Determinants biològics i fisiològics del Pagre (Pagrus pagrus). Punts claus per al seu cultiu intensiu

The sparid teleost red porgy (PagntS pagn¡s) is an species with increasing potential and interest in the commercial aquaculture of the Mediterranean area. Although many of the aspects concerning its reproduction and nutrition are not well known, spontaneous spawning has been obtained in fish farms and growth rates obtained with commercial food are similar to those of sea bass and sea bream. Concerning culture conditions and stress influences, the results seem to suggest that red porgy shows a lower degree of adaptation compared to other Mediterranean sparids

Diferències morfomètriques i merístiques entre l'orada cultivada i salvatge

Gilthead seabream is of high commercíal interest and has become an important aquacultural resource in the Mediterranean countries. Now, the aim of improving the quality of reared products in order to meet markets preferences of conformity with wild standards is a new priority, The aim of this study is the use of morphometrics and meristics for the quantification of the differences in quality assessment and on their causative factors between and within sea bream reared in different fish farms and wild standards. Reared S.auratus show higher body depth, caudal peduncle depth, last dorsal spine, anal fin rays number and position of first anal spine respect to dorsal, and lower head depth, snout length, preorbital length, predorsal length, dorsal fin base, pectoral fin length, ventral fin length, caudal peduncle length, scales berween lateral line and dorsal, pectoral fin rays number and largest dorsal fin spine than wild sea bream. Apart of this measures, Cupimar hatchery facility has fish with lower head length, interorbital length and distance between pelvic and pectoral fins than wild sea bream; Maresa hatchery físhes have lower anal fin base and higher head length than wild standards, Blanes Peix sea cages show lower head length, orbital diameter and interorbital length than wild standards; and Aquadelt fishes show lower head length, orbital diameter, interorbital length, distance berween pelvic and pectoral fins and preanal length than wild sea bream and a non significant difference in last dorsal spine

Pth reloaded: a new evolutionary perspective

The parathyroid hormone (PTH) family is a group of structurally-related secreted peptides involved in bone mineral homeostasis and multitude of developmental processes in vertebrates. These peptides mediate actions through PTH receptors (PTHRs), which belong to the transmembrane G protein-coupled receptor group. To date, genes encoding for PTH and PTHR have only been identified in chordates, suggesting that this signaling pathway may be an evolutionary innovation of our phylum. In vertebrates, we found up to six PTH and three PTHR different paralogs, varying in number between mammals and teleost fishes due to the different rounds of whole-genome duplication and specific gene losses suffered between the two groups of animals. The diversification of the PTH gene family has been accompanied by both functional divergence and convergence, making sometimes difficult the comparison between PTH peptides of teleosts and mammals. Here, we review the roles of all Pth peptides in fishes, and based on the evolutionary history of PTH paralogs, we propose a new and simple nomenclature from PTH1 to PTH4. Moreover, the recent characterization of the Pth4 in zebrafish allows us to consider the prominent role of the brain-to-bone signaling pathway in the regulation of bone development and homeostasis. Finally, comparison between PTH peptides of fish and mammals allows us to discuss an evolutionary model for PTH functions related to bone mineral balance during the vertebrate transition from an aquatic to a terrestrial environment

Functional analysis of new human Bardet-Biedl syndrome loci specific variants in the zebrafish model

The multiple genetic approaches available for molecular diagnosis of human diseases have made possible to identify an increasing number of pathogenic genetic changes, particularly with the advent of next generation sequencing (NGS) technologies. However, the main challenge lies in the interpretation of their functional impact, which has resulted in the widespread use of animal models. We describe here the functional modelling of seven BBS loci variants, most of them novel, in zebrafish embryos to validate their in silico prediction of pathogenicity. We show that target knockdown (KD) of known BBS (BBS1, BB5 or BBS6) loci leads to developmental defects commonly associated with ciliopathies, as previously described. These KD pleiotropic phenotypes were rescued by co-injecting human wild type (WT) loci sequence but not with the equivalent mutated mRNAs, providing evidence of the pathogenic effect of these BBS changes. Furthermore, direct assessment of cilia located in Kupffer’s vesicle (KV) showed a reduction of ciliary length associated with all the studied variants, thus confirming a deleterious effect. Taken together, our results seem to prove the pathogenicity of the already classified and unclassified new BBS variants, as well as highlight the usefulness of zebrafish as an animal model for in vivo assays in human ciliopathiesMinisterio de Economía y Competitividad | Ref. AGL2017-89648