How nest translocation-time, clutch size and presence of yolkless eggs affected hatching success in <i>Dermochelys coriacea</i> (Linnaeus, 1766) (Testudines: dermochelyidae), at Projeto Tamar-Ibama, Espirito Santo, Brazil

En este estudio se analizó el manejo de los nidos de la especie Dermochelys coriacea, utilizados por el Proyecto Tamar-Ibama entre las temporadas reproductivas de 1989/1990 a 1998/1999, para verificación del éxito de la eclosión en relación a los tiempos de traslación. El estudio se realizó en el litoral norte del Estado de Espírito Santo. Existió tendencia a que el tiempo de traslación influencie el éxito de eclosión de los nidos. Los nidos trasladados entre 6 y más de 24 horas presentaron mayor cantidad de huevos sin desarrollo embrionario. No se encontró relación entre el número de huevos inviables trasladados y el tamaño de la postura, con el porcentual de eclosión. Se sugiere que la traslación sea realizada hasta las 6 horas, o 15 días después de la oviposición.The results of managing Dermochelys coriacea (Linnaeus, 1766) nests by Projeto TAMAR-IBAMA, in Northern Espírito Santo, Brazil, during the nesting seasons from 1989/90 to 1998/99 are analyzed. The influence of the translocation time on hatching success of the studied nests is discussed. The time translocation, in relation to natural oviposition, seems to increase the number of non-developed eggs found in each nest if translocated between 6 hours and 15 days post egg-laying. There was no relationship established between either the number of yolkless eggs in a translocated nest or the clutch size, with hatching success. The translocation of Dermochelys nests either within 6 hours or after 15 days from natural oviposition is recommended.Asociación Herpetológica Argentina (AHA

How nest translocation-time, clutch size and presence of yolkless eggs affected hatching success in <i>Dermochelys coriacea</i> (Linnaeus, 1766) (Testudines: dermochelyidae), at Projeto Tamar-Ibama, Espirito Santo, Brazil

En este estudio se analizó el manejo de los nidos de la especie Dermochelys coriacea, utilizados por el Proyecto Tamar-Ibama entre las temporadas reproductivas de 1989/1990 a 1998/1999, para verificación del éxito de la eclosión en relación a los tiempos de traslación. El estudio se realizó en el litoral norte del Estado de Espírito Santo. Existió tendencia a que el tiempo de traslación influencie el éxito de eclosión de los nidos. Los nidos trasladados entre 6 y más de 24 horas presentaron mayor cantidad de huevos sin desarrollo embrionario. No se encontró relación entre el número de huevos inviables trasladados y el tamaño de la postura, con el porcentual de eclosión. Se sugiere que la traslación sea realizada hasta las 6 horas, o 15 días después de la oviposición.The results of managing Dermochelys coriacea (Linnaeus, 1766) nests by Projeto TAMAR-IBAMA, in Northern Espírito Santo, Brazil, during the nesting seasons from 1989/90 to 1998/99 are analyzed. The influence of the translocation time on hatching success of the studied nests is discussed. The time translocation, in relation to natural oviposition, seems to increase the number of non-developed eggs found in each nest if translocated between 6 hours and 15 days post egg-laying. There was no relationship established between either the number of yolkless eggs in a translocated nest or the clutch size, with hatching success. The translocation of Dermochelys nests either within 6 hours or after 15 days from natural oviposition is recommended.Asociación Herpetológica Argentina (AHA

As conseqüências do manejo sobre os ninhos de <i>Dermochelys coriacea</i> (Linnaeus, 1766), junto ao projeto Tamar-Ibama, Espirito Santo, Brasil

