7 research outputs found
Lentinan Degradation in the <i>Lentinula edodes</i> Fruiting Body during Postharvest Preservation Is Reduced by Downregulation of the <i>exo</i>-β-1,3-Glucanase EXG2
Lentinan from <i>Lentinula edodes</i> fruiting bodies
(shiitake mushrooms) is a valuable β-glucan for medical purposes
based on its anticancer activity and immunomodulating activity. However,
lentinan content in fruiting bodies decreases after harvesting and
storage due to an increase in glucanase activity. In this study, we
downregulated the expression of an <i>exo</i>-β-1,3-glucanase, <i>exg2</i>, in <i>L. edodes</i> using RNA interference.
In the wild-type strain, β-1,3-glucanase activity in fruiting
bodies remarkably increased after harvesting, and 41.7% of the lentinan
content was lost after 4 days of preservation. The EXG2 downregulated
strain showed significantly lower lentinan degrading activity (60–70%
of the wild-type strain) in the fruiting bodies 2–4 days after
harvesting. The lentinan content of fresh fruiting bodies was similar
in the wild-type and EXG2 downregulated strains, but in the downregulated
strain, only 25.4% of the lentinan was lost after 4 days, indicating
that downregulation of EXG2 enables keeping the lentinan content high
longer
Grouping of multicopper oxidases in Lentinula edodes by sequence similarities and expression patterns
The edible white rot fungus Lentinula edodes possesses a variety of lignin degrading enzymes such as manganese peroxidases and laccases. Laccases belong to the multicopper oxidases, which have a wide range of catalytic activities including polyphenol degradation and synthesis, lignin degradation, and melanin formation. The exact number of laccases in L. edodes is unknown, as are their complete properties and biological functions. We analyzed the draft genome sequence of L. edodes D703PP-9 and identified 13 multicopper oxidase-encoding genes; 11 laccases in sensu stricto, of which three are new, and two ferroxidases. lcc8, a laccase previously reported in L. edodes, was not identified in D703PP-9 genome. Phylogenetic analysis showed that the 13 multicopper oxidases can be classified into laccase sensu stricto subfamily 1, laccase sensu stricto subfamily 2 and ferroxidases. From sequence similarities and expression patterns, laccase sensu stricto subfamily 1 can be divided into two subgroups. Laccase sensu stricto subfamily 1 group A members are mainly secreted from mycelia, while laccase sensu stricto subfamily 1 group B members are expressed mainly in fruiting bodies during growth or after harvesting but are lowly expressed in mycelia. Laccase sensu stricto subfamily 2 members are mainly expressed in mycelia, and two ferroxidases are mainly expressed in the fruiting body during growth or after harvesting, and are expressed at very low levels in mycelium. Our data suggests that L. edodes laccases in same group share expression patterns and would have common biological functions
MOESM1 of Grouping of multicopper oxidases in Lentinula edodes by sequence similarities and expression patterns
Additional file 1. Additional information of L. edodes multicopper oxidases, such as signal peptide prediction, enzyme purificaition, expression patterns, primer design, data set for phylogenetic analysis, and sequence infromation