Mir-129-2-3p Has Tumor Suppressor Role in Ewing Sarcoma Cell Lines and Cancer Tissue Samples

Deregulation of miRNA expressions was identified as a novel feature of tumor biology in Ewing sarcoma (EWS). The aim was to evaluate the regulatory role of miR-129-2-3p in EWS cell lines and human EWS tissue samples. EWS cell lines TC-71, TC-106, and CHLA-99 were used in the study and real-time PCR was utilized to investigate the functional role of tumor suppressor mir-129-2-3p and miR-129-2-3p levels in the cells. Proliferation, migration, invasion and apoptosis assays were carried out within the scope of functional in vitro studies. Expression levels of CDK6 and SOX4, which are miR-129-2-3p target genes, were examined. Moreover, the change in expression levels of miR-129-2-3p in EWS tumor tissues was also examined. It was determined that miR-129-2-3p expression markedly diminished in all the studied cell lines. In addition, miR-129-3p was found to decrease in proliferation, migration, invasion and apoptosis assays in all EWS cell lines. CDK6 and SOX4 levels were also decreased in miR-129-2-3p transfected cell lines. It was found that miR-129-2-3p levels were significantly decreased in EWS tumor tissue samples compared to the corresponding adjacent normal tissue samples. In line with the results of our current study, where the possible function of miR-129-2-3p in EWS cell lines was examined, for the first time in the literature miR-129-2-3p was shown to have low expression level in EWS lines and EWS tumor tissue samples, and to provide a tumor suppressor effect

Giant osteoid osteoma of the ethmoid bone with unusual large nidus

Osteoid osteoma (OO) is a benign bone neoplasm which is seen in the long bones of appendicular skeleton. It is rarely seen in the cranium.Ethmoid bone OO has been very rarely reported so far. We report another case of giant osteoid osteoma involving the ethmoid bone with intraorbital and intracranial extension in a 3O year old female patient. This case with unusual anatomical location was presented. It is first time reporting a giant osteoid osteoma of ethmoid bone with such a large nidus

The effects of locally applied procaine on wound healing

Objective: The goals of this study were: (1) To determine the efficacy of 2% procaine (the most commonly used concentra- tion) in wound healing; and (2) To determine the proper open wound injection site. Materials and Methods: Thirty adult male Sprague-Dawley rats weighing between 250 and 350 g were used. Two full thickness defects were made on two sides of the midline 1 cm away from midline. The skin wound areas were approxi- mately 1.5 cm and times; 1.5 cm. The animals were randomly divided into three groups: Group 1 (control group, n = 8), Group 2 (injection directly into the base of wound, n = 8), and Group 3 (injection into healthy skin around the peripheral margins of the wound, n = 8). Mechanical analyses of wound tensile strength of were evaluated in all groups. Results: Wound closure was first seen in Group 3 on day 14. Mean wound healing times were 18.25 days, 16.25 days, and 15.62 days, and mean tensile strength was 777.13 cN, 988.25 cN, and 1068.25 cN in the Groups 1, 2, and 3 respectively. Conclusions: Procaine did not cause any necrosis around the wound, did not retard wound healing, did not cause circu- lation deficiency, and did not reduce the breaking strength of the wound. Therefore, it can be safely used to reduce pain around the wound and to accelerate the healing process of slow-to-heal wounds. [Arch Clin Exp Surg 2015; 4(1.000): 41-45

Anterior orbital leiomyoma originating from the supraorbital neurovascular bundle

Purpose: To present a young female patient with left anterior orbital leiomyoma that originates from the supraorbital neurovascular bundle. Case presentation: A 41-year-old female patient was admitted to our clinic with a complaint of swelling of the left upper eyelid. Based on the ophthalmological and imaging assessments, the excisional biopsy with the preliminary diagnosis of dermoid cyst was planned. The histopathological and immunohistochemical examinations of excised sample revealed surprisingly that the tumour was a leiomyoma. No recurrence was detected in the patient’s follow-up. Conclusion: Although it is rare, orbital leiomyoma should be considered in the differential diagnosis of patients with orbital tumour

Primary Lymphoma of the Ovary: A Case Report

WOS: 000360662600027Ovarian lymphoma is a rare malignancy. It can occur in two ways, primary or secondary. The tumor may act like it is an epithelial carcinoma. A case with a diagnosis of diffuse large B cell lymphoma as a primary tumour of the ovary was presented with the histopathologic and immunohistochemical profile and clinical features in order to enrich the awareness to the early diagnosis and treatment

Polypoid Lesions of Gallbladder: Retrospective Analysis of 99 Cases.

