City of San Luis Obispo, Open Space Survey

The City of San Luis Obispo (SLO) has eleven official public open spaces. Managing these places presents a challenge in providing top-tier environmental stewardship, while also accommodating passive recreational use and access, in an era of fiscal limitations and competing priorities. Given that reality and the changing population dynamics in the US, providing equal access to these facilities is of increasing importance, whether by car, bike, on foot or via public transit. These open spaces provide value (environmental, economic and social) primarily to residents, as well as visitors, and gaining a data-driven understanding of that value was a primary goal of this study. Within this framework, team of undergraduate and graduate students from CalPoly, San Luis Obispo worked under the direction of Dr. William Riggs and Natural Resources Manager Robert Hill, to evaluate the conditions, characteristics-of, and visitors-to SLO open spaces. To accomplish this, the project team conducted an initial facilities assessment of the existing conditions at the entrances to SLO’s open spaces. Following this, a survey was conducted to gather information about use, conditions and travel / access to local open spaces. This was complimented by use data gathered from electronic counters placed at open space entrances

San Luis Obispo Open Space Survey

The City of San Luis Obispo (SLO) has eleven official public open spaces. Managing these places presents a challenge in providing top-tier environmental stewardship, while also accommodating passive recreational use and access, in an era of fiscal limitations and competing priorities. Given that reality and the changing population dynamics in the US, providing equal access to these facilities is of increasing importance, whether by car, bike, on foot or via public transit. These open spaces provide value (environmental, economic and social) primarily to residents, as well as visitors, and gaining a data-driven understanding of that value was a primary goal of this study. Within this framework, team of undergraduate and graduate students from CalPoly, San Luis Obispo worked under the direction of Dr. William Riggs and Natural Resources Manager Robert Hill, to evaluate the conditions, characteristics-of, and visitors-to SLO open spaces. To accomplish this, the project team conducted an initial facilities assessment of the existing conditions at the entrances to SLO’s open spaces. Following this, a survey was conducted to gather information about use, conditions and travel / access to local open spaces. This was complimented by use data gathered from electronic counters placed at open space entrances

Construction, theory, and practical considerations for using self-referencing of Ca2+-selective microelectrodes for monitoring extracellular Ca2+ gradients

Author Posting. © The Author(s), 2010. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Methods in Cell Biology 99 (2010): 91-111, doi:10.1016/B978-0-12-374841-6.00004-9.Ca2+ signaling in the extra- and intracellular domains is linked to Ca2+ transport across the plasma membrane. Non-invasive monitoring of these resulting extracellular Ca2+ gradients with self-referencing of Ca2+-selective microelectrodes is used for studying Ca2+ signaling across Kingdoms. The quantitated Ca2+ flux enables comparison with changes to intracellular [Ca2+] measured with other methods and determination of Ca2+ transport stoichiometry. Here we review the construction of Ca2+-selective microelectrodes, their physical characteristics and their use in self-referencing mode to calculate Ca2+ flux. We also discuss potential complications when using them to measure Ca2+ gradients near the boundary layers of single cells and tissues.NIH:NCRR grant P41 RR00139

SJU Class of 1998 Commencement Celebration

May 24, 1998 One-Hundred Forty-First Year Abbey & University Church Saint John\u27s University Dr. Evan Maurer was the guest speaker and Lance Bodeen was the student speaker