PERIPHERAL AMELOBLASTOMA OF THE BUCCAL MUCOSA : WITH A REVIEW OF THE LITERATURE

A peripheral ameloblastoma arising on the buccal mucosa is exceedingly rare. Only three document cases have been reported, and we present one additional case of this tumor. In this case, the lesion repeatedly occurred in the same location in spite of twice local excisions. The first lesion was diagnosed as basal cell carcinoma with ameloblastomatous features. The pathological findings of the lesion were palisading of the peripheral cells of epithelial masses and structures resembling stellate reticulum, which could also be observed also in peripheral ameloblastomas. The second lesion was diagnosed as basal cell adenoma, and the third as peripheral ameloblastoma. The histogenesis of peripheral ameloblastomas and intraoral basal cell carcinomas are discussed, especially with reference to the lesions of the buccal mucosa

MALIGNANT FIBROUS HISTIOCYTOMA OF THE LUNG : A Case Report and Immunohistochemical Examination of the Case

Malignant fibrous histiocytoma (MFH) arising in the lung of an autopsy case, a 52-year-old man, is reported with immunohistochemical findings. In most areas, a storiform patterns with fibroblast-like cells and histiocyte-like cells were noted. Extensive carcinoma invasion and multiple metastases were found in many organs such as mediastinum, heart, aorta, left pleural cavity, abdominal wall, omentum, peritomeum, liver, intestine, adrenal and kidneys. The tumor was mainly composed of spindle cells and had storiform and herring-bone patterns. immunohistochemically, these tumor cells were demonstrated to possess vimentin, alpha-l-antitrypsin, alpha-l-antichymotrypsin and lysozyme

Expression of c-myc, c-fos and CA19-9 in Human Non-Malignant and Malignant Gallbladder Tissues

Immunohistochemical study was performed on expressions of c-myc, c-fos and CA19-9 in gallbladder tissue with or without malignant lesions. A total of 81 tissues were divided into four groups including 47 carcinomas, 3 dysplasias, 17 metaplasias and 14 normal lesions. After these tissues were routinely fixed in 10% formalin solution and embedded in paraffin, 4 micrometer-thick sections were made and stained with hematoxylin-eosin to classify the type of lesions. Immunohistochemical stains were carried out for c-myc and c-fos oncoproteins, and CA19-9. The percentages of positive reaction for c-myc oncoprotein were 77%, 67%, 88% and 36%, those for c-fos oncoprotein were 83%, 66%, 35% and 7%, and those for CA19-9 were 85%, 100%, 88% and 71% in carcinoma, dysplasia, metaplasia and normal tissues, respectively. These results suggest that c-myc and c-fos oncogenes play some kind of roles in malignant transformation of the gallbladder tissues and that abnormal expression of CA19-9 is the sign of antigen reversion of carcinoma cells toward embryonic cells of the gallbladder tissue

Radiation Induced Cell Death in Cervical Squamous Cell Carcinoma : An Immunohistochemical and Ultrastructural Study

To study the process of cell death in cervical squamous cell carcinoma (SCC) after radiation, an ultrastructural and immunohistochemical study was performed. Paraffinembedded tissue blocks of biopsy samples pre- and postradiation stage III SCC (n = 15) were collected. Irradiation caused varying ultrastructural changes including nuclear and cytoplasmic disorganization suggesting cell necrosis. Immunohistochemically, the pre-radiation specimens showed no positive reaction for tumor necrosis factor-alpha (TNF- ﾎｱ),tumor necrosis factor-receptor(TNF-ﾎｳ)or Fas. C-fos, p 53 and bcl-2 showed positive reactions in only a few nonirradiated specimens. All of the irradiated specimens showed a positive reaction for TNF-ﾎｱ,and variable positive reactions were observed for TNF-ﾎｳ,Fas, p 53, c-fos and bcl-2. These results suggest that TNF-ﾎｱ,TNF-ﾎｳ,and c-fos are responsible for radiation induced cell death in cervical SCC

An Autopsy Case of Pleomorphic Leiomyosarcoma arising from the Uterus

A 49-year-old female patient was referred to this hospital due to uterine tumor. Needle biopsy of the uterine body was done after admission which suggested MFH (malignant fibrous histiocytoma) or poorly differentiated adenocarcinoma. The patient died after 11 days of admission before doing any surgical treatment for the tumor. Autopsy disclosed an adult head-sized pelvic tumor mass with multiple metastasis in lung, peritoneum and intestine. We confirmed the diagnosis of the present case as pleomorphic leiomyosarcoma by immunohistochemical study and electron microscopy in addition to routine light microscopy. The DNA distribution pattern of the tumor was determined by cytofluorometry and it was aneuploidy. The case report is presented with histological, immunohistochemical, electron microscopical, and cytofluorometrical study herein

