Синтез и исследование алюмината стронция Sr3Al2O6 активированного ионами европия

Алюминат стронция был получен методом золь-гель. Использовали термическую обработку в сушильном шкафу для сушки геля. Для получения кристаллического продукта кубической модификации все образцы прокаливали в муфельной печи при температурах до 1000 ° С. Морфологию поверхности прекурсора исследовали при растрового электронного микроскопа Hitachi ТМЗООО. На основании данных рентгенофазового анализа сделали вывод об образовании алюмината стронция состава SrsAl20g с незначительными примесями фазы SrAl^)j. Золь-гель методом был получен люминофор состава 8г2_8Еио_2А1Д>б- Экспериментально было подтверждено свечение красного цвета полученного люминофора

Fabrication of Magnetically Responsive Agarose Microbeads Doped with Live Microbial Cells

© 2016, Springer Science+Business Media New York.Here, we report a scalable and rapid method to fabricate magnetically responsive agarose microgels doped with microbial cells. Low-temperature melting agarose and food-grade sunflower oil were used to fabricate microbeads during emulsification and gel setting. Microscopic algae and fungi cells were doped into ∼100-μm-sized beads as single culture or mixed. Magnetic nanoparticles were deposited either on cell walls or on bead walls. We found that the cells encapsulated in magnetically responsive microbeads were viable and able for germination

Nanoshell Assembly for Magnet-Responsive Oil-Degrading Bacteria

© 2016 American Chemical Society.The modified polyelectrolyte-magnetite nanocoating was applied to functionalize the cell walls of oil decomposing bacteria Alcanivorax borkumensis. Cationic coacervate of poly(allylamine) and 20 nm iron oxide nanoparticles allowed for a rapid single-step encapsulation process exploiting electrostatic interaction with bacteria surfaces. The bacteria were covered with rough 70-100-nm-thick shells of magnetite loosely bound to the surface through polycations. This encapsulation allowed for external manipulations of A. borkumensis with magnetic field, as demonstrated by magnetically facilitated cell displacement on the agar substrate. Magnetic coating was naturally removed after multiple cell proliferations providing next generations of the cell in the native nonmagnetic form. The discharged biosurfactant vesicles indicating the bacterial functionality (150 ± 50 nm lipid micelles) were visualized with atomic force microscopy in the bacterial biofilms

Magnetic coiffure: Engineering of human hair surfaces with polyelectrolyte-stabilised magnetite nanoparticles

Here we report a spontaneous electrostatic coating of human hair with aqueous Fe3O4 colloids capable to tailor magnetic properties to hair, orienting and even moving them under the influence of the external magnetic field. Magnetite particles were modified by cationic and anionic polyelectrolytes and then successfully deposited in dense arrays, starting from cuticle gaps and spreading further over a major hair surface. These biocompatible and biodegradable magnetic nanoparticles may serve as carriers for drug loading and delivery for topical pharmaceutical treatments. The deposition process was imaged in real-time using dark-field microscopy. The hair specimens were further studied using a number of characterisation techniques. Under application of an external magnetic field, the nanoparticle magnetic ordering was obtained resulting in the hair alignment and attraction along the field applied. We believe the technology reported here will find a range of applications in topical drug delivery and hair care

Magnetic halloysite nanotubes for yeast cell surface engineering

© 2016 The Mineralogical Society.Halloysite clay nanotubes are safe and biocompatible nanomaterials and their application in biomaterials is very promising. The microencapsulation of yeast cells in the shell of clay nanotubes modifying their properties was demonstrated here. Each cell was coated with a 200-300 nm-thick tube shell and this coating was not harmful for these cells' reproduction. Synthesis of magnetic nanoparticles on the surfaces of the nanotubes allowed for magnetic-field manipulation of the coated cells, including their separation. Providing nano-designed shells for biological cells is a step forward in development of 'cyborg' microorganisms combining their intrinsic properties with functions added through nano-engineering