Foi analisada a viabilidade dos manejos de ninhos utilizados pelo Projeto Tamar-Ibama, para a esp écie Dermochelys coriacea nas temporadas reprodutivas entre 1989/90 e 1998/99. Este estudo foi realizado no litoral norte do Estado do Espírito Santo. As desovas foram manejadas de três diferentes formas: mantidas In Situ, transferidas para locais na praia e, ainda, para cercados de incubação. As análises realizadas mostraram que os ninhos que permaneceram In Situ obtiveram os melhores resultados em termos de percentuais de eclosão e números de embriões. Entre os ninhos transferidos, os que foram para os cercados de incubação geraram maior número de filhotes vivos e reduzido número de embriões grandes e pequenos. O único fator que não foi influenciado pela transferência dos ovos, foi o número de natimortos. Foi encontrada diferença significativa no tempo de incubação dos ninhos entre os manejos aplicados. É sugerida a manutenção de uma maior quantidade de ninhos In Situ, e, a criação de Unidades de Conservação em áreas com pouco desenvolvimento urbano e alta concentração de desovas.The analysis was made to compare the viability of the management of nests utilized by Projeto Tamar-Ibama, for the species Dermochelys coriacea in the nesting seasons between 1989/90 and 1998/99. This study was made in northern Espírito Santo. The clutches were managed in three different ways: kept In Situ, translocated to beaches, or to hatcheries. The analyzes showed that In Situ nests had the best results in terms of hatching success and number of embryos. Among the translocated nests, those that went to the hatcheries reached the best results in numbers of hatchlings and small and medium embryos. The only factor that was not influenced by the translocation was the number of piped embryos. A significant difference was found between the hatching period and management used. It is suggested to keep the nest in their natural places (In Situ) as much as possible and the implement of the natural reserves where there is little urban development and a high number of clutches.Asociación Herpetológica Argentina (AHA

As conseqüências do manejo sobre os ninhos de <i>Dermochelys coriacea</i> (Linnaeus, 1766), junto ao projeto Tamar-Ibama, Espirito Santo, Brasil

Foi analisada a viabilidade dos manejos de ninhos utilizados pelo Projeto Tamar-Ibama, para a esp écie Dermochelys coriacea nas temporadas reprodutivas entre 1989/90 e 1998/99. Este estudo foi realizado no litoral norte do Estado do Espírito Santo. As desovas foram manejadas de três diferentes formas: mantidas In Situ, transferidas para locais na praia e, ainda, para cercados de incubação. As análises realizadas mostraram que os ninhos que permaneceram In Situ obtiveram os melhores resultados em termos de percentuais de eclosão e números de embriões. Entre os ninhos transferidos, os que foram para os cercados de incubação geraram maior número de filhotes vivos e reduzido número de embriões grandes e pequenos. O único fator que não foi influenciado pela transferência dos ovos, foi o número de natimortos. Foi encontrada diferença significativa no tempo de incubação dos ninhos entre os manejos aplicados. É sugerida a manutenção de uma maior quantidade de ninhos In Situ, e, a criação de Unidades de Conservação em áreas com pouco desenvolvimento urbano e alta concentração de desovas.The analysis was made to compare the viability of the management of nests utilized by Projeto Tamar-Ibama, for the species Dermochelys coriacea in the nesting seasons between 1989/90 and 1998/99. This study was made in northern Espírito Santo. The clutches were managed in three different ways: kept In Situ, translocated to beaches, or to hatcheries. The analyzes showed that In Situ nests had the best results in terms of hatching success and number of embryos. Among the translocated nests, those that went to the hatcheries reached the best results in numbers of hatchlings and small and medium embryos. The only factor that was not influenced by the translocation was the number of piped embryos. A significant difference was found between the hatching period and management used. It is suggested to keep the nest in their natural places (In Situ) as much as possible and the implement of the natural reserves where there is little urban development and a high number of clutches.Asociación Herpetológica Argentina (AHA

Dieta e comportamento alimentar da Cobra-nariguda listrophis dorbignyi (Dumeril, Bibrón & Duméril, 1854), no litoral norte do Rio Grande do Sul, Brasil

