Cytokine levels in the cell culture media. Cultured CD8NKT-like cells and freshly isolated CD4CD25Treg cells were stimulated with anti-CD3 (1.5 μg/ml) and splenic APCs. At 72 h of culturing, the culture supernatant was saved and used for measuring IL-10, IL-4 and IFN-γ using ELISA. Results are representative of two independent experiments. Suppression activity of CD8NKT-like cells cultured from IFN-γand IL-10mice. CD8NKT-like cells (Tr) cultured from knockout mice and wild-type B6 (WT) mice were co-cultured with naïve CD4CD25responder T cells (Tn) at different Tr/Tn ratios in the presence of splenic APCs and anti-CD3. The cultures were pulsed with 1 μCi/well of [H]thymidine at 72 h and proliferation (cpm) was measured by [H]thymidine incorporation in the last 16 h. Results are expressed as the mean of triplicate cultures. ANOVA -values are <p><b>Copyright information:</b></p><p>Taken from "The IL-10 and IFN-γ pathways are essential to the potent immunosuppressive activity of cultured CD8NKT-like cells"</p><p>http://genomebiology.com/2008/9/7/R119</p><p>Genome Biology 2008;9(7):R119-R119.</p><p>Published online 29 Jul 2008</p><p>PMCID:PMC2530876.</p><p></p