Morphological and Biochemical Evaluation of the Induction of Atherosclerosis in Japanese Quails

A total of 77 birds were divided into 7 groups which were fed the following diets : Group I, basal ; Group II, 5% corn oil (CO) + 0.5% cholesterol (CH) ; Group III, 5% CO + 2% CH; Group IV, 5% CO + 4% CH; Group V, 15% CO + 0.5% CH ; Group V I, 15% CO + 2% CH; Group VII, 15% CO + 4% CH. Significant increase of serum lipid, accumulation of lipid in the liver, and lipid-rich aortic lesions were produced in Groups IV, VI and VIII. However, hyperlipidemia correlated well with the extent of hepatic lipid accumulation and severity of aortic atherosclerosis in Group VI. Proliferating intimal cells showed positive reaction to antibodies for vimentin and alpha-1-antichymotrypsin implicating an important role of phenotypical transformation of intimal cells from the medial fibroblastic cells in the development of aortic atherosclerosis. These results suggest that Japanese quail is highly susceptible to atherosclerosis, and the optimal dietary level of cholesterol and corn oil is 2% and 15%, respectively to induce lipid-rich aortic lesions in Japanese quail

A Case Report of Malignant Mullerian Mixed Tumor of the Uterus

We experienced a case of malignant Mullerian mixed tumor of the uterus of a 58-year-old female. We performed an immunohistochemical study in order to analyze the expression of various antigens in different elements of this tumor. Keratin, carcinoembryonic antigen (CEA), and epithelial membrane antigen (EMA) showed strong positive results in epithelial component, while EMA also showed positive results in some areas of sarcomatous elements. On the other hand, vimentin and S-100 gave strong positive result in non-epithelial component, but also was positive to some extent in epithelial component in this tumor. Immunohistochemical method was useful to differentiate the different components of this tumor. However, EMA, Vimentin and S-100 showed some unusual results. This discrepancy may indicate a change in the nature of tumor cells due to `microenvironmental\u27 factors such as hormones and vita - mins. Therefore, careful evaluation is necessary to interpret the immunohistochemical results in surgical pathology

Comparative Pathological Study of Coronary Arteriosclerosis in Okinawa, Nagasaki and Akita Prefectures

In order to clarify the degree of coronary arteriosclerosis in Okinawa, we carried out comparative histological study of coronary arteriosclerosis in 418 autopsy cases, ranging from 50 to 79 of age, which were autopsied between 1983 and 1988 in Okinawa, Nagasaki and Akita districts. Coronary artery samples were collected from cases which had arteriosclerosis-related diseases such as ischemic heart diseases, cerebrovascular diseases, diabetes mellitus, renal diseases, and collagen diseases. Arteriosclerotic lesions were prevalently seen in the following order : main trunk of the left coronary artery, right coronary artery and left circumflex artery. Degree of coronary arteriosclerosis was more severe in Akita than in Nagasaki and Okinawa. There was no significant difference in the degree of coronary arteriosclerosis in Okinawa and Nagasaki. Key words : Coronary arteriosclerosis, Okinawa, Nagasaki, Akita

Expression of c-myc, c-fos and CA19-9 in Human Non-Malignant and Malignant Gallbladder Tissues

Immunohistochemical study was performed on expressions of c-myc, c-fos and CA19-9 in gallbladder tissue with or without malignant lesions. A total of 81 tissues were divided into four groups including 47 carcinomas, 3 dysplasias, 17 metaplasias and 14 normal lesions. After these tissues were routinely fixed in 10% formalin solution and embedded in paraffin, 4 micrometer-thick sections were made and stained with hematoxylin-eosin to classify the type of lesions. Immunohistochemical stains were carried out for c-myc and c-fos oncoproteins, and CA19-9. The percentages of positive reaction for c-myc oncoprotein were 77%, 67%, 88% and 36%, those for c-fos oncoprotein were 83%, 66%, 35% and 7%, and those for CA19-9 were 85%, 100%, 88% and 71% in carcinoma, dysplasia, metaplasia and normal tissues, respectively. These results suggest that c-myc and c-fos oncogenes play some kind of roles in malignant transformation of the gallbladder tissues and that abnormal expression of CA19-9 is the sign of antigen reversion of carcinoma cells toward embryonic cells of the gallbladder tissue