Cy_Gsnap-alignment

Sam files of the 38 individuals with two types of alignment to a Pseudo-Reference-Genome (PRG), to the American Crow (Cy_AmericanCrow) and the Zebra Finch (Cy_ZebraFinch), performed with Gsnap and after all filters (clone filter, optical duplicates and mapping quality)

Cy_STACKS_output

STACKS output from the correction module (rx_stacks) of ref_map (two PRG: PRG_AmericanCrow and PRG_ZebraFinch) and de_novo (run 1 and run 2 separate: Denovo_R1 and Denovo_R2) pipelines and populations maps used (Cy_2POP.txt and Cy_3POP.txt). Included are the catalog.tags, de-novo assemblies and output of the populations module. Each directory includes summary statistics per locus and overall, and all SNP data in STRUCTURE, FASTA, Genpop, PLINK and VCF format

Cy_Fasta_BLAST

FASTA file of consensus sequences containing all SNPs (CLOCUS_) used in the study and BLAST (MATCHCLOCUS and Q_Query=) results of these loci

Cy_FASTQ

FASTq sequences of 38 individuals used, with their corresponding barcode from the sequencing. See file "Cy_GENERAL-DATA-ID-GEO-CATALOG.txt" to know the general information of the samples

Cy_Baypass_input

Input used in BayPass analyses including genetic (Cy.BayPass2allele.count.txt) and environmental (Cy.BayPass-var-X.txt) data. The environmental data are in separate files for each of the final nine climatic variables used (e.g Cy.BayPass-var-bio4.txt). The order of the samples are in file "ORDER-SAMPLES-BayPass.txt"

Cy_GENERAL-DATA-ID-GEO-CATALOG

General information of the 38 samples: catalog-voucher from the National Ornithology Collection (UNAM, Mexico), sample ID (barcodes from the sequencing) geographic position (longitude and latitude), the corresponding operative-geogaraphic-unit (OGU), vegetation type and number of the sampling point (see Figure 1 in the article)

Cy_LFMM_input

Input used in LFMM analyses including genetic (Cy.lfmm) and environmental (Cy.lfmm.env) data for each individual. The order of the samples are in file "ORDER-SAMPLES-plink-LFMM.txt

Data_Sheet_1.PDF

<p>Analyses of genetic variation allow understanding the origin, diversification and genetic resources of cultivated plants. Domesticated taxa and their wild relatives are ideal systems for studying genetic processes of plant domestication and their joint is important to evaluate the distribution of their genetic resources. Such is the case of the domesticated subspecies C. argyrosperma ssp. argyrosperma, known in Mexico as calabaza pipiana, and its wild relative C. argyrosperma ssp. sororia. The main aim of this study was to use molecular data (microsatellites) to assess the levels of genetic variation and genetic differentiation within and among populations of domesticated argyrosperma across its distribution in Mexico in comparison to its wild relative, sororia, and to identify environmental suitability in previously proposed centers of domestication. We analyzed nine unlinked nuclear microsatellite loci to assess levels of diversity and distribution of genetic variation within and among populations in 440 individuals from 19 populations of cultivated landraces of argyrosperma and from six wild populations of sororia, in order to conduct a first systematic analysis of their genetic resources. We also used species distribution models (SDMs) for sororia to identify changes in this wild subspecies’ distribution from the Holocene (∼6,000 years ago) to the present, and to assess the presence of suitable environmental conditions in previously proposed domestication sites. Genetic variation was similar among subspecies (H_E = 0.428 in sororia, and H_E = 0.410 in argyrosperma). Nine argyrosperma populations showed significant levels of inbreeding. Both subspecies are well differentiated, and genetic differentiation (F_ST) among populations within each subspecies ranged from 0.152 to 0.652. Within argyrosperma we found three genetic groups (Northern Mexico, Yucatan Peninsula, including Michoacan and Veracruz, and Pacific coast plus Durango). We detected low levels of gene flow among populations at a regional scale (<0.01), except for the Yucatan Peninsula, and the northern portion of the Pacific Coast. Our analyses suggested that the Isthmus of Tehuantepec is an effective barrier isolating southern populations. Our SDM results indicate that environmental characteristics in the Balsas-Jalisco region, a potential center of domestication, were suitable for the presence of sororia during the Holocene.</p