Histological assessment of lung sections from PBS or GC frass exposed C57Bl/6 mice

<p><b>Copyright information:</b></p><p>Taken from "Differences in susceptibility to German cockroach frass and its associated proteases in induced allergic inflammation in mice"</p><p>http://respiratory-research.com/content/8/1/91</p><p>Respiratory Research 2007;8(1):91-91.</p><p>Published online 8 Dec 2007</p><p>PMCID:PMC2222603.</p><p></p> Haematoxylin and eosin (H&E) staining of sectioned lungs from PBS (A) and GC frass (B) treated C57Bl/6 mice. Periodic Acid Schiff (PAS) staining of sectioned lungs from PBS (C) and GC frass (D) treated C57Bl/6 mice. Representative slides are shown of sections from 6–7 mice per group

Histological assessment of lung sections from Balb/c mice exposed to GC frass or protease-depleted GC frass

<p><b>Copyright information:</b></p><p>Taken from "Differences in susceptibility to German cockroach frass and its associated proteases in induced allergic inflammation in mice"</p><p>http://respiratory-research.com/content/8/1/91</p><p>Respiratory Research 2007;8(1):91-91.</p><p>Published online 8 Dec 2007</p><p>PMCID:PMC2222603.</p><p></p> Periodic Acid Schiff (PAS) staining of sectioned lungs from GC frass (A) and aprotinin-treated GC frass (D) treated Balb/c mice. Representative slides are shown of sections from 8 mice per group

GC frass serine proteases regulate airway inflammation and airway hyperresponsiveness in Balb/c mice

<p><b>Copyright information:</b></p><p>Taken from "Differences in susceptibility to German cockroach frass and its associated proteases in induced allergic inflammation in mice"</p><p>http://respiratory-research.com/content/8/1/91</p><p>Respiratory Research 2007;8(1):91-91.</p><p>Published online 8 Dec 2007</p><p>PMCID:PMC2222603.</p><p></p> Balb/c mice were challenged on day 0, 7, and 14 with PBS, PBS pretreated with aprotinin (10 μg/ml), GC frass (40 μg) or GC frass pretreated with aprotinin. On day 17, mice were anesthetized and acetylcholine was injected after establishment of a stable airway pressure. A. AHR was measured as airway pressure time index (APTI) in cm-HO × sec (* p < 0.001). B. Lungs from the mice were excised, and cells dissociated and maintained in a single suspension culture for 3 days in the presence of Con A (10 μg/ml). Supernatants were removed and ELISAs were run for IL-5, IL-13 and IFNγ. These data are represented as cytokine (listed on the x-axis) in ng/ml from PBS or frass treated mice. C. Serum IgE levels were analyzed by ELISA (*p < 0.001). In all cases the data are expressed as mean ± SEM and represent 13–14 mice per group and statistical significance determined by ANOVA