Chagas disease in the Brazilian Amazon: IV. a new cross-sectional study

A new conglomerate family sample of 194 dwellings with 996 resident persons were studied in the town of Barcelos, State of Amazonas, in order to re-evaluate the risk of Chagas disease. During the survey the persons were interviewed and in this occasion we showed to them a collection of Panstrongylus, Rhodnius and Triatoma, asking if they recognized and eventually have been bitten by this kind of bugs. At this time we collected 500 ul of blood in microtainer® tubes from 886 interviewed persons who gave permission after informed consent. A screening test for T. cruzi antibodies based on agglutination of colored polymer particles, sensitized with three different synthetic peptides of T. cruzi (ID-PaGIA Chagas Test)®, showed 13.2% of sera positivity, but only 6.8% were confirmed by indirect immunofluorescence, and ELISA with purified T. cruzi antigens. Two hundred and six interviewed persons (20.7%) recognized the triatomines, as "piaçavas' lice" and 62 (30%) confirmed that have been bitten by the bugs, 25.8% of them had a positive serology for T. cruzi infection. Electrocardiographic alterations were shown in 9.3% of the seropositives and in 11.9% of the seronegative cases. This was considered not statistically significant

Chagas disease in the Amazon Region

Submitted by Patricia Stilpen ([email protected]) on 2011-04-22T20:55:02Z No. of bitstreams: 1 Chagas disease in the Amazon Region.pdf: 505204 bytes, checksum: 2c95c1c6ed6c4bcb7315f7fb059308b1 (MD5)Made available in DSpace on 2011-04-22T20:55:02Z (GMT). No. of bitstreams: 1 Chagas disease in the Amazon Region.pdf: 505204 bytes, checksum: 2c95c1c6ed6c4bcb7315f7fb059308b1 (MD5) Previous issue date: 2007Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.The risk that Chagas disease becomes established as a major endemic threat in Amazonia (the world´s largest tropical biome, today inhabited by over 30 million people) relates to a complex set of interacting biological and social determinants. These include intense immigration from endemic areas (possibly introducing parasites and vectors), extensive landscape transformation with uncontrolled deforestation, and the great diversity of wild Trypanosoma cruzi reservoir hosts and vectors (25 species in nine genera), which maintain intense sylvatic transmission cycles. Invasion of houses by adventitious vectors (with infection rates > 60%) is common, and focal adaptation of native triatomines to artificial structures has been reported. Both acute (~ 500) and chronic cases of autochthonous human Chagas disease have been documented beyond doubt in the region. Continuous, low-intensity transmission seems to occur throughout the Amazon, and generates a hypoendemic pattern with seropositivity rates of ~ 1-3%. Discrete foci also exist in which transmission is more intense (e.g., in localized outbreaks probably linked to oral transmission) and prevalence rates higher. Early detection-treatment of acute cases is crucial for avoiding further dispersion of endemic transmission of Chagas disease in Amazonia, and will require the involvement of malaria control and primary health care systems. Comprehensive eco-epidemiological research, including prevalence surveys or the characterization of transmission dynamics in different ecological settings, is still needed. The International Initiative for Chagas Disesae Surveillance and Prevention in the Amazon provides the framework for building up the political and scientific cooperation networks required to confront the challenge of preventing Chagas disease in Amazonia

Chagas disease in the Amazon Region

The risk that Chagas disease becomes established as a major endemic threat in Amazonia (the world's largest tropical biome, today inhabited by over 30 million people) relates to a complex set of interacting biological and social determinants. These include intense immigration from endemic areas (possibly introducing parasites and vectors), extensive landscape transformation with uncontrolled deforestation, and the great diversity of wild Trypanosoma cruzi reservoir hosts and vectors (25 species in nine genera), which maintain intense sylvatic transmission cycles. Invasion of houses by adventitious vectors (with infection rates > 60%) is common, and focal adaptation of native triatomines to artificial structures has been reported. Both acute (~ 500) and chronic cases of autochthonous human Chagas disease have been documented beyond doubt in the region. Continuous, low-intensity transmission seems to occur throughout the Amazon, and generates a hypoendemic pattern with seropositivity rates of ~ 1-3%. Discrete foci also exist in which transmission is more intense (e.g., in localized outbreaks probably linked to oral transmission) and prevalence rates higher. Early detection-treatment of acute cases is crucial for avoiding further dispersion of endemic transmission of Chagas disease in Amazonia, and will require the involvement of malaria control and primary health care systems. Comprehensive eco-epidemiological research, including prevalence surveys or the characterization of transmission dynamics in different ecological settings, is still needed. The International Initiative for Chagas Disesae Surveillance and Prevention in the Amazon provides the framework for building up the political and scientific cooperation networks required to confront the challenge of preventing Chagas disease in Amazonia

