Construction of 1‑Naphthols via Benzannulation Based on the Reaction of Aryl <i>tert</i>-Butyl Ynol Ethers with Ynamides or Ynol Ethers

A new version of benzannulation featuring the use of aromatic <i>tert</i>-butyl ynol ethers as the convenient precursors for arylketenes has been developed. Both ynamides and ynol ethers undergo this reaction smoothly, giving 3-amino and 3-alkoxy 1-naphthols in good to excellent yields under the heated reaction conditions. The high efficiency, excellent regioselectivity, good functional group compatibility, and broad substrate scope render this reaction particularly valuable for organic synthesis

Silver-Catalyzed Regio- and Stereoselective Addition of Carboxylic Acids to Ynol Ethers

A silver-catalyzed <i>trans</i> addition of carboxylic acids to ynol ethers is described. The reaction has a broad scope with respect to carboxylic acids and ynol ethers, delivering (<i>Z</i>)-α-alkoxy enol esters in good yields with excellent regio- and stereoselectivity. Meaningfully, the Ni-catalyzed selective coupling of alkenyl C–OPiv bonds of (<i>Z</i>)-α-alkoxy enol esters with boronic acids enables a convenient route to the access of (<i>E</i>)-enol ethers. As such, the two-step procedure, consisted of a hydrocarboxylation and a subsequent Suzuki–Miyaura coupling, offers a formal <i>trans</i> hydroarylation of ynol ethers, thus providing a good complementary method to our previous report

A method for LAMOST cofocus maintenance based on eddy current edge sensor

The Large Sky Area Multi-Object Spectroscopic Telescope (LAMOST) is currently the largest field of view in use internationally. Its primary mirror Mb is spliced by 37 hexagonal segments with a diagonal length of 1.1 meters. The current cofocus maintenance strategy using Shack-Hartmann wavefront sensor (SHWFS) would interrupt the observation mission, a kind of eddy current edge sensor with high-precision and accuracy is proposed in this article to achieve the closed-loop cofocus control. Through the structural design, interaction matrix deduction and installation parameters analysis, it can be concluded that the sensor structure with 1.35° inclination compensation for dihedral angle between the segments on Mb is feasible. The minimum cofocus maintenance system (MCMS) is established by separating mirror No.27, No.32 and No.33 from Mb. The result of cofocus maintenance experiment indicates that the closed-loop control system is still effective within less than 1.5℃ environmental temperature changes after 13 hours, which is much longer than the maintenance time of SHWFS, and continuous observation without interrupt is possible during one observation night

Presentation_1_Halolysin SptA, a Serine Protease, Contributes to Growth-Phase Transition of Haloarchaeon Natrinema sp. J7-2, and Its Expression Involves Cooperative Action of Multiple Cis-Regulatory Elements.pdf

<p>Many haloarchaea produce extracellular subtilisin-like proteases (halolysins) during late log phase; however, the physiological function and regulatory mechanism of growth phase-dependent production of halolysins are unknown. Halolysin SptA, the major extracellular protease of Natrinema sp. J7-2, is capable of intracellular self-activation to affect haloarchaeal growth. Here, we report that deletion of sptA leads to loss of extracellular and intracellular protease activities against azocasein and/or suc-AAPF-pNA, as well as a change in growth-phase transition of the haloarchaeon. Our results suggest that SptA is important for strain J7-2 to enter the stationary and death phases. Deletion and mutational analyses of the 5′-flanking region of sptA revealed two partially overlapping, semi-palindromic sequences upstream of the TATA box act as positive and negative cis-regulatory elements, respectively, to mediate sptA expression in late log phase. Additionally, a negative cis-regulatory element covering WW motif and a distant enhancer contribute to the modulation of sptA expression. Our results demonstrate that SptA functions both extracellularly and intracellularly, and that sptA expression relies on the cooperative action of multiple cis-regulatory elements, allowing SptA to exert its function properly at different growth stages in strain J7-2.</p

Palladium-Catalyzed Hydroarylation, Hydroalkenylation, and Hydrobenzylation of Ynol Ethers with Organohalides: A Regio- and Stereoselective Entry to α,β- and β,β-Disubstituted Alkenyl Ethers

A Pd-catalyzed reductive addition of organohalides, including aryl, alkenyl, and benzyl halides, to ynol ethers has been realized in the presence of 2-propanol, giving α,β- and β,β-disubstituted olefinic ethers in satisfactory yields with excellent regio- and stereoselectivity. It represents the first highly regio- and stereoselective hydroarylation, hydroalkenylation, and hydrobenzylation of ynol ethers

Additional file 2: Figure S1.

Nucleotide sequence and deduced amino acid sequence of BpMYC4 from Betula platyphylla. (DOCX 287 kb

Additional file 3: Figure S2.

Nucleotide sequence and deduced amino acid sequence of BpbHLH9 from Betula platyphylla. (DOCX 193 kb

Additional file 5: Figures S5, S6 and S7.

Identification and acquisition of BpbHLH9 transgenic seedlings. (DOCX 730 kb

Additional file 1: Table S1. of Cloning and expression of BpMYC4 and BpbHLH9 genes and the role of BpbHLH9 in triterpenoid synthesis in birch

List of primers used in the study. (DOCX 16 kb

AFM images of the outer and inner surface of uninfected and infected erythrocytes.

<p>(a) Intact uninfected erythrocyte; (b) cytoplasmic-surface-exposed uninfected erythrocyte membrane (one layer); (c) Intact trophozoite stage infected erythrocytes; (d) cytoplasmic-surface-exposed trophozoite stage infected erythrocyte membrane (one layer); (e-f) Plot view of high magnification of (e) uninfected erythrocyte membrane outer surface, (f) uninfected erythrocyte membrane inner surface, (g) infected erythrocyte membrane outer surface and (h) infected erythrocyte membrane inner surface. Knobs were indicated by black arrows. Bars: 4 µm (a-d); 500 nm (e-h).</p