Mechanism of Genomic Instability in Cells Infected with the High-Risk Human Papillomaviruses

In HPV–related cancers, the “high-risk” human papillomaviruses (HPVs) are frequently found integrated into the cellular genome. The integrated subgenomic HPV fragments express viral oncoproteins and carry an origin of DNA replication that is capable of initiating bidirectional DNA re-replication in the presence of HPV replication proteins E1 and E2, which ultimately leads to rearrangements within the locus of the integrated viral DNA. The current study indicates that the E1- and E2-dependent DNA replication from the integrated HPV origin follows the “onion skin”–type replication mode and generates a heterogeneous population of replication intermediates. These include linear, branched, open circular, and supercoiled plasmids, as identified by two-dimensional neutral-neutral gel-electrophoresis. We used immunofluorescence analysis to show that the DNA repair/recombination centers are assembled at the sites of the integrated HPV replication. These centers recruit viral and cellular replication proteins, the MRE complex, Ku70/80, ATM, Chk2, and, to some extent, ATRIP and Chk1 (S317). In addition, the synthesis of histone γH2AX, which is a hallmark of DNA double strand breaks, is induced, and Chk2 is activated by phosphorylation in the HPV–replicating cells. These changes suggest that the integrated HPV replication intermediates are processed by the activated cellular DNA repair/recombination machinery, which results in cross-chromosomal translocations as detected by metaphase FISH. We also confirmed that the replicating HPV episomes that expressed the physiological levels of viral replication proteins could induce genomic instability in the cells with integrated HPV. We conclude that the HPV replication origin within the host chromosome is one of the key factors that triggers the development of HPV–associated cancers. It could be used as a starting point for the “onion skin”–type of DNA replication whenever the HPV plasmid exists in the same cell, which endangers the host genomic integrity during the initial integration and after the de novo infection

Eesti osalemine 6. raamprogrammis

Euroopa Liidu teadus- ja arendustegevuse 6. raamprogramm (2002-2006).Väikeriigi teadlastele on aktiivne rahvusvaheline koostöö üheks ellujäämise tagatiseks. Eesti teadlased on seda teadnud läbi aegade ning kinni haaranud kõigist pakutavatest võimalustest. Neist üheks on olnud EL teaduse ja arendustegevuse alased raamprogrammid, milles osalemist alustati kohe, kui raudne eesriie vähegi paotus, ehkki ametlikult sai Eesti osalemisõiguse alles 5. raamprogrammis 1999. aastal. Seega võime praegusel hetkel öelda, et meil on selja taga juba üle kümne aasta raamprogrammides osalemise kogemust. Käesolev trükis annab teile ülevaate Eesti osalusest 6. raamprogrammis (6RP). Kuuenda raamprogrammi (2002–2006) põhieesmärgiks oli kaasa aidata Euroopa killustunud teadusmaastiku kujunemisele ühtseks Euroopa teadusruumiks

Papilloomiviiruse DNA replikatsioon põhjustab peremeesrakus geneetilist ebastabiilsust

Genetic information in metazoan cells is stored in condensed chromatin, which protects the DNA from damage. However, when this information needs to be accessed, the secured DNA is decondensed as the double helix itself is opened. It is during DNA replication that DNA is the most vulnerable and therefore genomic duplication is highly orchestrated; it is carefully planned during the G1 phase, precisely executed in the S-phase, and all the mistakes are eliminated in the S- and G2 phases before mitosis. The whole process is closely guarded by cellular control factors that intervene immediately at the slightest deviation from the plan. Papillomaviruses are endogenous parasites that do not require the same kind of fidelity as metazoan cells and can afford some inaccuracy while replicating their small circular double stranded DNA (dsDNA) genome. They have evolved systems either to overrun the cellular defenses and to replicate their genome mul­­tiple times per cell cycle or to mimic cellular DNA replication by re­plicating approximately once per cell cycle. In either case, the genomic integrity of the host cell is not jeopardized during the normal viral life cycle. However, the human papillomavirus (HPV) is occasionally integrated into the host genome. The mechanism of viral integration is currently unknown, but the current work demonstrated that the inability to precisely replicate its DNA once per cell cycle makes the HPV act like a Trojan horse while inside the host genome. If not correctly regulated, it will not only end the viral life cycle, but it can also cause the death of the host by causing chromosomal instability, which may lead to cancer. Normaalselt kulgeva viiruse elutsükli korral püsib inimese papilloomiviiruse (HPV) genoom latentselt nakatunud rakkudes episomaalse multikoopialise plasmii­dina. Samas on aga avastatud, et enamuses emakakaelavähi rakkudes on osa viiruse DNAst, mis sisaldab viiruse replikatsiooni alguspunkti ning E6 ja E7 avatud lugemisraame, integreerunud peremeesraku genoomi. Lisaks on tões­tatud, et HPV integratsiooni käigus läbitakse etapp, kus plasmiidne ja integree­runud HPV DNA eksisteerivad ühtedes rakkudes. Seega on võimalik, et vähi­tekkeni viinud geneetilised ümberkorraldused on vähemalt osaliselt saanud alguse integreeritud HPV DNA re-replikatsioonist, mis algatati plasmiidselt HPV-lt ekspresseeritud viiruse replikatsioonivalkude vahendusel. Käesolevas töös näidati esmalt veise papilloomiviiruse tüüp 1 (BPV1) ja p53 valgu näitel, et papilloomiviirustel on potentsiaali varjata oma genoomi re-replikatsiooni toimumist rakuliste kontrollmehhanismide eest. Järgnevalt demonst­reeriti, et HPV valgud E1 ja E2 on võimelised algatama DNA replikat­siooni ka integreeritud HPV-lt ning, et see viib madalmolekulaarsete DNA molekulide, muulhulgas ekstrakromosomaalsete plasmiidide, tekkeni. Lisaks selgus, et integreerunud HPV-lt alguse saanud DNA replikatsioon ulatub ka ümbritsevate rakuliste järjestusteni ning, et see viib teatud ümberkorraldusteni raku genoomis. Antud töös tuvastati nii HPV integratsioonilookuse duplikat­sioon kui ka kromosoomide vaheline translokatsioon. Immuno­fluorestsents­analüüsi ja immunosadestamise tulemused näitasid, et integreeritud HPV repli­kat­siooni poolt tekitatud DNA katkete likvideerimiseks aktiveeritakse rakulised DNA reparatsiooni faktorid. Kokkuvõttes võib järeldada, et integreeritud papilloomiviiruse DNA replikat­­sioon ning sellele vastuseks peremeesraku poolt käivitatud DNA repa­ratsioon võivad viia geneetilise ebastabiilsuseni ja vähkkasvaja tekkeni