Chagas Disease in the Brazilian Amazon. IV. A new cross-sectional study

Submitted by Sandra Infurna ([email protected]) on 2019-07-11T17:03:37Z No. of bitstreams: 1 AngelaJUnqueira_JRCoura_etal_IOC_2002.pdf: 264038 bytes, checksum: be657278417078927e24a9d3ea19027a (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2019-07-11T17:14:25Z (GMT) No. of bitstreams: 1 AngelaJUnqueira_JRCoura_etal_IOC_2002.pdf: 264038 bytes, checksum: be657278417078927e24a9d3ea19027a (MD5)Made available in DSpace on 2019-07-11T17:14:25Z (GMT). No. of bitstreams: 1 AngelaJUnqueira_JRCoura_etal_IOC_2002.pdf: 264038 bytes, checksum: be657278417078927e24a9d3ea19027a (MD5) Previous issue date: 2002Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil / Universidade do Estado do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil / Universidade do Estado do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Estudantes de Pós-Graduação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Estudantes de Pós-Graduação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Estudantes de Pós-Graduação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Estudantes de Pós-Graduação. Rio de Janeiro, RJ, Brasil.A new conglomerate family sample of 194 dwellings with 996 resident persons were studied in the town of Barcelos, State of Amazonas, in order to re-evaluate the risk of Chagas disease. During the survey the persons were interviewed and in this occasion we showed to them a collection of Panstrongylus, Rhodnius and Triatoma, asking if they recognized and eventually have been bitten by this kind of bugs. At this time we collected 500 ul of blood in microtainer tubes from 886 interviewed persons who gave permission after informed consent. A screening test for T. cruzi antibodies based on agglutination of colored polymer particles, sensitized with three different synthetic peptides of T. cruzi (ID-PaGIA Chagas Test) , showed 13.2% of sera positivity, but only 6.8% were confirmed by indirect immunofluorescence, and ELISA with purified T. cruzi antigens. Two hundred and six interviewed persons (20.7%) recognized the triatomines, as “piaçavas’ lice” and 62 (30%) confirmed that have been bitten by the bugs, 25.8% of them had a positive serology for T. cruzi infection. Electrocardiographic alterations were shown in 9.3% of the seropositives and in 11.9% of the seronegative cases. This was considered not statistically significant

Morbidade da doença de Chagas em áreas do Sertão da Paraíba e da Caatinga do Piauí

Foram estudados 186 pares de indivíduos sorologicamente positivos e negativos para infecção chagásica, da mesma idade e sexo, do Sertão da Paraíba e 200 indivíduos também sorologicamente positivos nos municípios de Oeiras e Colônia do Piauí. Depois de confirmados por pelo menos dois outros testes sorológicos: imunofluorescência indireta quantitativa, ELISA, hemaglutinação ou fixação do complemento,foi feito o exame clínico, eletrocardiográfico e radiológico nos indivíduos selecionados para o estudo exenodiagnóstico, hemocultura e PCR em amostras representativas dos casos soropositivos. As manifestações clínicas predominantes entre os soropositivos em ambas as áreas foram palpitações, dispnéia aos esforços, disfagia, odinofagia, pirose e obstipação. As freqüências das alterações eletrocardiográficas sugestivas da doença de Chagas foram, respectivamente, na Paraíba e no Piauí: BAV = 3,8 % e 2 %, BRD III = 6,4 % e 7 %, BRD III + HBAE = 10,7 % e 10,5 % e extra-sístoles ventriculares complexas = 2,7 % e 3,% . O xenodiagnóstico foi positivo em 13 % dos casos soropositivos da Paraíba e em 34 % dos casos do Piauí, enquanto que o PCR foi positivo, respectivamente, em 44,6 e 59,5 %. A hemocultura realizada apenas no Piauí foi positiva em 25,7 % dos casos estudados. Foram realizados inquéritos triatomínicos em 132 domicílios e peridomicílios no Sertão da Paraíba e em 159 na Caatinga do Piauí, sendo capturados 16 exemplares de T. brasiliensis não infectados no peridomicílio na Paraíba e 750 exemplares no Piauí, dos quais 625 foram examinados: 49 de T. pseudomaculata não infectados com T. cruzi (19 no intradomicílio e 30 no peridomicílio) e 576 de T. brasiliensis (371 no intradomicílio e 205 no peridomicílio) entre os quais 32 (5,5% ) estavam infectados com T. cruzi (31 no intradomicílio e um no peridomicílio)

