In vitro exposure to di-(2-ethylhexyl) phthalate (DEHP) stimulates spontaneous feline uterine contractions

Yildirim, Ebru/0000-0002-6289-0729; Kabakci, Ruhi/0000-0001-9131-0933; Elifoglu, Taha Burak/0000-0002-2302-6321WOS:000560988800020Di-(2-ethylhexyl) phthalate (DEHP) is widely used as a plasticizer in various products such as PVC-derived plastics, toys, packaging materials, cosmetics, and pharmaceuticals. This study aimed to investigate the effect of DEHP on spontaneous contractions of the feline uterus in vitro. Tubal 1-cm uterine samples prepared from 10, 9, and 12 uteri obtained from adult cats in estrus (n = 5), diestrus (n = 5), and interestrus (n = 5), respectively, after ovariohysterectomy were suspended in an isolated organ bath in aerated Krebs solution at 39 +/- 1 degrees C, and an initial 1 g tension was given. After 1 h equilibration of tissues, the spontaneous contractions were recorded for 10 min as control. The effects of solvent and DEHP (0.001-100 mu M) on contractions were then evaluated in terms of frequency and mean amplitude parameters. It was observed that DEHP had no effect on uterine contractions of cats in interestrus. However, DEHP significantly increased the mean amplitude of uterine contractions during the estrus and diestrus periods at concentrations of 1 mu M and 10 mu M, respectively, depending on the dose (P 0.05). 'this study, carried out for the first time in cats, showed that DEHP has a stimulatory effect on uterine contractions. We concluded that disruption of the uterine contractions, which are essential for physiological reproductive processes such as regular estrous cycles, sperm and zygote transport, implantation and continuation of pregnancy, by DEHP exposure may cause many reproductive problems

Effects of bisphenol A, diethylhexyl phthalate and pentabrominated diphenyl ether 99 on steroid synthesis in cultured bovine luteal cells

KABAKCI, Ruhi/0000-0001-9131-0933; YIgit, Ayse Arzu/0000-0001-5837-6877WOS:000520762900001PubMed: 32125030Bisphenol A (BPA), diethylhexyl phthalate (DEHP) and pentabrominated diphenyl ether 99 (PBDE 99) are environmental toxicants belonging to the endocrine disrupting compounds (EDCs). They exert adverse effects on the various physiological systems, especially the reproductive system of humans and animals. The aim of this study was to investigate the effects of BPA, DEHP and PBDE 99 on progesterone (P4) synthesis in cultured bovine luteal cells. The bovine luteal cells isolated from the mid-luteal corpora lutea were exposed to different concentrations of BPA (1, 3, 10 and 30 mu M), DEHP (1, 3, 10 and 30 mu M) and PBDE 99 (0.1, 0.3, 1 and 3 mu M) in a serum-free culture media for 48 and 96 hr. At 48 hr, the P4 level in the luteal cells decreased after treatment with all concentrations of BPA; 3, 10 and 30 mu M of DEHP; and 3 mu M of PBDE 99 compared to the control (p < .05). Treatment of cells with 3-30 mu M of BPA, 1-30 mu M of DEHP and 1-3 mu M of PBDE 99 for 96 hr resulted in reduction in P4 synthesis (p < .05). However, lower concentrations of PBDE 99 (0.1 and 0.3 mu M) increased P4 levels at 48 and 96 hr. Synthesis of P4 was lower at 96 hr compared to the 48 hr in the groups treated with BPA (30 mu M), DEHP (1-30 mu M), PBDE 99 (0.3-3 mu M) and control group. Our results showed that BPA, DEHP and PBDE 99 are able to alter luteal steroidogenesis in bovine cells and can disrupt hormonal balance in the ovary. However, it is necessary to evaluate the exact mechanism underlying these effects in future studies.Kirikkale University, Scientific Research Projects Coordination UnitKirikkale University [2015/129]Kirikkale University, Scientific Research Projects Coordination Unit, Grant/Award Number: 2015/12

The Influence of Pentabrominated Diphenylether 99 on Progesterone Secretion by Bovine Luteal Cells

KABAKCI, Ruhi/0000-0001-9131-0933WOS: 000419806200211…KU [SRPCU: 2015/129]Supported by the KU SRPCU: 2015/129 and this report is a part of the PhD thesis of R KABAKC

