On the functions of the h subunit of eukaryotic initiation factor 3 in late stages of translation initiation

Reporter transgene assays and comparative polysome-microarray analysis reveal that the intact h subunit of Arabidopsis eIF3 contributes to efficient translation initiation on mRNA leader sequences harbouring multiple uORFs

Rotational Diffusion in a Chain of Particles

We study the coupled rotational diffusion in a two-particle chain on the basis of a Smoluchowski equation and calculate time-correlation functions that are measurable in an experiment. This might be used to explore hydrodynamic interactions in the limit where lubrication theory is valid.Comment: 7 pages, 2 figures, to be published in J. Phys.: Condens. Matte

The trajectory of fidelity in a multiyear trial of the Family Check-Up predicts change in child problem behavior

Therapist fidelity to evidence-based family interventions has consistently been linked to child and family outcomes. However, few studies evaluate the potential ebb and flow of fidelity of therapists over time. We examined therapist drift in fidelity over four years in the context of a Family Check-Up prevention services in early childhood (age 2–5). At age 2, families engaging in Women, Infants, and Children Nutritional Supplement Program (WIC) services were randomized and offered annual Family Check-Ups. Seventy-nine families with a child in the clinical range of problem behaviors at age 2 were included in this analysis. Latent growth modeling revealed a significant linear decline in fidelity over time (M = ?0.35, SD = 0.35) and steeper declines were related to less improvement in caregiver-reported problem behaviors assessed at ages 7.5/8.5 (b = ?.69, p = .003; ? = ?.95, CI: ?2.11 | ?0.22). These findings add to the literature concerning the need to continually monitor therapist fidelity to an evidence-based practice over time to optimize family benefits. Limitations and directions for future research are discussed

Novel components of the Toxoplasma inner membrane complex revealed by BioID.

UNLABELLED:The inner membrane complex (IMC) of Toxoplasma gondii is a peripheral membrane system that is composed of flattened alveolar sacs that underlie the plasma membrane, coupled to a supporting cytoskeletal network. The IMC plays important roles in parasite replication, motility, and host cell invasion. Despite these central roles in the biology of the parasite, the proteins that constitute the IMC are largely unknown. In this study, we have adapted a technique named proximity-dependent biotin identification (BioID) for use in T. gondii to identify novel components of the IMC. Using IMC proteins in both the alveoli and the cytoskeletal network as bait, we have uncovered a total of 19 new IMC proteins in both of these suborganellar compartments, two of which we functionally evaluate by gene knockout. Importantly, labeling of IMC proteins using this approach has revealed a group of proteins that localize to the sutures of the alveolar sacs that have been seen in their entirety in Toxoplasma species only by freeze fracture electron microscopy. Collectively, our study greatly expands the repertoire of known proteins in the IMC and experimentally validates BioID as a strategy for discovering novel constituents of specific cellular compartments of T. gondii. IMPORTANCE:The identification of binding partners is critical for determining protein function within cellular compartments. However, discovery of protein-protein interactions within membrane or cytoskeletal compartments is challenging, particularly for transient or unstable interactions that are often disrupted by experimental manipulation of these compartments. To circumvent these problems, we adapted an in vivo biotinylation technique called BioID for Toxoplasma species to identify binding partners and proximal proteins within native cellular environments. We used BioID to identify 19 novel proteins in the parasite IMC, an organelle consisting of fused membrane sacs and an underlying cytoskeleton, whose protein composition is largely unknown. We also demonstrate the power of BioID for targeted discovery of proteins within specific compartments, such as the IMC cytoskeleton. In addition, we uncovered a new group of proteins localizing to the alveolar sutures of the IMC. BioID promises to reveal new insights on protein constituents and interactions within cellular compartments of Toxoplasma

Coenzyme Q10 levels are low and may be associated with the inflammatory cascade in septic shock

Mitochondrial dysfunction is associated with increased mortality in septic shock. Coenzyme Q10 (CoQ10) is a key cofactor in the mitochondrial respiratory chain, but whether CoQ10 is depleted in septic shock remains unknown. Moreover, statin therapy may decrease CoQ10 levels, but whether this occurs acutely remains unknown. We measured CoQ10 levels in septic shock patients enrolled in a randomized trial of simvastatin versus placebo. We conducted a post hoc analysis of a prospective, randomized trial of simvastatin versus placebo in patients with septic shock (ClinicalTrials.gov ID: NCT00676897). Adult patients with suspected or confirmed infection and the need for vasopressor support were included in the initial trial. For the current analysis, blood specimens were analyzed for plasma CoQ10 and low-density lipoprotein (LDL) levels. The relationship between CoQ10 levels and inflammatory and vascular endothelial biomarkers was assessed using either the Pearson or Spearman correlation coefficient. We analyzed 28 samples from 14 patients. CoQ10 levels were low, with a median of 0.49 (interquartile range 0.26 to 0.62) compared to levels in healthy control patients (CoQ10 = 0.95 μmol/L ± 0.29; P < 0.0001). Statin therapy had no effect on plasma CoQ10 levels over time (P = 0.13). There was a statistically significant relationship between plasma CoQ10 levels and levels of vascular cell adhesion molecule (VCAM) (r2 = 0.2; P = 0.008), TNF-α (r2 = 0.28; P = 0.004), IL-8 (r2 = 0.21; P = 0.015), IL-10 (r2 = 0.18; P = 0.025), E-selectin (r2 = 0.17; P = -0.03), IL-1ra (r2 = 0.21; P = 0.014), IL-6 (r2 = 0.17; P = 0.029) and IL-2 (r2 = 0.23; P = 0.009). After adjusting for LDL levels, there was a statistically significant inverse relationship between plasma CoQ10 levels and levels of VCAM (r2 = 0.24; P = 0.01) (Figure 3) and IL-10 (r2 = 0.24; P = 0.02). CoQ10 levels are significantly lower in septic shock patients than in healthy controls. CoQ10 is negatively associated with vascular endothelial markers and inflammatory molecules, though this association diminishes after adjusting for LDL levels

