (MPT) of melts following the axial scheme of impact contributes to higher density of alloys in liquid and solid states similar to the introduction of modifying master alloys. Electrical conductivity increases after MPT as with the AlSc2 master alloy added to melts. Alloy macrograin refinement at magnetic-pulse treatment is comparable with the addition of the AlZr4 modifying master alloy. The comparative studies allow for the conclusion that magnetic-pulse treatment can be attributed to physical modification methods. The methods of density and electrical conductivity determination can be used for express evaluation of the studied effects for their modifying effectiveness.  Выполнено сравнительное исследование по влиянию модифицирующих лигатур AlZr4, AlZr10 и AlSc2, а также магнит-но-импульсной обработки (МИО) на плотность (в жидком и твердом состояниях), электропроводность (в твердом со-стоянии) и макроструктуру сплавов АМг5 и АМг6. Лигатурные расплавы заливались в специальные устройства, обеспечивающие скорости охлаждения при кристаллизации ~102, ~103 и ~106 °С/с. Лигатуры вводились в расплавы из расчета 0,01% по элементу-модификатору. Показано, что модифицирующая обработка расплавов добавками зародышеобразующих лигатур способствует повышению плотности сплавов в жидком и твердом состояниях. Электропроводность сплавов с добавками лигатур AlZr4 и AlZr10 снижается. Введение лигатуры AlSc2 вызывает повышение электропроводности сплавов АМг5 и АМг6. Данный эффект установлен впервые и требует дополнительных исследований. Установлено, что по сравнению с лигатурами AlZr4 и AlZr10 наибольшее влияние на физические свойства сплавов оказывает лигатура AlSc2, полученная кристаллизацией в водоохлаждаемом валковом кристаллизаторе. Также она обеспечивает максимальное измельчение макрозерна. Магнитно-импульсная обработка расплавов по осевой схеме воздействия, как и введение модифицирующих лигатур, способствуют увеличению плотности сплавов в жидком и твердом состояниях. Электропроводность после МИО повышается, как и после добавки в расплавы лигатуры AlSc2. Измельчение макрозерна сплавов при МИО сопоставимо с модифицированием лигатурой AlZr4. На основании сравнительных исследований сделан вывод о том, что МИО можно отнести к физическим способам модифицирования. Методики определения плотности и электропроводности предлагается использовать для экспрессной оценки модифицирующей эффективности исследованных воздействий. 

Synthesis of carboxylated derivatives of poly(isobutylene-co-isoprene) by azide–alkyne “click” chemistry

The final publication is available at Springer via https://dx.doi.org/10.1038/s41428-018-0130-yThe synthesis of carboxylated derivatives of poly(isobutylene-co-isoprene) (isobutylene–isoprene rubber, IIR) with substitution levels ranging from 1 to 4 mol% and different spacer lengths was accomplished through azide–alkyne Huisgen cycloaddition. Azido-functionalized IIR was first prepared by reacting brominated IIR with sodium azide to full conversion in a 90:10 tetrahydrofuran/N,N-dimethylacetamide mixture. The click reaction of azido-functionalized IIR with acetylenic acids, which was carried out using the copper(I) bromide/N,N,N′,N″,N″-pentamethyldiethylenetriamine catalyst system in tetrahydrofuran, yielded carboxylated IIRs. The products were characterized by 1H NMR and FT-IR spectroscopy, and their molecular weight was determined by size exclusion chromatography analysis. The conversion to carboxylated groups reached up to 100% as determined by NMR spectroscopy but was highly dependent on the type of solvent and the amounts of catalysts and reactants used in the procedures.ARLANXEO Canada Inc.Natural Sciences and Engineering Research Council (NSERC) of Canad

NEW PROSPECTS FOR LOCAL ETIOTROPIC TREATMENT AND PREVENTION OF ACUTE RESPIRATORY VIRAL INFECTIONS

According to the WHO, respiratory diseases today are among the ten most common diseases globally. Respiratory diseases of the  upper respiratory tract account for up to  60% in the  overall respiratory pathology. [1]  In addition to  antiviral and immunostimulatory drugs which shape the current strategy for the treatment of influenza and acute respiratory viral infections, the key role also belongs to symptomatic therapy. [8] Substantial positive experience with silver-based medications confirmed by numerous clinical studies demonstrates their high efficacy and satisfactory safety profile in the treatment of infectious and inflammatory diseases of the nose and upper respiratory tract in children and adults