Summary of dermoscopic features, reflectance confocal microscopy and histopathologic aspects.

<p>Summary of dermoscopic features, reflectance confocal microscopy and histopathologic aspects.</p

Superficial spreading melanoma, Breslow thickness 0,79 mm.

<p>This lesion shows on dermoscopy (A) pseudopods (white circle corresponds to the punch area). RCM mosaic image (B, 3×3 mm) at the level of the DEJ shows compact aggregates of atypical cells distributed in a linear arrangement toward the periphery with a dense nest at the extremity (the area inside the dashed square is represented in figure C). RCM individual image (C, 0,5×0,5 mm) at the level of the DEJ shows a pseudopod in detail, characterized by elongated, dense and bright peripheral aggregate. Perpendicular section (D) shows nests of atypical melanocytes distributed contiguously toward the periphery along the DEJ. Transverse section (E) shows nests of atypical cells arranged in a linear manner throughout the periphery of the lesion (black arrows).</p

Superficial spreading melanoma <i>in situ</i>.

<p>This lesion shows on dermoscopy (A) a slightly pigmented network (white circle corresponds to the punch area). RCM mosaic image (B, 1×1 mm) at the level of the DEJ shows irregular and dishomogeneous dermal papillae with dendritic cells (white arrows). RCM individual image (C, 0,5×0,5 mm) at the level of the DEJ shows dendritic cells forming “bridges” called mitochondria-like structures (white arrow). Perpendicular section (D) shows disarrangement of the rete ridge and the increased number of atypical melanocytes. Transverse section (E, HE staining) shows atypical melanocytes protruding into the dermal papillae forming bridges (black arrows). Transverse section (F, Melan-A staining) shows cells positive for Melan-A protruding into the dermal papillae (white arrows).</p

Melanocytic Nevi.

<p>These lesions show typical network (A) and regular globules (D), on dermoscopy. RCM mosaic image (B, 1×1 mm) at the level of the DEJ shows rings of bright polygonal cells surrounding roundish to oval dark areas corresponding to dermal papillae at DEJ. Transverse section (C) shows isolated melanocytes arranged around the dermal papillae and there are nevus cells nests within the epidermis. RCM mosaic image (E, 1,5×1,5 mm) at the level of the DEJ and dermis shows compact aggregates of large polygonal cells similar in morphologic features and reflectivity, forming polyhedral structures. Transverse section (F) shows dense nests composed of nevus cells within the dermis surrounded by a narrow band of epidermis.</p

Superficial spreading melanoma <i>in situ</i>.

<p>This lesion shows on dermoscopy (A) a broadened pigmented network (white circle corresponds to the punch area). RCM mosaic images (B and D, 1×1 mm) at the level of the DEJ show demarcated and non-demarcated rings separated by loosely thick interpappilary spaces (white arrows) and some plump bright cells and bright dots are visible within dermal papillae (arrowheads). Perpendicular section (C) shows disarrangement of the rete ridge and the increased number of atypical melanocytes in the epidermis. Transverse section (E) shows predominance of atypical melanocytes, isolated or in nests, enlarging the interpapillary spaces (black arrow).</p

Distribution of cases according to BRAF V600E status and number of cumulative predictive factors.

<p>Distribution of cases according to BRAF V600E status and number of cumulative predictive factors.</p

UAC_PONE-D-17-07514_RCM of BRAF V600E mutated thin melanomas detected IHQ statistical analyses

Statistical analysis of data presented in the paper Reflectance confocal microscopy features of BRAF V600E mutated thin melanomas detected by immunohistochemistr

UAC_Tables 1 to 7_PONE-D-17-07514_RCM of BRAF V600E mutated thin melanomas detected IHQ

All tables (1 to 7) in the article Reflectance confocal microscopy features of BRAF V600E mutated thin melanomas detected by immunohistochemistr

Graphic age x <i>BRAF</i> V600E.

<p>Graphic age x <i>BRAF</i> V600E.</p

Comparison between findings of dermatoscopy and RCM.

<p>(A) Dermoscopy of <i>BRAF</i> V600E negative <i>in situ</i> superficial spreading melanoma: broadened pigmented network (the inset corresponds to clinical image). (B) RCM mosaic image (1.5x1.5 mm) in DEJ shows junctional thickening due to isolated atypical cells (yellow arrows). (C) RCM mosaic image (1.5x1.5 mm) in DEJ showing non-edged papillae separated by loosely thick interpapillary spaces (yellow arrows) and meshwork pattern (red arrows). (D) Dermoscopy of <i>BRAF</i> V600E mutated superficial spreading melanoma, 1 mm in Breslow thickness): multicomponent pattern (the inset corresponds to clinical image). (E) RCM mosaic image (0.75x0.75 mm) at the level of the epidermis shows hyporeflective pagetoid cells (red arrows) and epidermal nests (yellow arrow). (F) RCM mosaic image (0.75x0.75 mm) at the level of the DEJ shows dermal-epidermal nests (yellow arrows).</p