Caffeine blocks disruption of blood brain barrier in a rabbit model of Alzheimer's disease-4

) at the sites where IgG extravasation (red) was present. These effects were blocked by chronic ingestion of caffeine. Representative images taken from 2 rabbits in each group with 6 sections from each animal are shown. Bar = 20 μm.<p><b>Copyright information:</b></p><p>Taken from "Caffeine blocks disruption of blood brain barrier in a rabbit model of Alzheimer's disease"</p><p>http://www.jneuroinflammation.com/content/5/1/12</p><p>Journal of Neuroinflammation 2008;5():12-12.</p><p>Published online 3 Apr 2008</p><p>PMCID:PMC2330033.</p><p></p

Caffeine blocks disruption of blood brain barrier in a rabbit model of Alzheimer's disease-2

Ulb (A), cerebral cortex (B) and hippocampus (C), and these effects were blocked by caffeine at the dose of 3 mg/day. n = 2, *p < 0.05.<p><b>Copyright information:</b></p><p>Taken from "Caffeine blocks disruption of blood brain barrier in a rabbit model of Alzheimer's disease"</p><p>http://www.jneuroinflammation.com/content/5/1/12</p><p>Journal of Neuroinflammation 2008;5():12-12.</p><p>Published online 3 Apr 2008</p><p>PMCID:PMC2330033.</p><p></p

Caffeine blocks disruption of blood brain barrier in a rabbit model of Alzheimer's disease-5

the sites where IgG extravasation (red) was present. These effects were blocked by chronic ingestion of caffeine. Representative images taken from 2 rabbits in each group with 6 sections from each animal are shown. Bar = 20 μm.<p><b>Copyright information:</b></p><p>Taken from "Caffeine blocks disruption of blood brain barrier in a rabbit model of Alzheimer's disease"</p><p>http://www.jneuroinflammation.com/content/5/1/12</p><p>Journal of Neuroinflammation 2008;5():12-12.</p><p>Published online 3 Apr 2008</p><p>PMCID:PMC2330033.</p><p></p

Caffeine blocks disruption of blood brain barrier in a rabbit model of Alzheimer's disease-9

His effect was blocked by caffeine at the dose of 3 mg/day. Caffeine alone had no effect on extravasation of fibrinogen in normal rabbit brain. Representative images taken from 2 rabbits in each group with 6 sections from each animal are shown. Bar = 50 μm. (B) Cholesterol-enriched diet significantly increased accumulation of fibrinogen in olfactory bulb and this effect was blocked by caffeine at the dose of 3 mg/day. (C) Neither cholesterol-enriched diet nor caffeine changed significantly plasma levels of fibrinogen. n = 4, *p < 0.05.<p><b>Copyright information:</b></p><p>Taken from "Caffeine blocks disruption of blood brain barrier in a rabbit model of Alzheimer's disease"</p><p>http://www.jneuroinflammation.com/content/5/1/12</p><p>Journal of Neuroinflammation 2008;5():12-12.</p><p>Published online 3 Apr 2008</p><p>PMCID:PMC2330033.</p><p></p

Caffeine blocks disruption of blood brain barrier in a rabbit model of Alzheimer's disease-1

His effect was blocked by caffeine at the dose of 3 mg/day. Caffeine alone had no effect on extravasation of fibrinogen in normal rabbit brain. Representative images taken from 2 rabbits in each group with 6 sections from each animal are shown. Bar = 50 μm. (B) Cholesterol-enriched diet significantly increased accumulation of fibrinogen in olfactory bulb and this effect was blocked by caffeine at the dose of 3 mg/day. (C) Neither cholesterol-enriched diet nor caffeine changed significantly plasma levels of fibrinogen. n = 4, *p < 0.05.<p><b>Copyright information:</b></p><p>Taken from "Caffeine blocks disruption of blood brain barrier in a rabbit model of Alzheimer's disease"</p><p>http://www.jneuroinflammation.com/content/5/1/12</p><p>Journal of Neuroinflammation 2008;5():12-12.</p><p>Published online 3 Apr 2008</p><p>PMCID:PMC2330033.</p><p></p

Caffeine blocks disruption of blood brain barrier in a rabbit model of Alzheimer's disease-0

Ect was blocked by caffeine at the dose of 3 mg/day. Caffeine alone had no effect on extravasation of IgG in normal rabbit brain. Representative images taken from 2 rabbits in each group with 6 sections from each animal are shown. Bar = 50 μm. (B) Cholesterol-enriched diet significantly increased accumulation of IgG in olfactory bulb and this effect was blocked by caffeine at the dose of 3 mg/day. (C) Neither cholesterol-enriched diet nor caffeine changed significantly plasma levels of IgG. n = 4, *p < 0.05.<p><b>Copyright information:</b></p><p>Taken from "Caffeine blocks disruption of blood brain barrier in a rabbit model of Alzheimer's disease"</p><p>http://www.jneuroinflammation.com/content/5/1/12</p><p>Journal of Neuroinflammation 2008;5():12-12.</p><p>Published online 3 Apr 2008</p><p>PMCID:PMC2330033.</p><p></p