Effects of bisphenol A, diethylhexyl phthalate and pentabrominated diphenyl ether 99 on steroid synthesis in cultured bovine luteal cells

WOS: 000520762900001PubMed: 32125030Bisphenol A (BPA), diethylhexyl phthalate (DEHP) and pentabrominated diphenyl ether 99 (PBDE 99) are environmental toxicants belonging to the endocrine disrupting compounds (EDCs). They exert adverse effects on the various physiological systems, especially the reproductive system of humans and animals. The aim of this study was to investigate the effects of BPA, DEHP and PBDE 99 on progesterone (P4) synthesis in cultured bovine luteal cells. The bovine luteal cells isolated from the mid-luteal corpora lutea were exposed to different concentrations of BPA (1, 3, 10 and 30 mu M), DEHP (1, 3, 10 and 30 mu M) and PBDE 99 (0.1, 0.3, 1 and 3 mu M) in a serum-free culture media for 48 and 96 hr. At 48 hr, the P4 level in the luteal cells decreased after treatment with all concentrations of BPA; 3, 10 and 30 mu M of DEHP; and 3 mu M of PBDE 99 compared to the control (p < .05). Treatment of cells with 3-30 mu M of BPA, 1-30 mu M of DEHP and 1-3 mu M of PBDE 99 for 96 hr resulted in reduction in P4 synthesis (p < .05). However, lower concentrations of PBDE 99 (0.1 and 0.3 mu M) increased P4 levels at 48 and 96 hr. Synthesis of P4 was lower at 96 hr compared to the 48 hr in the groups treated with BPA (30 mu M), DEHP (1-30 mu M), PBDE 99 (0.3-3 mu M) and control group. Our results showed that BPA, DEHP and PBDE 99 are able to alter luteal steroidogenesis in bovine cells and can disrupt hormonal balance in the ovary. However, it is necessary to evaluate the exact mechanism underlying these effects in future studies.Kirikkale University, Scientific Research Projects Coordination UnitKirikkale University [2015/129]Kirikkale University, Scientific Research Projects Coordination Unit, Grant/Award Number: 2015/12

The Effects of Bisphenol A on in Vitro Cat Uterus Contractility

KABAKCI, Ruhi/0000-0001-9131-0933WOS: 000453220100152

Effect of the Incubation Time and High Dose Lead Application on Steroidogenic Luteal Cell Numbers

KABAKCI, Ruhi/0000-0001-9131-0933WOS: 000383578300158

Time- and Dose-dependent Effects of Lead on the Number of Cultured Granulosa Cells Showing Steroidogenic Activity

KABAKCI, Ruhi/0000-0001-9131-0933WOS: 000383578300154

Inhibitory effect of Bisphenol A on in vitro feline uterine contractions

KABAKCI, Ruhi/0000-0001-9131-0933WOS: 000469907300004PubMed: 30967318Bisphenol A (BPA) is an environmental pollutant used as a plasticizer in the manufacture of many plastic products, such as packaging, containers, and water and beverage bottles. There are deleterious effects of BPA on metabolic, endocrine, nervous, and reproductive systems. This is the first study in which there was investigation of the in vitro effect of BPA on the spontaneous contractions of the cat uterus. The tubal uterine segments (1 cm) collected from queens in estrus were suspended in an isolated organ bath. Following tissue stabilization, spontaneous contractions were recorded for 10 min to constitute the control group. The effects of the solvent (alcohol) and BPA at different concentrations (1, 10, and 100 mu M) on uterine contractions were subsequently evaluated at 10 min intervals in terms of frequency and mean amplitude variables. The ethanol vehicle did not alter the uterine contractions compared to the control group. All concentrations of BPA used in the study resulted in a reduction (P < 0.05) in amplitude of uterine contractions in a dose-dependent manner, while only the largest dose of BPA decreased the frequency of contractions (P < 0.05). In reproductive physiology, regular uterine contractions facilitate successful fertilization, migration, implantation, and maintenance of pregnancy, as well as fetus expulsion. The results of this study indicate BPA has an inhibitory effect on spontaneous contractions of the cat uterus. It is proposed that this suppressive effect of BPA on uterine contractions might lead to queen infertility

Selected haematological and biochemical parameters in healthy Damascus goats

KABAKCI, Ruhi/0000-0001-9131-0933WOS: 000364786400279