Expansion of the ligand knowledge base for chelating P,P-donor ligands (LKB-PP)

[Image: see text] We have expanded the ligand knowledge base for bidentate P,P- and P,N-donor ligands (LKB-PP, Organometallics2008, 27, 1372–1383) by 208 ligands and introduced an additional steric descriptor (nHe(8)). This expanded knowledge base now captures information on 334 bidentate ligands and has been processed with principal component analysis (PCA) of the descriptors to produce a detailed map of bidentate ligand space, which better captures ligand variation and has been used for the analysis of ligand properties

Prevalence and antimicrobial resistance of MRSA across different pig age groups in an intensive pig production system in Australia

This observational study aimed to determine MRSA prevalence using strain‐specific real‐time PCR at the pig level, stratified by age groupings, within a pig enterprise. A total of 658 samples were collected from individual pigs (n = 618) and the piggery environment (n = 40), distributed amongst five different pig age groups. Presumptive MRSA isolates were confirmed by the presence of mecA , and MALDI‐TOF was performed for species verification. All isolates were tested against 18 different antimicrobials. MRSA was isolated from 75.2% (95% CI 71.8–78.6) of samples collected from pigs, and 71% of the MRSA isolates from this source were identified as community‐associated (CA)‐MRSA ST93, while the remainder were livestock‐associated (LA)‐MRSA ST398. Amongst environmental isolates, 80% (CI 64.3–95.7) were ST93 and the remainder ST398. All MRSA isolates from pigs and the environment were susceptible to ciprofloxacin, gentamicin, linezolid, mupirocin, rifampicin, sulfamethoxazole–trimethoprim, teicoplanin and vancomycin. Phenotypic rates of resistance were penicillin (100%), clindamycin (97.6%), erythromycin (96.3%), ceftiofur (93.7%), chloramphenicol (81.2%), tetracycline (63.1%) and amoxicillin–clavulanate (63.9%). A low prevalence of resistance (9.2%) was observed against neomycin and quinupristin–dalfopristin. The probability of MRSA carriage in dry sows (42.2%) was found to be significantly lower (p < .001) when compared to other age groups: farrowing sows (76.8%, RR1.82), weaners (97.8%, RR 2.32), growers (94.2%, RR 2.23) and finishers (98.3%, RR 2.33). Amongst different production age groups, a significant difference was also found in antimicrobial resistance for amoxicillin–clavulanate, neomycin, chloramphenicol and tetracycline. Using the RT‐PCR assay adopted in this study, filtering of highly prevalent ST93 and non‐ST93 isolates was performed at high throughput and low cost. In conclusion, this study found that weaner pigs presented a higher risk for CA‐MRSA and antimicrobial resistance compared to other age groups. These findings have major implications for how investigations of MRSA outbreaks should be approached under the One‐Health context

Validation of the approach method of electropherogram by a sum of gaussians

Quantitative estimation of one dimensionnal autoradiography film density profiles that are generated in gel electrophoresis of radioactive proteins and nucleic acids is a very used approach . Several studies have been realized on this subject . They operate on the optical density values acquired by CCD cameras . These operations include smoothing of mesuring values, background correction, and integration of peak areas after sided fit of half gaussian profile to each band . Our contribution consists to validate this approach by its application to separate RNA fragments of very close size. We show that is possible to use this approach to evaluate directly the relative expression level of each RNA in a determined experiance configuration .L'estimation quantitative des autoradiogrammes par une somme de gaussiennes est une approche très utilisée. Elle est rendue possible grâce au développement des méthodes densimétriques utilisables après digitalisation de l'image d'autoradiographie du gel. La métrologie apportée par l'analyse et le traitement d'images permet d'éliminer les appréciations subjectives visuelles et humaines. Plusieurs études ont été réalisées sur ce sujet. Notre contribution consiste à valider cet approche par son application à des fragments d'ARN de tailles très proches. Nous montrons qu'il est possible d'évaluer directement par le calcul le niveau d'expression d'ARN dans une configuration d'expérience déterminée, évaluation très difficile à obtenir par d'autres méthodes