Reativação da infecção por Trypanosoma cruzi em paciente com síndrome de imunodeficiência adquirida

Uma paciente com síndrome de imunodeficiência adquirida (SIDA) e doença de Chagas, com xenodiagnóstico positivo, estava em uso prolongado de cetoconazol com o objetivo de suprimir a parasitemia e prevenir a reativação da doença de Chagas. O cetoconazol foi suspenso inadvertidamente após 6 meses de uso. Um mês após, a paciente foi internada com febre, cefaléia, vômitos, taquicardia e hepatoesplenomegalia. Tanto o xenodiagnóstico como o exame de sangue a fresco demonstraram a presença de Trypanosoma cruzi. O tratamento com benzonidazol foi instituído, com supressão da parasitemia. A paciente desenvolveu concomitantemente uma provável neurotoxoplasmose, evoluindo para o óbito em septicemia. À necropsia, não foram encontrados parasitas

Human acute Chagas disease: changes in factor VII, activated protein C and hepatic enzymes from patients of oral outbreaks in Pará State (Brazilian Amazon)

Submitted by Sandra Infurna ([email protected]) on 2020-03-20T17:47:24Z No. of bitstreams: 1 ValeriaSantos_WIlsonsavino_etal_IOC_2020.pdf: 734780 bytes, checksum: 1d518be2fd762a884afef96fc9df49dc (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2020-03-20T18:01:26Z (GMT) No. of bitstreams: 1 ValeriaSantos_WIlsonsavino_etal_IOC_2020.pdf: 734780 bytes, checksum: 1d518be2fd762a884afef96fc9df49dc (MD5)Made available in DSpace on 2020-03-20T18:01:26Z (GMT). No. of bitstreams: 1 ValeriaSantos_WIlsonsavino_etal_IOC_2020.pdf: 734780 bytes, checksum: 1d518be2fd762a884afef96fc9df49dc (MD5) Previous issue date: 2020Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ, Brasil / Secretaria de Saúde Pública do Estado do Pará. Belém, PA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação, Rio de Janeiro, RJ, Brasil.Universidade Federal do Pará. Faculdade de Medicina. Belém, PA, Brasil.Fundação Oswaldo Cruz-Fiocruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro; Departamento de Estatística. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação, Rio de Janeiro, RJ, Brasil.Secretaria de Saúde Pública do Estado do Pará. Belém, PA, Brasil.nstituto Nacional de Câncer. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Doenças Parasitárias. Rio de Janeiro, RJ, Brasil..Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação, Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Doenças Parasitárias. Rio de Janeiro, RJ, Brasil..Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação, Rio de Janeiro, RJ, Brasil.Oral transmission of Chagas disease has been increasing in Latin American countries. The present study aimed to investigate changes in hepatic function, coagulation factor levels and parasite load in human acute Chagas disease (ACD) secondary to oral Trypanosoma cruzi transmission. Clinical and epidemiological findings of 102 infected individuals attended in the State of Pará from October 2013 to February 2016 were included. The most common symptoms were fever (98%), asthenia (83.3%), face and limb edema (80.4%), headache (74.5%) and myalgia (72.5%). The hepatic enzymes alanine aminotransferase (ALT) and aspartate aminotransferase (AST) of 30 ACD patients were higher compared with controls, and this increase was independent of the treatment with benznidazole. Moreover, ACD individuals had higher plasma levels of activated protein C and lower levels of factor VII of the coagulation cascade. Patients with the highest parasite load had also the most increased transaminase levels. Also, ALT and AST were associated moderately (r = 0.429) and strongly (r = 0.595) with parasite load respectively. In conclusion, the present study raises the possibility that a disturbance in coagulation and hepatic function may be linked to human ACD