19 research outputs found

    第774回 千葉医学会例会・第二内科例会 54.

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    InterProScan results. InterProScan [34] queries sequences against 16 member databases enabling protein classification by family as well as by conserved domains; the number of annotations and the percent of sequences receiving annotations varied across the different databases. (XLSX 32 kb

    Verification of lignocellulytic activity of <i>A. glabripennis</i> derived <i>F. solani</i> solid wood culture extracts through in vitro assays.

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    <p>Activities of cellulases, xylanases, and lignin peroxidases, Mn-dependent and independent peroxidases, as well as laccase were measured for <i>F. solani</i> extracts with each assay containing 50 µg of protein.</p>*<p>1 unit of activity = amount of enzyme that releases 1 µmol of reducing sugar per minute for reducing sugar assays and amount of enzyme that oxidizes 1 µmol substrate per min for peroxidase assays.</p

    Other cell wall degrading proteins from MudPIT analysis.

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    <p>Other cell wall degrading proteins from MudPIT analysis.</p

    Proteinases and nitrogen recycling proteins identified from MudPIT data.

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    <p>Proteinases and nitrogen recycling proteins identified from MudPIT data.</p

    Enrichment of GO Molecular Function terms in proteomic analysis.

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    <p>Bar graph represents the ratio of % composition of term in proteomic data vs. % composition in the genome annotation. Values over 1 (dotted line) are overrepresented in the proteomic data. Red line illustrates the relative abundance of the GO term in the proteomic data.</p

    Heme staining and zymogram analysis of <i>A. glabripennis</i> derived <i>F. solani</i> solid wood culture extract.

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    <p>Twenty µg of fungal extract were loaded into each lane. Protein standard is on the left, with band sizes labeled (kDa). Lane A is a colloidal blue stained lane, and lane B is the corresponding heme stain/CMC/or xylan zymogram lane. For heme stain, a single band is present at approximately 70 kDa. For CMC zymogram six major zones of clearing are present at approximately 55, 32, 30, 27, 23 and 20 kDa and can be matched to protein bands on the colloidal blue stained lane. For birch wood xylan zymogram, many zones of clearing are present on the gel with major spots between 30 and 50 kDa. These correspond to a broad range of bands on the colloidal blue stained lane.</p

    Glycoside hydrolase (GH) families detected in MudPIT analysis.

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    <p>Glycoside hydrolase (GH) families detected in MudPIT analysis.</p

    Proteins associated with lignin metabolism from MudPIT analysis.

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    <p>Proteins associated with lignin metabolism from MudPIT analysis.</p

    Most Abundant InterPro IDs Identified in MudPIT analysis.

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    <p>Most Abundant InterPro IDs Identified in MudPIT analysis.</p
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