1,167 research outputs found
Mass transport perspective on an accelerated exclusion process: Analysis of augmented current and unit-velocity phases
In an accelerated exclusion process (AEP), each particle can "hop" to its
adjacent site if empty as well as "kick" the frontmost particle when joining a
cluster of size . With various choices of the
interaction range, , we find that the steady state of AEP can
be found in a homogeneous phase with augmented currents (AC) or a segregated
phase with holes moving at unit velocity (UV). Here we present a detailed study
on the emergence of the novel phases, from two perspectives: the AEP and a mass
transport process (MTP). In the latter picture, the system in the UV phase is
composed of a condensate in coexistence with a fluid, while the transition from
AC to UV can be regarded as condensation. Using Monte Carlo simulations, exact
results for special cases, and analytic methods in a mean field approach
(within the MTP), we focus on steady state currents and cluster sizes.
Excellent agreement between data and theory is found, providing an insightful
picture for understanding this model system.Comment: 13 pages, 8 figure
Transport by molecular motors in the presence of static defects
The transport by molecular motors along cytoskeletal filaments is studied
theoretically in the presence of static defects. The movements of single motors
are described as biased random walks along the filament as well as binding to
and unbinding from the filament. Three basic types of defects are
distinguished, which differ from normal filament sites only in one of the
motors' transition probabilities. Both stepping defects with a reduced
probability for forward steps and unbinding defects with an increased
probability for motor unbinding strongly reduce the velocities and the run
lengths of the motors with increasing defect density. For transport by single
motors, binding defects with a reduced probability for motor binding have a
relatively small effect on the transport properties. For cargo transport by
motors teams, binding defects also change the effective unbinding rate of the
cargo particles and are expected to have a stronger effect.Comment: 20 pages, latex, 7 figures, 1 tabl
Traffic of Molecular Motors
Molecular motors perform active movements along cytoskeletal filaments and
drive the traffic of organelles and other cargo particles in cells. In contrast
to the macroscopic traffic of cars, however, the traffic of molecular motors is
characterized by a finite walking distance (or run length) after which a motor
unbinds from the filament along which it moves. Unbound motors perform Brownian
motion in the surrounding aqueous solution until they rebind to a filament. We
use variants of driven lattice gas models to describe the interplay of their
active movements, the unbound diffusion, and the binding/unbinding dynamics. If
the motor concentration is large, motor-motor interactions become important and
lead to a variety of cooperative traffic phenomena such as traffic jams on the
filaments, boundary-induced phase transitions, and spontaneous symmetry
breaking in systems with two species of motors. If the filament is surrounded
by a large reservoir of motors, the jam length, i.e., the extension of the
traffic jams is of the order of the walking distance. Much longer jams can be
found in confined geometries such as tube-like compartments.Comment: 10 pages, latex, uses Springer styles (included), to appear in the
Proceedings of "Traffic and Granular Flow 2005
Deterministic and stochastic descriptions of gene expression dynamics
A key goal of systems biology is the predictive mathematical description of
gene regulatory circuits. Different approaches are used such as deterministic
and stochastic models, models that describe cell growth and division explicitly
or implicitly etc. Here we consider simple systems of unregulated
(constitutive) gene expression and compare different mathematical descriptions
systematically to obtain insight into the errors that are introduced by various
common approximations such as describing cell growth and division by an
effective protein degradation term. In particular, we show that the population
average of protein content of a cell exhibits a subtle dependence on the
dynamics of growth and division, the specific model for volume growth and the
age structure of the population. Nevertheless, the error made by models with
implicit cell growth and division is quite small. Furthermore, we compare
various models that are partially stochastic to investigate the impact of
different sources of (intrinsic) noise. This comparison indicates that
different sources of noise (protein synthesis, partitioning in cell division)
contribute comparable amounts of noise if protein synthesis is not or only
weakly bursty. If protein synthesis is very bursty, the burstiness is the
dominant noise source, independent of other details of the model. Finally, we
discuss two sources of extrinsic noise: cell-to-cell variations in protein
content due to cells being at different stages in the division cycles, which we
show to be small (for the protein concentration and, surprisingly, also for the
protein copy number per cell) and fluctuations in the growth rate, which can
have a significant impact.Comment: 23 pages, 5 figures; Journal of Statistical physics (2012
MALDI-TOF High Mass Calibration up to 200Â kDa Using Human Recombinant 16 kDa Protein Histidine Phosphatase Aggregates
Background: Protein histidine phosphatase (PHP) is an enzyme which removes phosphate groups from histidine residues. It was described for vertebrates in the year 2002. The recombinant human 16 kDa protein forms multimeric complexes in physiological buffer and in the gas phase. High-mass calibration in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has remained a problem due to the lack of suitable standards. Large proteins can hardly be freed of their substructural microheterogeneity by classical purification procedures so that their use as calibrants is limited. A small adduct-forming protein of validated quality is a valuable alternative for that purpose. Methodology/Principal Findings: Three major PHP clusters of,113, 209 and.600 kDa were observed in gel filtration analysis. Re-chromatography of the monomer peak showed the same cluster distribution. The tendency to associate was detected also in MALDI-TOF MS measuring regular adducts up to 200 kDa. Conclusions/Significance: PHP forms multimers consisting of up to more than 35 protein molecules. In MALDI-TOF MS it generates adduct ions every 16 kDa. The protein can be produced with high quality so that its use as calibration compoun
First detection of Klebsiella variicola producing OXA-181 carbapenemase in fresh vegetable imported from Asia to Switzerland
Background: The emergence and worldwide spread of carbapenemase-producing Enterobacteriaceae is of great concern to public health services. The aim of this study was to investigate the occurrence of carbapenemase-producing Enterobacteriaceae in fresh vegetables and spices imported from Asia to Switzerland.Findings: Twenty-two different fresh vegetable samples were purchased in March 2015 from different retail shops specializing in Asian food. The vegetables included basil leaves, bergamont leaves, coriander, curry leaves, eggplant and okra (marrow). Samples had been imported from Thailand, the Socialist Republic of Vietnam and India. After an initial enrichment-step, carbapenemase-producing Enterobacteriaceae were isolated from two carbapenem-containing selective media (SUPERCARBA II and Brilliance CRE Agar). Isolates were screened by PCR for the presence of bla KPC, bla NDM, bla OXA-48-like and bla VIM. An OXA-181-producing Klebsiella variicola was isolated in a coriander sample with origin Thailand/Vietnam. The bla OXA-181 gene was encoded in a 14′027 bp region flanked by two IS26-like elements on a 51-kb IncX3-type plasmid.Conclusions: The results of this study suggest that the international production and trade of fresh vegetables constitute a possible route for the spread of carbapenemase-producing Enterobacteriaceae. The presence of carbapenemase-producing organisms in the food supply is alarming and an important food safety issue
Sources of stochasticity in constitutive and autoregulated gene expression
Gene expression is inherently noisy as many steps in the read-out of the
genetic information are stochastic. To disentangle the effect of different
sources of stochasticity in such systems, we consider various models that
describe some processes as stochastic and others as deterministic. We review
earlier results for unregulated (constitutive) gene expression and present new
results for a gene controlled by negative autoregulation with cell growth
modeled by linear volume growth.Comment: 7 pages, 6 figures, physica scripta in press (2012
Aberrant Amygdala–Frontal Cortex Connectivity During Perception Of Fearful Faces And At Rest In Generalized Social Anxiety Disorder
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/97272/1/da22014.pd
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