MMP1 protein levels in the cell culture medium.

<p><b>A</b>: AGS cells. <b>B</b>: Hs746T cells. </p

Molecular changes related to migration.

<p>Heatmap for genes differentially expressed after MPA treatment for 0h, 12h, 24h, 48h and 72h. Each sample is represented in a column and each gene is represented in a row. Increased expression is indicated as red and decreased expression is indicated as green. Representative genes are shown on the right panel and gene clusters are indicated on the left. </p

Confirmation of gene expression differences by real-time RT-PCR.

<p>Box blots are shown for each gene. Relative expression levels are shown in log 2 scale. FC (fold change) for each time point (12h, 24h and 48h) is compared to untreated controls (0h) with respective p-values.</p

A network illustrating the connectedness of the genes (>2-fold differences) involved in cell migration.

<p>A network illustrating the connectedness of the genes (>2-fold differences) involved in cell migration.</p

MPA modulates the migration and invasion abilities of AGS cells but not Hs746T cells.

<p><b>A</b>: Images of migrating cells after treatment with vehicle (DMSO) or different concentration of MPA for 24h. The mean relative migrating cells are shown at the bottom. <b>B</b>: Images of invading cells after treatment with vehicle (DMSO) or different concentration of MPA for 24h. The mean relative invading cells are shown at the bottom. *p < 0.05 compared to DMSO control.</p

STAT5 phosphorylation in Stat5b transgenic mice.

<p>(A) Representative Western blotting analysis for phosphorylated and total STAT5 in F1 and NOD Stat5b transgenic lines. These mice did not have detectable signs of lymphoma based on physical examination and FACS analysis of T cell phenotypes. Protein extracts from thymus were used for the experiment. (B) Representative Western blotting analysis of phosphorylated STAT5 with thymus protein extracts from NOD.Stat5b transgenic mice with lymphoma. (C) STAT5 phosphorylation status in different cell types. Thymocytes and splenocytes from NOD Stat5b^Tg mice were analyzed by intracellular staining for phosphorylated STAT5 with an anti–pTyr694-STAT5 antibody. (D) FACS analysis showing progressive increase of STAT5 phosphorylation in thymocytes of NOD.Stat5b^Tg mice. Data for thymus are shown here for 6, 12 and 16 week old NOD.Stat5b^Tg mice (all without tumor). Representative data are shown from 1 of 3 similar experiments.</p

The role of IL-10 and IFN-γ in the generation and function of the CD8NKT-like cells

Cytokine levels in the cell culture media. Cultured CD8NKT-like cells and freshly isolated CD4CD25Treg cells were stimulated with anti-CD3 (1.5 μg/ml) and splenic APCs. At 72 h of culturing, the culture supernatant was saved and used for measuring IL-10, IL-4 and IFN-γ using ELISA. Results are representative of two independent experiments. Suppression activity of CD8NKT-like cells cultured from IFN-γand IL-10mice. CD8NKT-like cells (Tr) cultured from knockout mice and wild-type B6 (WT) mice were co-cultured with naïve CD4CD25responder T cells (Tn) at different Tr/Tn ratios in the presence of splenic APCs and anti-CD3. The cultures were pulsed with 1 μCi/well of [H]thymidine at 72 h and proliferation (cpm) was measured by [H]thymidine incorporation in the last 16 h. Results are expressed as the mean of triplicate cultures. ANOVA -values are <p><b>Copyright information:</b></p><p>Taken from "The IL-10 and IFN-γ pathways are essential to the potent immunosuppressive activity of cultured CD8NKT-like cells"</p><p>http://genomebiology.com/2008/9/7/R119</p><p>Genome Biology 2008;9(7):R119-R119.</p><p>Published online 29 Jul 2008</p><p>PMCID:PMC2530876.</p><p></p

Total cell numbers in the thymus, bone marrow and spleen of NOD.Stat5b transgenic mice and littermate controls.

<p>Cell numbers (10⁶) are the averages for NOD.Stat5b^Tg transgenic mice and their non-transgenic littermate control mice (LMC). Mice at 6 weeks (6 wk), 10 weeks (10 wk) and 16 weeks (16 wk) of age were examined. Student t tests were used to assess statistical significance.</p>#<p>, <i>P</i>>0.05;</p>*<p>, <i>P</i><0.05;</p>**<p>, <i>P</i><0.01 compared with LMC.</p

Heat map for genes differentially expressed among the four groups of T cells

Only those genes with a FDR (q) ≤0.01 and fold change ≥5 are included in this map. Data for each gene are standardized separately before being plotted, as is standard in drawing heat maps, so that all genes have a similar scale and the relative differences for all genes can be visualized on a single plot.<p><b>Copyright information:</b></p><p>Taken from "The IL-10 and IFN-γ pathways are essential to the potent immunosuppressive activity of cultured CD8NKT-like cells"</p><p>http://genomebiology.com/2008/9/7/R119</p><p>Genome Biology 2008;9(7):R119-R119.</p><p>Published online 29 Jul 2008</p><p>PMCID:PMC2530876.</p><p></p

Phenotypes of CD8⁺ lymphomas and T cell development in NOD.Stat5b^Tg mice.

<p>(A) Three different types of lymphoma cells are illustrated. The vast majority of lymphomas (57/60) are similar to the Onco 2 mouse, e.g., with CD4⁺CD8⁺ double positive and CD8⁺ single positive thymocytes. Two mice with lymphomas had predominantly CD8⁺ single-positive thymocytes (Onco 18) and one mouse had predominantly CD4⁺CD8⁺ double-positive thymocytes (Onco 29). (B) Phenotypes of metastatic tumors. Tumor phenotypes are identical in different lymphoid organs including spleen (SP), thymus (Thy) and cervical lymph node (CN) (a, b, c). Tumor cells (5×10⁶) from thymus of the mouse as shown in b were subcutaneously injected at the back of regular NOD mice. Tumor formation was observed at the site of injection 10–20 days later and tumor cells also migrate to other lymphoid organs including spleen (d), thymus (e) and cervical node (f). Tumor phenotypes are identical at the injection site (g, dorsal) and other lymphoid organs. (C) CD25 expression in thymus of NOD.Stat5b^Tg mice and littermate controls at 6 weeks and lymphoma population in cervical node. (D) T cell phenotypes in the thymus (Thy) and spleen (Sp) of NOD.Stat5b^Tg mice and littermate controls at 6 weeks (6 w) and 12 weeks (12 w) of age. (E) T cell phenotypes in the thymus (Thy) and spleen (Sp) of NOD/B6 F1.Stat5b^Tg mice and littermate controls at 6 weeks (6 w) and 12 weeks (12 w) of age. (F) CD44 and CD122 expression of CD8⁺ T cells from spleens and thymi of NOD.Stat5b^Tg mice (16 weeks of age). Representative data are shown from 1 of 4 similar experiments.</p