Genome-wide functional analysis using the barcode sequence alignment and statistical analysis (Barcas) tool

Pipelines of pooled library screen analysis. Table S1. Public library sets of shRNA, sgRNA and deletion mutant strains. Figure S2. A list of wrong barcodes from 1,230 shRNAs of TRC library. Table S2. Sequences of 25 barcodes with abnormally increased mapping counts by imperfect matching. Table S3. A list of the used options for each tool. Table S4. Comparison of mapping results and speed by three tools. (PDF 7691 kb

Agaricus blazei Extract Induces Apoptosis through ROS-Dependent JNK Activation Involving the Mitochondrial Pathway and Suppression of Constitutive NF-κB in THP-1 Cells

Agaricus blazei is widely accepted as a traditional medicinal mushroom, and it has been known to exhibit immunostimulatory and anti-cancer activity. However, the apoptotic mechanism in cancer cells is poorly understood. In this study, we have investigated whether A. blazei extract (ABE) exerts antiproliferative and apoptotic effects in human leukemic THP-1 cells. We observed that ABE-induced apoptosis is associated with the mitochondrial pathway, which is mediated by reactive oxygen species (ROS) generation and prolonged c-Jun N-terminal kinase (JNK) activation. In addition, the ABE treatment resulted in the accumulation of cytochrome c in the cytoplasm, an increase in caspase activity, and an upregulation of Bax and Bad. With those results in mind, we found that ABE decreases constitutive NF-κB activation and NF-κB-regulated gene products such as IAP-1 and -2. We concluded that ABE induces apoptosis with ROS-dependent JNK activation and constitutive activated NF-κB inhibition in THP-1 cells

Regularity for Solutions of Second-Order Nonlinear Integrodifferential Functional Equations

We deal with the well-posedness for solutions of nonlinear integrodifferential equations of second-order in Hilbert spaces by converting the problem into the contraction mapping principle with more general conditions on the principal operators and the nonlinear terms and obtain a variation of constant formula of solutions of the given nonlinear equations

A Research on the Trend Pattern Analysis of Industrial Water Consumption

The purpose of this study is to propose the average trend pattern of the industrial water consumption. A real-time sensing technique was adopted for the consumption data acquisition. Data were transformed from the field equipments to the management server in every 5 minutes. The data acquired were substituted to a polynomial formula selected. As a result, a series of models were developed for each day. Each of these models is finalized to represent the average water consumption for the day. In this way, an average consumption pattern is derived for each day of a week. As a result of the aforementioned research, the proposed trend pattern analysis technique is expected to offer some important role for the water supply forecasting administration and management

Combined Effects of Surface Morphology and Mechanical Straining Magnitudes on the Differentiation of Mesenchymal Stem Cells without Using Biochemical Reagents

Existing studies examining the control of mesenchymal stem cell (MSC) differentiation into desired cell types have used a variety of biochemical reagents such as growth factors despite possible side effects. Recently, the roles of biomimetic microphysical environments have drawn much attention in this field. We studied MSC differentiation and changes in gene expression in relation to osteoblast-like cell and smooth muscle-like cell type resulting from various microphysical environments, including differing magnitudes of tensile strain and substrate geometries for 8 days. In addition, we also investigated the residual effects of those selected microphysical environment factors on the differentiation by ceasing those factors for 3 days. The results of this study showed the effects of the strain magnitudes and surface geometries. However, the genes which are related to the same cell type showed different responses depending on the changes in strain magnitude and surface geometry. Also, different responses were observed three days after the straining was stopped. These data confirm that controlling microenvironments so that they mimic those in vivo contributes to the differentiation of MSCs into specific cell types. And duration of straining engagement was also found to play important roles along with surface geometry

Bilateral hydrothorax and cardiac tamponade after right subclavian vein catheterization -A case report-

Central venous catheterization is typically used for the anesthetic management of patients undergoing a major surgery or care of patients in Intensive Care Unit (ICU). The occurrence of complications associated with central venous catheterization such as pneumothorax or vascular injury have decreased, while delayed complications such as hydrothorax, hydromediastinum, or cardiac tamponade have risen recently. We report a case of complications of bilateral hydrothorax with cardiac tamponade by superior vena cava perforation due to continuous mechanical force of the looped central venous catheter tip against SVC wall after subclavian vein cannulation

High-Accuracy AM-FM Radar with an Active Reflector

An amplitude-modulated and frequency-modulated (AM-FM) radar with an active reflector to produce high-accuracy distance measurements is proposed and demonstrated in this paper. The proposed radar consists of an AM-FM base module and an active reflector. The combination of AM and FM modulations resolves ambiguity of the absolute distance in typical AM radars, while improving range accuracy in typical FM radars with narrow bandwidth. Also, the active reflector, which translates the frequency of the received signal, resolves the problem of phase detection interference due to the direct Tx-to-Rx leakage in AM radars. In this paper, the operating principle, experimental tests, and analysis are presented. The implemented AM-FM radar operates in X-band (Tx: 10.5 GHz, Rx: 8.5 GHz) with the 620 MHz bandwidth. The measured range accuracy of less than ±10 mm at a distance of 70 m is obtained