Desorption/Ionization on self-Assembled Monolayer Surface (DIAMS) Evaluation of a New Matrix-Free Laser Desorption/Ionization method

Date du colloque&nbsp;: 06/2008</p

Evaluation of a new matrix-free laser desorption/ionization method through statistic studies: comparison of the DIAMS (desorption/ionization on self-assembled monolayer surface) method with the MALDI and TGFA-LDI techniques

This work demonstrates that the desorption/ionization on self-assembled monolayer surface (DIAMS) mass spectrometry, a recent matrix-free laser desorption/ionization (LDI) method based on an organic target plate, is as statistically repeatable and reproducible as matrix assisted laser desorption ionization (MALDI) and thin gold film-assisted laser desorption/ionization (TGFA-LDI) mass spectrometries. On lipophilic DIAMS of target plates with a mixture of glycerides, repeatability/reproducibility has been estimated at 15 and 30% and the relative detection limit has been evaluated at 0.3 and 3 pmol, with and without NaI respectively. Salicylic acid and its d6-isomer analysis confirm the applicability of the DIAMS method in the detection of compounds of low molecular weight

Desorption/ionization on self-assembled monolayer surfaces (DIAMS): a new matrix-free laser desorption/ionization promising for the analysis of vegetal extracts

Rapid analysis of small molecular weight compounds is one of the most widespread applications of mass spectrometry. The high throughput analyses of vegetal extracts are relatively difficult to perform in MALDI mass spectrometry, since preparation of the sample involves the co-crystallization of the matrix with the analyte. Moreover irradiation of the matrix ion produces many low-m/z vs high-intensity ions preventing the detection of low molecular weight molecules such as secondary metabolites.Our work aims at developing a matrix free alternative to MALDI analysis by the means of an original desorption/ionization on self-assembled monolayers surfaces (DIAMS) technique. We have focused our attention on a monolayer constituted by a 2,2\u27-bithiophene 5-substituted by an alkylthio linked to the gold surface [1]. With the example of salicylate derivatives, we show that the DIAMS method is suitable to the detection and quantification of the low molecular weight compounds. Indeed, the technique is as statistically repeatable and reproducible as other mass spectrometries [2]. This DIAMS method could be suitable to the qualitative and quantitative studies of polar and apolar vegetal extracts without any preliminary chromatographic resolution

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