Purpose: Polypoid lesions of gallbladder are originated from gallbladder mucosa. In the current study, it has been aimed to evaluate clinically and histopathologically the polypoid lesions of cholecystectomy cases in ten years. Materials and Methods: In our clinic 4479 cholecystectomy cases between June 2004 and June 2014 have been retrospectively investigated for the polypoid lesions clinically and histopathologically. Results: In 4479 cholecystectomy cases, 99 (2.2%) included polypoid lesions. Abdominal pain has been the major symptom. In the histopathologic evaluation, 42 cholesterol polyps, 2 inflamatuar polyps, 2 hyper plastic polyps, 2 intestinal type adenoma, 18 adenomyoma, 29 adenomyomatous hyperplasia 1 pyloric gland adenoma, 1 musinous cyst adenoma, 1 tubular adenoma, 1 well-differentiated papillary adenocarcinoma have been determined. Conclusion: A retrospective analysis has been performed for the polypoid lesions of the cholecystectomy clinically and histopathologically. [Cukurova Med J 2015; 40(3.000): 452-456

Renal Involvement in AA Amyloidosis: Clinical Outcomes and Survival

Background: The natural history of AA amyloidosis is typically progressive, leading to multiple organ failure and death. We analyzed the etiology as well as clinical and laboratory features of patients with biopsy-proven AA amyloidosis and evaluated the ultimate outcome. Methods: Seventy-three patients (24 female; mean age 41.85±15.89 years) were analyzed retrospectively. Demographic, clinical and laboratory features were studied and the outcome was assessed. Results: Familial Mediterranean Fever and tuberculosis were the most frequent causes of amyloidosis. Mean serum creatinine and proteinuria at diagnosis were 4.65±4.89 mg/dl and 8.04±6.09 g/day, respectively; and stage I, II, III, IV and V renal disease were present in 19.2%, 13.7%, 16.4%, 11%, and 39.7% of the patients, respectively. ESRD developed in 16 patients during the follow-up period. All of the ESRD patients started a dialysis programme. Thirty patients (41%) died during the follow-up period; median patient survival was 35.9±6.12 months. Old age, tuberculosis etiology, advanced renal disease and low serum albumin levels were associated with a worse prognosis. Serum albumin was a predictor of mortality in logistic regression analysis. Conclusion: The ultimate outcome of the patients with AA amyloidosis is poor, possibly due to the late referral to the nephrology clinics. Early referral may be helpful to improve prognosis

Solid-pseudopapillary neoplasm of the pancreas: Clinicopathologic and immunohistochemical analysis of nine cases

Background: Solid-pseudopapillary neoplasm (SPPN) of the pancreas is a distinctive tumor of low malignant potential with a predilection for female patients in the second and third decades of life. We studied nine cases of SPPN of the pancreas and reviewed the literature concerning these uncommon tumors. Materials and Methods: A total of 7 cases of SPPN located in the tail of the pancreas and two located in the head of the pancreas were presented. Distal pancreatectomy in three patients and distal pancreatectomy with splenectomy in two patients Whipple′s operation in four patients were performed. Histological diagnosis was made by performing hematoxylin-eosin and periodic acid-Schiff staining, immunohistochemical staining. Follow-up of the patients was between 2 months and 12 years. Results: Computed tomography and magnetic resonance imaging were found as equivocal for diagnosis. Mass containing cystic and solid areas were not characteristic but raised suspicion of SPPN. Pathologic examination showed SPPN in all patients. No metastasis or recurrence was detected during follow-up. Conclusions: Solid-pseudopapillary neoplasm is a relatively rare tumor, and patients tend to survive for a long period. Preoperative imaging is not characteristic. Pathologic examination is the mainstay in the diagnosis. Complete surgical removal is the best choice of treatment