<p><b>Copyright information:</b></p><p>Taken from "Differences in susceptibility to German cockroach frass and its associated proteases in induced allergic inflammation in mice"</p><p>http://respiratory-research.com/content/8/1/91</p><p>Respiratory Research 2007;8(1):91-91.</p><p>Published online 8 Dec 2007</p><p>PMCID:PMC2222603.</p><p></p> Balb/c mice were challenged on day 0, 7, and 14 with PBS, PBS pretreated with aprotinin (10 μg/ml), GC frass (40 μg) or GC frass pretreated with aprotinin. On day 17, mice were anesthetized and acetylcholine was injected after establishment of a stable airway pressure. A. AHR was measured as airway pressure time index (APTI) in cm-HO × sec (* p < 0.001). B. Lungs from the mice were excised, and cells dissociated and maintained in a single suspension culture for 3 days in the presence of Con A (10 μg/ml). Supernatants were removed and ELISAs were run for IL-5, IL-13 and IFNγ. These data are represented as cytokine (listed on the x-axis) in ng/ml from PBS or frass treated mice. C. Serum IgE levels were analyzed by ELISA (*p < 0.001). In all cases the data are expressed as mean ± SEM and represent 13–14 mice per group and statistical significance determined by ANOVA