Deformaciones estructurales de la iglesia visigoda de Sant Miquel de Terrassa

The paper presents the detailed analysis of the current state of structural deformations of a Visigothic funerary church with a square floor plan and an “inscribed” Greek cross is made. The laser scanner documentation constitutes the metric base that facilitates the identification of the variations suffered by the factory based on an optimal and theoretical stage of the original building. The results allow us to deepen in the understanding of the historical evolution of the building, as well as to establish a comparative point for future structural monitoring actions.El artículo presenta el análisis detallado del estado actual de las deformaciones estructurales de una iglesia funeraria visigoda de planta cuadrada con una cruz griega inscrita. La documentación efectuada con el escáner láser constituye la base métrica que facilita la identificación de las variaciones sufridas por la fábrica en base a un estadio óptimo y teórico de la edificación original. Los resultados permiten profundizar en la comprensión sobre la evolución histórica del edificio, así como establecer un punto comparativo para futuras acciones de seguimiento estructural

A Multi-APD readout for EL detectors

Detectors with an electroluminesence readout show an excellence performance in respect of energy resolution making them interesting for various applications as X-ray detection, double beta and dark matter experiments, Compton and gamma cameras, etc. In the following the study of a readout based on avalanche photo diodes to detect directly the VUV photons is presented. Results of measurements with 5 APDs in xenon at pressures between 1 and 1.65 bar are shown indicating that such a readout can provide excellent energy and a moderate position resolution.Comment: Talk given at the 5th Symposium on Large TPCs for Low Energy Rare Events. Submitted to JPCS (Journal of Physics: Conference Series) for publication. 8 pages, 17 figure

Desarrollo de subsistemas para la medida de parámetros de ruido

Peer ReviewedPostprint (published version

Reflecting on One Health in Action During the COVID-19 Response

The COVID-19 pandemic, a singular disruptive event in recent human history, has required rapid, innovative, coordinated and collaborative approaches to manage and ameliorate its worst impacts. However, the threat remains, and learning from initial efforts may benefit the response management in the future. One Health approaches to managing health challenges through multi-stakeholder engagement are underscored by an enabling environment. Here we describe three case studies from state (New South Wales, Australia), national (Ireland), and international (sub-Saharan Africa) scales which illustrate different aspects of One Health in action in response to the COVID-19 pandemic. In Ireland, a One Health team was assembled to help parameterise complex mathematical and resource models. In New South Wales, state authorities engaged collaboratively with animal health veterinarians and epidemiologists to leverage disease outbreak knowledge, expertise and technical and support structures for application to the COVID-19 emergency. The African One Health University Network linked members from health institutions and universities from eight countries to provide a virtual platform knowledge exchange on COVID-19 to support the response. Themes common to successful experiences included a shared resource base, interdisciplinary engagement, communication network strategies, and looking global to address local need. The One Health approaches used, particularly shared responsibility and knowledge integration, are benefiting the management of this pandemic and future One Health global challenges

Guía de práctica clínica para la elección del fluido de restauración volémica perioperatoria en los pacientes adultos intervenidos de cirugía no cardiaca

Esta Guía de Práctica Clínica responde a preguntas clínicas sobre seguridad en la elección de fluido (cristaloide, coloide o Hidroxietilalmidón 130) en pacientes que precisan restauración volémica en el periodo perioperatorio de cirugía no cardiaca. A partir del resumen de la evidencia, se elaboraron las recomendaciones siguiendo la metodología GRADE. En esta población se sugiere la fluidoterapia basada en la administración de cristaloides, (recomendación débil, calidad de la evidencia baja). En las situaciones en las que la restauración volémica no se alcance sólo con cristaloides, se sugiere utilizar coloides sintéticos (Hidroxietilalmidón 130 o gelatina fluida modificada) en lugar de Albúmina 5% (recomendación débil, calidad de la evidencia baja). La elección y dosificación de coloide deberán basarse en las características del producto, comorbilidad del paciente y experiencia del anestesiólogo