Diverse effect of BMP-2 homodimer on mesenchymal progenitors of different origin

Bone morphogenetic protein-2 (BMP-2), is a potential factor to enhance osseointegration of dental implants. However, the appropriate cellular system to investigate the osteogenic effect of BMP-2 in vitro in a standardized manner still needs to be defined. The aim of this study was to examine the effect of BMP-2 on the cell proliferation and osteogenic differentiation of human osteogenic progenitors of various origins: dental pulp stem cells (DPSC), human osteosarcoma cell line (Saos-2) and human embryonic palatal mesenchymal cell line (HEPM). For induction of osteogenic differentiation, cell culture medium was supplemented with BMP-2 homodimer alone or in combination with conventionally used differentiation inducing agents. Differentiation was monitored for 6-18 days. To assess differentiation, proliferation rate, alkaline phosphatase activity, calcium deposition and the expression level of osteogenic differentiation marker genes (Runx2, BMP-2) were measured. BMP-2 inhibited cell proliferation in a concentration and time-dependent manner. In a concentration which caused maximal cell proliferation, BMP-2 did not induce osteogenic differentiation in any of the tested systems. However, it had a synergistic effect with the osteoinductive medium in both DPSC and Saos-2, but not in HEPM cells. We also found that the differentiation process was faster in Saos-2 than in DPSCs. Osteogenic differentiation could not be induced in the osteoblast progenitor HEPM cells. Our data suggest that in a concentration that inhibits proliferation the differentiation inducing effect of BMP-2 is evident only in the presence of permissive osteoinductive components. beta-glycerophosphate, was identified interacting with BMP-2 in a synergistic manner

Nomenclature, taxonomy and distribution of Crataegus lindmanii

The nomenclature of Crataegus lindmanii Hrab.-Uhr. is overviewed and clarified. A new combination (Crataegus lindmanii Hrab.-Uhr. var. ronnigeri (K. Malý) Kerényi-Nagy) is done and a new variety (Crataegus lindmanii var. jodalii) is described with new localities of in Hungary (Visegrád, Mt Ágas) and in Serbia (Fruška Gora, Krušedol near Vrdnik / Фрушка Гора, Крушедол, Врдник; Stara Planina Mts, Basara-Vidlič / Стара планина, Ьасара-Видлич). Determination key for infraspecific taxa, comparison of the taxa, a draft and distribution map of the species in Europe and in the Carpathian Basin are also given

Rosa × braunii, a new rose hybrid in Slovakia

A new rose hybrid was found in Slovakia, Malé Karpaty Mts, close to the Plavecky Peter on the hill Ježovka. This rose taxon called Rosa × braunii was described by Jenő B. Keller from the Bruck an der Leitha (Austro-Hungarian Empire) in 1882. It is a hybrid of R. tomentosa Sm. and R. spinosissima L. Its morphology, diagnostic features, chorology and conservational status are discussed. Legal protection of this taxon is highly recommended, because this is its second population in the world, the only currently known locality, and it is a unique taxon, not an introgressive hybrid

Three new rose microspecies from sect. Rubiginosae in Slovakia

The article deals with the distribution of three species from the genus Rosa in Slovakia. Field research, sampling and herbarium sheet revision at the Herbarium of Department of Botany, Faculty of Natural Sciences Comenius University (SLO) and the Herbarium of Institute of Botany, Slovak Academy of Sciences (SAV) showed new data on the distribution of Rosa pocsii Kerényi-Nagy, Rosa polyacantha (Borbás) H. Br. and Rosa zagrabiensis Vuk. et H. Br. in Slovakia. Detailed taxonomic description, distribution of selected taxa in Slovakia, a determination key and a comparative table of the sect. Rubiginosae are given

Clinical Investigation of Hereditary and Acquired Thrombophilic Factors in Patients with Venous and Arterial Thromboembolism

Erzsebet Kovács,1 Zsuzsanna Bereczky,2 Adrienne Kerényi,3 Renáta Laczik,4 Valéria Nagy,5 Dávid Ágoston Kovács,6 Sándor Kovács,7 György Pfliegler1 1Centre of Rare Diseases, Department of Internal Medicine, University of Debrecen, Debrecen, Hungary; 2Division of Clinical Laboratory Science, Department of Laboratory Medicine, University of Debrecen, Debrecen, Hungary; 3Department of Laboratory Medicine, University of Debrecen, Debrecen, Hungary; 4Division of Angiology, Department of Internal Medicine, University of Debrecen, Debrecen, Hungary; 5Department of Ophthalmology, University of Debrecen, Debrecen, Hungary; 6Department of Surgery, University of Debrecen, Debrecen, Hungary; 7Department of Research Methodology and Statistics, Institute of Sectoral Economics and Methodology, University of Debrecen, Debrecen, HungaryCorrespondence: Erzsebet Kovács, Centre of Rare Diseases, Department of Internal Medicine, Faculty of Medicine, University of Debrecen, 98 Nagyerdei krt, Debrecen, H-4032, Hungary, Tel +36 52 255574, Email [email protected]: The clinical relevance of thrombophilic laboratory factors, especially the “mild” ones, and the need for their screening is not generally recommended in venous (VTE) and/or arterial (ATE) thromboembolism.Methods: Our aim was to investigate possible associations between comorbidities and 16 inherited/acquired “severe” and “mild” laboratory thrombophilic factors (detailed in introduction) in patients (n=348) with VTE/ATE without a serious trigger (high-risk surgical intervention, active cancer and/or chemo-radiotherapy). Cases with VTE/ATE were enrolled when the thrombotic event occurred under the age of 40, in case of positive family history, recurrent thromboembolism, idiopathic event or unusual location. Patients without a detailed thrombophilia screening or who suffered from both ATE/VTE were excluded to find potential distinct thrombosis type specific thrombophilic risks. The possible role of “mild” factor accumulation was also investigated in VTE (n=266).Results: Elevation of factor VIII clotting activity was associated with VTE rather than ATE. Varicose veins together with postthrombotic syndrome were strongly related to several “mild” factors. Besides “severe” we found that the “mild” thrombophilic factors were also strongly associated with VTE/ATE. Comorbidities/conditions such as diabetes and smoking were generally associated with hyperlipidemia; moreover, both had a correlation with lipoprotein (a) in VTE. We also revealed an important contribution of “mild” factors in increasing trends of several types and localizations of VTE.Conclusion: In summary, besides the “severe” thrombophilic factors, the “mild” ones also seem to play a non-negligible role in the manifestation of thrombosis, especially in combination. Therefore, an extended screening might be useful in the personalized recommendation of antithrombotic prophylaxis.Keywords: thrombosis, hemostasis, thrombophilia, venous thromboembolism, arterial thromboembolis