Caffeine blocks disruption of blood brain barrier in a rabbit model of Alzheimer's disease-8

Ect was blocked by caffeine at the dose of 3 mg/day. Caffeine alone had no effect on extravasation of IgG in normal rabbit brain. Representative images taken from 2 rabbits in each group with 6 sections from each animal are shown. Bar = 50 μm. (B) Cholesterol-enriched diet significantly increased accumulation of IgG in olfactory bulb and this effect was blocked by caffeine at the dose of 3 mg/day. (C) Neither cholesterol-enriched diet nor caffeine changed significantly plasma levels of IgG. n = 4, *p < 0.05.<p><b>Copyright information:</b></p><p>Taken from "Caffeine blocks disruption of blood brain barrier in a rabbit model of Alzheimer's disease"</p><p>http://www.jneuroinflammation.com/content/5/1/12</p><p>Journal of Neuroinflammation 2008;5():12-12.</p><p>Published online 3 Apr 2008</p><p>PMCID:PMC2330033.</p><p></p

Caffeine blocks disruption of blood brain barrier in a rabbit model of Alzheimer's disease-3

Uorescent images were taken for measures of the expression of occludin and ZO-1. (B) Decreased occludin immunostaining was observed in olfactory bulb from cholesterol-fed rabbits and this effect was blocked by caffeine. Caffeine alone had no effect on occludin immunostaining in normal rabbit brain. Representative images taken from 2 rabbits in each group with 6 sections from each animal are shown. Bar = 100 μm. (C) Quantitative data from B shows that high cholesterol diet significantly decreased occludin immunopositive staining in olfactory bulb, and this effect is blocked by caffeine. (D) Cholesterol-enriched diet decreased significantly protein levels of occludin, and these effects were blocked by caffeine at the dose of 3 mg/day. Caffeine alone did not significantly change protein levels of occludin in normal rabbit olfactory bulb (n = 4, *p < 0.05). (E) Decreased ZO-1 immunostaining was observed in olfactory bulb from cholesterol-fed rabbits and this effect was blocked by caffeine. Caffeine alone had no effect on ZO-1 immunostaining in normal rabbit brain. Representative images taken from 2 rabbits in each group with 6 sections from each animal are shown. Bar = 100 μm. (F) Quantitative data from E shows that high cholesterol diet significantly decreased ZO-1 immunopositive staining in olfactory bulb, and this effect is blocked by caffeine. (G) Cholesterol-enriched diet decreased significantly protein levels of ZO-1, and these effects were blocked by caffeine at the dose of 3 mg/day. Caffeine alone did not significantly change protein levels of ZO-1 in normal rabbit olfactory bulb. n = 4, *p < 0.05.<p><b>Copyright information:</b></p><p>Taken from "Caffeine blocks disruption of blood brain barrier in a rabbit model of Alzheimer's disease"</p><p>http://www.jneuroinflammation.com/content/5/1/12</p><p>Journal of Neuroinflammation 2008;5():12-12.</p><p>Published online 3 Apr 2008</p><p>PMCID:PMC2330033.</p><p></p

Caffeine blocks disruption of blood brain barrier in a rabbit model of Alzheimer's disease-7

Hed diet markedly increased the numbers of microglia (green, arrow heads) and IgG extravasation (red). These effects were blocked by caffeine at the dose of 3 mg/day for 12 weeks. Representative images taken from 2 rabbits in each group with 6 sections from each animal are shown. Bar = 50 μm. (B) In a magnified view of the white boxes as shown in panel A, increased microglia (arrow heads) are co-distributed with perivascular immunopositive IgG staining (arrows) in olfactory bulb from cholesterol-fed rabbits. Bar = 20 μm.<p><b>Copyright information:</b></p><p>Taken from "Caffeine blocks disruption of blood brain barrier in a rabbit model of Alzheimer's disease"</p><p>http://www.jneuroinflammation.com/content/5/1/12</p><p>Journal of Neuroinflammation 2008;5():12-12.</p><p>Published online 3 Apr 2008</p><p>PMCID:PMC2330033.</p><p></p

Caffeine blocks disruption of blood brain barrier in a rabbit model of Alzheimer's disease-6

Und the sites where IgG extravasation (red) was present. These effects were blocked by caffeine at the dose of 3 mg/day. Representative images taken from 2 rabbits in each group with 6 sections from each animal are shown. Bar = 20 μm.<p><b>Copyright information:</b></p><p>Taken from "Caffeine blocks disruption of blood brain barrier in a rabbit model of Alzheimer's disease"</p><p>http://www.jneuroinflammation.com/content/5/1/12</p><p>Journal of Neuroinflammation 2008;5():12-12.</p><p>Published online 3 Apr 2008</p><p>PMCID:PMC2330033.</p><p></p