Replication centers of the integrated HPV recruit Mre11-Nbs1-Rad50 complex and Ku70/80 heterodimer.

<p>HeLa cells were transfected and analyzed as described previously. Co-immunostaining of HPV18 E1 (Alexa Fluor 568, second column) and the following proteins are presented: Mre11, Nbs1, Rad50, and Ku70/80 proteins (Alexa Fluor 488, first column). The localizations of the E1 and the respective DNA repair proteins are also shown in the third column as a merged image and DAPI stained nuclei are presented in the fourth column.</p

Repair of the amplified HPV18 integration locus is coordinated by the ATM and ATR signaling pathways.

<p>HeLa cells were transfected and analyzed as described previously. Co-immunostaining of HPV18 E1 (Alexa Fluor 568, second column) and the following proteins are shown at the figure: ATM, Chk2, ATRIP, and Chk1 (S317) (Alexa Fluor 488, first column). Merged images are shown in the third column and DAPI stained nuclei in the fourth column.</p

Mechanism of the genomic instability of the cells harboring the replication origin of integrated HPV.

<p>If HPV plasmid is present in the cells harboring integrated HPV, the DNA re-replication from the integrated HPV origin is initiated, and ATM and ATR signaling pathways respond, respectively, to the produced DSBs and ssDNA. In most cases, the cells will either become apoptotic or the damage sites will be properly repaired by homologous recombination and non-homologous end-joining. In addition, duplications within the HPV locus have previously been detected. In the current study, the excision and generation of extrachromosomal copies of the HPV locus and cross-chromosomal translocations were also detected. All these mutations require DSBs that could be generated, such as through head-to-tail fork collision or by encounter of the re-replication fork with the Okazaki fragments of the previous fork. Supported by our observations, we speculate that the cellular defense against the integrated HPV re-replication is primary coordinated by ATM. ATR pathways do not interfere with a properly working E1-driven replication fork but, rather, are responsible for the repair of the damage that is caused by fork collision and dissociation that reveals the RPA coated ssDNA without the generation of DSBs. DNA replication/repair factors that are shown to be within the HPV replication centers in the current work, are colored in the figure.</p

DNA replication initiated from the integrated HPV origin generates various low-molecular-weight DNA products.

<p>High-molecular-weight (HMW) DNA and low-molecular-weight (LMW) DNA were fractionated and purified by Hirt lysis 24 hrs post-transfection from HeLa cells (A) and from SiHa cells (B) that were both transfected as follows: mock-transfection (lanes 1 and 5); 5 µg of HPV18 E1 expression plasmid (lanes 2 and 6); 2 µg of E2 expression plasmid (lanes 3 and 7); 5 µg of HPV18 E1 and 2 µg of E2 expression plasmids (lanes 4 and 8). A 3 µg portion of HMW DNA and three times the respective amount of LMW DNA were digested with HindIII (A) or Acc65I/BshTI (B) and separated on a one-dimensional gel. The integrated HPV URR-specific signals were detected by Southern blot analysis. (C) Schematic presentation of the migration of dsDNA linear, supercoiled, and open circle molecules on 2D neutral-neutral gels. (D, E) 5 µg of HPV18 E1 and 2 µg of E2 expression plasmids were transfected into HeLa cells and LMW DNA was purified by Hirt lysis 48 hrs post-transfection. Extracted DNA was digested with DpnI and fractionated by the CsCl-ethidium bromide density gradient. The fraction of linear fragments (lin) and open circular molecules (oc) (D) and the fraction of supercoiled circular plasmids (sc) (E) were separated on a 2D gel, transferred to a nylon filter, and probed with the HPV18 genomic fragment (from nt 3917 to1575). Numbers shown on the axes represent the markers of linear (lin) and supercoiled circular (sc) DNA forms. Black arrowhead indicates the shift that was caused by mtDNA. (F) Schematic presentation of HPV16 integration locus within chromosome 13 in SiHa cells, where the cleavage sites of Acc65I, Eco91I, BshTI, and BcuI as well as their distances from HPV origin are presented. (G) Schematic presentation of the migration of replication forks and replication puffs on 2D neutral-neutral gels. (H,I) SiHa cells were co-transfected with 5 µg of HPV18 E1 and 2 µg of E2 expression plasmids. LMW DNA was extracted 24 hrs post-transfection and digested with Acc65I-BshTI (H) and Eco91I-BcuI (I). Respective HPV16 genome fragments were used as probes on 2D Southern blots. (J) SiHa cells were co-transfected with 1 µg of HPV18 E1 and 2 µg of E2 expression plasmids. Extracted LMW DNA was digested with Eco91I-BcuI and analyzed by 2D Southern blots.</p