Os dados aqui apresentados são fundamentados em 214 encontros de indivíduos de Lystrophis dorbignyi em uma área de dunas do Litoral Norte do Rio Grande do Sul, e na análise do tubo digestório de 33 espécimes procedentes da mesma região. Anfíbios anuros e ovos da lagartixa Liolaemus occipitalis foram os únicos itens alimentares registrados. Entre as serpentes observadas com presas, 94,5 % se alimentaram de anfíbios e 5,6 % se alimentaram de ovos de L. occipitalis. Os períodos de atividade e os microhábitats das espécies de anfíbios mais freqüentemente consumidas, a inclusão de ovos na dieta e a observação de sete indivíduos de L. dorbignyi se alimentando em condições naturais, indicam que esta serpente forrageia durante o dia e captura presas enterradas na areia. Alguns itens sugeridos por vários autores como presas de L. dorbignyi são descartados como tal, até que dados consistentes sejam apresentados.The data here presented are based on 214 sightings of individuals of the snake Lystrophis dorbignyi in an area of sand dunes in the north coastal plain of Rio Grande do Sul, and on the analysis of the stomach and gut contents of 33 specimens proceeding from this region. Only anurans and eggs of the lizard Liolaemus occipitalis were recorded. Among the snakes with food items, 94,5% had anurans and 5,6% had eggs of L. occipitalis. The recorded period of activity, the microhabitats of the items most frequently consumed, the addition of eggs in the diet, and the observation of seven individuals of L. dorbignyi taking preys under natural conditions, indicate that this snake forages during the day, and catches preys burrowed in the sand. Some food items suggested by many authors as being preys of L. dorbignyi are here discarded, until more consistent data were presented.Asociación Herpetológica Argentin

DNA-Methylome based Tumor Hypoxia Classifier Identifies HPV-negative Head & Neck Cancer Patients at Risk for Locoregional Recurrence After Primary Radiochemotherapy

BACKGROUND Tumor hypoxia is a paradigmatic negative prognosticator of treatment resistance in Head and Neck Squamous Cell Carcinoma (HNSCC). The lack of robust and reliable hypoxia classifiers limits the adaptation of stratified therapies. We hypothesized that the tumor DNA methylation landscape might indicate epigenetic reprogramming induced by chronic intratumoral hypoxia. METHODS A DNA methylome-based tumor hypoxia classifier (Hypoxia-M) was trained in the TCGA-HNSCC cohort based on matched assignments using gene expression-based signatures of hypoxia (Hypoxia-GES). Hypoxia-M was validated in a multicenter DKTK-ROG trial consisting of Human Papilloma Virus (HPV)-negative HNSCC patients treated with primary radiochemotherapy (RCHT). RESULTS While hypoxia-GSEs failed to stratify patients in the DKTK-ROG, Hypoxia-M was independently prognostic for local recurrence (LR, HR=4.3, p=0.001) and overall survival (OS, HR=2.34, p=0.03) but not distant metastasis (DM) after RCHT in the both cohorts. Hypoxia-M status was inversely associated with CD8 T-cells infiltration in both cohorts. Hypoxia-M was further prognostic in the TCGA-PanCancer cohort (HR=1.83, p=0.04), underscoring the breadth of this classifier for predicting tumor hypoxia status. CONCLUSIONS Our findings highlight an unexplored avenue for DNA Methylation-based classifiers as biomarkers of tumoral hypoxia for identifying high-risk features in patients with HNSCC tumors. TRIAL REGISTRATION Retrospective observational study from the German Cancer Consortium (DKTK-ROG), not interventional

Genome of Herbaspirillum seropedicae Strain SmR1, a Specialized Diazotrophic Endophyte of Tropical Grasses

The molecular mechanisms of plant recognition, colonization, and nutrient exchange between diazotrophic endophytes and plants are scarcely known. Herbaspirillum seropedicae is an endophytic bacterium capable of colonizing intercellular spaces of grasses such as rice and sugar cane. The genome of H. seropedicae strain SmR1 was sequenced and annotated by The Paraná State Genome Programme—GENOPAR. The genome is composed of a circular chromosome of 5,513,887 bp and contains a total of 4,804 genes. The genome sequence revealed that H. seropedicae is a highly versatile microorganism with capacity to metabolize a wide range of carbon and nitrogen sources and with possession of four distinct terminal oxidases. The genome contains a multitude of protein secretion systems, including type I, type II, type III, type V, and type VI secretion systems, and type IV pili, suggesting a high potential to interact with host plants. H. seropedicae is able to synthesize indole acetic acid as reflected by the four IAA biosynthetic pathways present. A gene coding for ACC deaminase, which may be involved in modulating the associated plant ethylene-signaling pathway, is also present. Genes for hemagglutinins/hemolysins/adhesins were found and may play a role in plant cell surface adhesion. These features may endow H. seropedicae with the ability to establish an endophytic life-style in a large number of plant species