Bubbling AdS Black Holes

We explore the non-BPS analog of `AdS bubbles', which are regular spherically symmetric 1/2 BPS geometries in type IIB supergravity. They have regular horizons and can be thought of as bubbling generalizations of non-extremal AdS black hole solutions in five-dimensional gauged supergravity. Due to the appearance of the Heun equation even at the linearized level, various approximation and numerical methods are needed in order to extract information about this system. We study how the vacuum expectation value and mass of a particular dimension two chiral primary operator depend on the temperature and chemical potential of the thermal Yang-Mills theory. In addition, the mass of the bubbling AdS black holes is computed. As is shown numerically, there are also non-BPS solitonic bubbles which are completely regular and arise from continuous deformations of BPS AdS bubbles.Comment: 37 pages, 2 figure

Optimal Trajectory and En-Route Contingency Planning for Urban Air Mobility Considering Battery Energy Levels

Copyright © 2022 by Seulki Kim, Caleb Harris, Cedric Y. Justin, and Dimitri Mavris . Published by the American Institute of Aeronautics and Astronautics, Inc., with permission.Presented at the AIAA Aviation Forum, June 27-July 1, 2022, Chicago, IL & VirtualUrban Air Mobility (UAM) is an electric propelled, vertical takeoff and landing (eVTOL) aircraft envisioned for transporting passengers and goods within metropolitan areas. Planning UAM flights will not be easy as unexpected wind turbulence from high-altitude structures may impact the vehicles operating at a low altitude. Furthermore, considering the short travel time of the UAM, smart and safe decision-making will be challenging, particularly in off-nominal situations that force the aircraft to divert to an alternate destination instead of landing at the initially planned destination. To overcome these challenges, this research proposes automated pre-flight and in-flight contingency planning systems to assist in both normal and irregular UAM operations. A planner in the pre-flight planning system optimizes an aerial trajectory between the scheduled origin and destination, avoiding restricted high-level structures and estimating energy levels. In the contingency planning system, an in-flight replanner produces several optimal trajectories from where the diversion is declared to each alternate destination candidate. A diversion decision-making tool then scores a list of candidates and selects the best site for diversion. Real-world operational scenarios in the city of Miami are presented to demonstrate the capability of the proposed framework

Anomalous scaling of a passive scalar advected by the Navier--Stokes velocity field: Two-loop approximation

The field theoretic renormalization group and operator product expansion are applied to the model of a passive scalar quantity advected by a non-Gaussian velocity field with finite correlation time. The velocity is governed by the Navier--Stokes equation, subject to an external random stirring force with the correlation function $\propto \delta(t-t') k^{4-d-2\epsilon}$. It is shown that the scalar field is intermittent already for small $\epsilon$, its structure functions display anomalous scaling behavior, and the corresponding exponents can be systematically calculated as series in $\epsilon$. The practical calculation is accomplished to order $\epsilon^{2}$ (two-loop approximation), including anisotropic sectors. Like for the well-known Kraichnan's rapid-change model, the anomalous scaling results from the existence in the model of composite fields (operators) with negative scaling dimensions, identified with the anomalous exponents. Thus the mechanism of the origin of anomalous scaling appears similar for the Gaussian model with zero correlation time and non-Gaussian model with finite correlation time. It should be emphasized that, in contrast to Gaussian velocity ensembles with finite correlation time, the model and the perturbation theory discussed here are manifestly Galilean covariant. The relevance of these results for the real passive advection, comparison with the Gaussian models and experiments are briefly discussed.Comment: 25 pages, 1 figur

Duality symmetry, strong coupling expansion and universal critical amplitudes in two-dimensional \Phi^{4} field models

We show that the exact beta-function \beta(g) in the continuous 2D g\Phi^{4} model possesses the Kramers-Wannier duality symmetry. The duality symmetry transformation \tilde{g}=d(g) such that \beta(d(g))=d'(g)\beta(g) is constructed and the approximate values of g^{*} computed from the duality equation d(g^{*})=g^{*} are shown to agree with the available numerical results. The calculation of the beta-function \beta(g) for the 2D scalar g\Phi^{4} field theory based on the strong coupling expansion is developed and the expansion of \beta(g) in powers of g^{-1} is obtained up to order g^{-8}. The numerical values calculated for the renormalized coupling constant g_{+}^{*} are in reasonable good agreement with the best modern estimates recently obtained from the high-temperature series expansion and with those known from the perturbative four-loop renormalization-group calculations. The application of Cardy's theorem for calculating the renormalized isothermal coupling constant g_{c} of the 2D Ising model and the related universal critical amplitudes is also discussed.Comment: 16 pages, REVTeX, to be published in J.Phys.A:Math.Ge

Anomalous scaling of passively advected magnetic field in the presence of strong anisotropy

Inertial-range scaling behavior of high-order (up to order N=51) structure functions of a passively advected vector field has been analyzed in the framework of the rapid-change model with strong small-scale anisotropy with the aid of the renormalization group and the operator-product expansion. It has been shown that in inertial range the leading terms of the structure functions are coordinate independent, but powerlike corrections appear with the same anomalous scaling exponents as for the passively advected scalar field. These exponents depend on anisotropy parameters in such a way that a specific hierarchy related to the degree of anisotropy is observed. Deviations from power-law behavior like oscillations or logarithmic behavior in the corrections to structure functions have not been found.Comment: 15 pages, 18 figure