Activation of the ATM-Chk2 signaling pathway.

<p>(A–C) HeLa cells were transfected as follows: 5 µg of HPV18 E1 and 2 µg of HPV18 E2 expression plasmids (lane 1); 5 µg of HPV18 E1 expression plasmid (lane 2); 2 µg of E2 expression plasmid (lane 3); and a mock-transfection (lane 4). In every transfection, the amount of plasmid was adjusted to 10 µg with a carrier plasmid (pauxoMCF). Non-transfected HeLa cells are presented in lane 5 and HeLa cells that were treated 1 h with etoposide (50 µM) prior to the analysis in lane 6. Western blot analyses were performed at a 24 hrs time point to detect HPV18 E1 (A, panel a), HPV18 E2 (A, panel b), gamma histone H2AX (phosphorylated at S139) (A, panel c), and β-actin (A, panel d). Western blot analyses of Chk2 phosphorylated at Thr68 and Ser19 were performed after the immunoprecipitation with the anti-Chk2 antibody (B, panels a, b, c). Chk1 phosphorylated at Ser317 was detected from extracts that were immunoprecipitated with the anti-Chk1 antibody (C, panels a, b). (D) HeLa cells were transfected either with 2 µg of circular HPV18 genome, 2 µg of pBabePuro and 6 µg of carrier plasmid (lane 1), or with 2 µg of pBabePuro and 8 µg of carrier plasmid (lane 2). Untransfected cells were removed with puromycin treatment (2 µg/ml) 24–48 h posttransfection. Western blot analyses with anti-Chk2 (panel a) and anti-Chk2-Ser19 (panel b) antibodies were performed after the immunoprecipitation with the anti-Chk2 antibody at a 72 h time point. Untreated HeLa cells are shown in lane 3 and etoposide-treated HeLa cells in lane 4.</p

The HPV genome induces genomic instability in cells harboring the integrated HPV.

<p>Southern blot analyses of the HeLa (A, B) and SiHa cells (C, D) co-transfected with 1 µg of HPV16 genome and 1 µg of linearized pEGFPN-1 (A, C) or with 1 µg of HPV18 genome and 1 µg of linearized pEGFPN-1 (B, D). Low-molecular-weight DNA was extracted 24 h and 48 h after transfection and digested with restriction enzymes, as indicated in the figure. HPV plasmids were detected with a ³²P-labeled HPV16 or HPV18 genome probe, respectively. (E, F) The restriction analyses of untreated SiHa cells (E, lanes 1–3), HPV16-transfected SiHa cells five weeks post-transfection (E, lanes 4–6), HPV18-transfected SiHa cells five weeks post-transfection (E, lanes 13–15; F, lanes 1–3), as well as the subclones of the HPV16-transfected SiHa cells (E, lanes 7–12) and HPV18-transfected SiHa cells (E, lanes 16–18; F, lanes 4–6). Digested total DNA was analyzed by Southern blotting, where the HPV16 (E) or HPV18 (F) genomes were used as probes. Restriction enzymes are indicated in the figure.</p

DNA replication of the integrated HPV takes place at specific nuclear foci.

<p>HeLa and SiHa cells were co-transfected with 5 µg of HPV18 E1 and 2 µg of E2 expression plasmids. Cells were analyzed 20 hrs post-transfection. (A) Co-immunostaining of E1 (Alexa Fluor 568, first column) and BrdU (FITC, second column). Cells were pulse-labeled with BrdU for 2 hrs prior to IF analysis. In the third column, the localizations of E1 and BrdU in the same cells are presented as a merged image. (B) Combined immunofluorescence and FISH analysis to detect the integrated HPV DNA (Alexa Fluor 488, first column) and the HPV E1 protein (Alexa Fluor 568, second column) in SiHa and HeLa cells. Localizations of the E1 and the integrated HPV DNA are also presented in the third column as a merged image. DNA was counterstained with DAPI (fourth column).</p