How nest translocation-time, clutch size and presence of yolkless eggs affected hatching success in <i>Dermochelys coriacea</i> (Linnaeus, 1766) (Testudines: dermochelyidae), at Projeto Tamar-Ibama, Espirito Santo, Brazil

En este estudio se analizó el manejo de los nidos de la especie Dermochelys coriacea, utilizados por el Proyecto Tamar-Ibama entre las temporadas reproductivas de 1989/1990 a 1998/1999, para verificación del éxito de la eclosión en relación a los tiempos de traslación. El estudio se realizó en el litoral norte del Estado de Espírito Santo. Existió tendencia a que el tiempo de traslación influencie el éxito de eclosión de los nidos. Los nidos trasladados entre 6 y más de 24 horas presentaron mayor cantidad de huevos sin desarrollo embrionario. No se encontró relación entre el número de huevos inviables trasladados y el tamaño de la postura, con el porcentual de eclosión. Se sugiere que la traslación sea realizada hasta las 6 horas, o 15 días después de la oviposición.The results of managing Dermochelys coriacea (Linnaeus, 1766) nests by Projeto TAMAR-IBAMA, in Northern Espírito Santo, Brazil, during the nesting seasons from 1989/90 to 1998/99 are analyzed. The influence of the translocation time on hatching success of the studied nests is discussed. The time translocation, in relation to natural oviposition, seems to increase the number of non-developed eggs found in each nest if translocated between 6 hours and 15 days post egg-laying. There was no relationship established between either the number of yolkless eggs in a translocated nest or the clutch size, with hatching success. The translocation of Dermochelys nests either within 6 hours or after 15 days from natural oviposition is recommended.Asociación Herpetológica Argentina (AHA

As conseqüências do manejo sobre os ninhos de Dermochelys coriacea (Linnaeus, 1766), junto ao projeto Tamar-Ibama, Espírito Santo, Brasil

Foi analisada a viabilidade dos manejos de ninhos utilizados pelo Projeto Tamar-Ibama, para a esp écie Dermochelys coriacea nas temporadas reprodutivas entre 1989/90 e 1998/99. Este estudo foi realizado no litoral norte do Estado do Espírito Santo. As desovas foram manejadas de três diferentes formas: mantidas In Situ, transferidas para locais na praia e, ainda, para cercados de incubação. As análises realizadas mostraram que os ninhos que permaneceram In Situ obtiveram os melhores resultados em termos de percentuais de eclosão e números de embriões. Entre os ninhos transferidos, os que foram para os cercados de incubação geraram maior número de filhotes vivos e reduzido número de embriões grandes e pequenos. O único fator que não foi influenciado pela transferência dos ovos, foi o número de natimortos. Foi encontrada diferença significativa no tempo de incubação dos ninhos entre os manejos aplicados. É sugerida a manutenção de uma maior quantidade de ninhos In Situ, e, a criação de Unidades de Conservação em áreas com pouco desenvolvimento urbano e alta concentração de desovas. <br> The analysis was made to compare the viability of the management of nests utilized by Projeto Tamar-Ibama, for the species Dermochelys coriacea in the nesting seasons between 1989/90 and 1998/99. This study was made in northern Espírito Santo. The clutches were managed in three different ways: kept In Situ, translocated to beaches, or to hatcheries. The analyzes showed that In Situ nests had the best results in terms of hatching success and number of embryos. Among the translocated nests, those that went to the hatcheries reached the best results in numbers of hatchlings and small and medium embryos. The only factor that was not influenced by the translocation was the number of piped embryos. A significant difference was found between the hatching period and management used. It is suggested to keep the nest in their natural places (In Situ) as much as possible and the implement of the natural reserves where there is little urban development and a high number of clutches