Cyborg cells: Functionalisation of living cells with polymers and nanomaterials

Living cells interfaced with a range of polyelectrolyte coatings, magnetic and noble metal nanoparticles, hard mineral shells and other complex nanomaterials can perform functions often completely different from their original specialisation. Such "cyborg cells" are already finding a range of novel applications in areas like whole cell biosensors, bioelectronics, toxicity microscreening, tissue engineering, cell implant protection and bioanalytical chemistry. In this tutorial review, we describe the development of novel methods for functionalisation of cells with polymers and nanoparticles and comment on future advances in this technology in the light of other literature approaches. We review recent studies on the cell viability and function upon direct deposition of nanoparticles, coating with polyelectrolytes, polymer assisted assembly of nanomaterials and hard shells on the cell surface. The cell toxicity issues are considered for many practical applications in terms of possible adverse effects of the deposited polymers, polyelectrolytes and nanoparticles on the cell surface. © 2012 The Royal Society of Chemistry

Functional artificial free-standing yeast biofilms

Here we report fabrication of artificial free-standing yeast biofilms built using sacrificial calcium carbonate-coated templates and layer-by-layer assembly of extracellular matrix-mimicking polyelectrolyte multilayers. The free-standing biofilms are freely floating multilayered films of oppositely charged polyelectrolytes and live cells incorporated in the polyelectrolyte layers. Such biofilms were initially formed on glass substrates of circular and ribbon-like shapes coated with thin layers of calcium carbonate microparticles. The templates were then coated with cationic and anionic polyelectrolytes to produce a supporting multilayered thin film. Then the yeast alone or mixed with various micro- and nanoparticle inclusions was deposited onto the multilayer composite films and further coated with outer polyelectrolyte multilayers. To detach the biofilms from the glass substrates the calcium carbonate layer was chemically dissolved yielding free-standing composite biofilms. These artificial biofilms to a certain degree mimic the primitive multicellular and colonial species. We have demonstrated the added functionality of the free-standing artificial biofilms containing magnetic, latex and silver micro- and nanoparticles. We have also developed "symbiotic" multicellular biofilms containing yeast and bacteria. This approach for fabrication of free-standing artificial biofilms can be potentially helpful in development of artificial colonial microorganisms composed of several different unicellular species and an important tool for growing cell cultures free of supporting substrates. © 2011 Elsevier B.V

Silver nanoparticle-coated "cyborg" microorganisms: Rapid assembly of polymer-stabilised nanoparticles on microbial cells

© The Royal Society of Chemistry. Fabrication of "cyborg" cells (biological cells with surfaces functionalised using a variety of nanomaterials) has become a fascinating area in cell surface engineering. Here we report a simple procedure for fabrication of polycation-stabilised 50 nm silver nanoparticles and application of these nanoparticles for fabrication of viable "cyborg" microbial cells (yeast and bacteria). Cationic polymer-stabilised nanoparticles electrostatically adhere to microbial cells producing an even monolayer on the cell walls, as demonstrated using enhanced dark-field microscopy, atomic force microscopy and microelectrophoresis. Our procedure is exceptionally fast, being completed within 20 min after introduction of cells into nanoparticle aqueous suspensions. Polymer-stabilised silver nanoparticles are highly biocompatible, with viability rates reaching 97%. We utilised "cyborg" cells built using bacteria and silver nanoparticles to deliver nanoparticles into C. elegans microworms. We believe that the technique described here will find numerous applications in cell surface engineering. This journal i

Nanomodified Bacteria Alcanivorax Borkumensis as an Indicator of Carbohydrates in Sea Water

The work was supported by the Russian Foundation for Basic Research (grant № 18-34-00778), by Program of Competitive Growth of KFU and funded by Russian presidential grant (MK-4498.2018.4)

Biomimetic cell-mediated three-dimensional assembly of halloysite nanotubes

Biomimetic architectural assembly of clay nanotube shells on yeast cells was demonstrated producing viable artificial hybrid inorganic-cellular structures (armoured cells). These modified cells were preserved for one generation resulting in the intact second generation of cells with delayed germination. © 2013 The Royal Society of Chemistry