Individual Professional Practice in the Company

Import 23/08/2017Cílem této bakalářské práce je popsat absolvování odborné praxe ve firmě HS Interactive s.r.o. Praxe byla zaměřena na vývoj mobilní aplikace pro operační systém Android. Aplikace je mobilním klientem pro sociální síť MatchToMe. V úvodu popisuji důvody, které vedly k výběru odborné praxe. Dále se věnuji úkolům, které mi byly zadány s jejich implementací a postupem řešení problémů, které se objevily při vývoji. Závěr práce je věnován zhodnocení získaných zkušeností a dosažených výsledků.Purpose of this bachelor thesis is to describe a professional practice in company HS Interactive s.r.o. Practice was focused on the development of mobile application for the operating system Android. The application is a mobile client for social network MatchToMe. In the introduction I describe reasons that led to the selection of professional practice. Then I describe tasks that I have been awarded with their implementations and process of solution issues that have emerged during development. The conclusion of thesis is dedicated to the evaluation of the experience gained and the results achieved.440 - Katedra telekomunikační technikyvýborn

Canonical structural organization of the IGS regions in (A) Yunnan82-114 (<i>S</i>. <i>spontaneum</i>), (B) 51NG3 (<i>S</i>. <i>robustum</i>) and (C) Luohanzhe (<i>S</i>. <i>officinarum</i>).

<p>IGS: intergenic spacer; TTS: transcription termination site; NTS: non-transcribed spacer; TIS: transcription initiation site; ETS: external transcribed sequence; SR: sub-repeat. CpG island: cytosine-guanine island.</p

Comparison of putative TIS of different plants.

<p>The respective accession numbers: Yunnan82-114 (<i>S</i>. <i>spontaneum</i>) KX254601; 51NG3 (<i>S</i>. <i>robustum</i>) KX254591; Luohanzhe (<i>S</i>. <i>officinarum</i>) KX254598; <i>O</i>. <i>sativa</i> X54194; <i>Z</i>. <i>mays</i> X03990; <i>S</i>. <i>cereale</i> M37231; <i>T</i>. <i>aestivum</i> AJ315040; <i>M</i>. <i>sinensis</i> AJ238126; <i>C</i>. <i>sativus</i> X51542; <i>C</i>. <i>maxima</i> M28700; <i>C</i>. <i>pepo</i> X55960; <i>V</i>. <i>radiata</i> X17211; <i>V</i>. <i>faba</i> X16615; <i>S</i>. <i>mexicana</i> AJ489509; <i>B</i>. <i>juncea</i> X73032; <i>B</i>. <i>rapa</i> S78172; <i>B</i>. <i>oleracea</i> X60324; <i>A</i>. <i>thaliana</i> X52631; <i>R</i>. <i>sativus</i> Z11677; <i>N</i>. <i>tabacum</i> Y08422; <i>S</i>. <i>lycopersicum</i> AY366528; <i>S</i>. <i>tuberosum</i> AF464863; <i>C</i>. <i>annuum</i> HM352915; <i>O</i>. <i>europaea</i> AJ865373; <i>P</i>. <i>granatum</i> JX121275; <i>F</i>. <i>sylvatica</i> KC700362; <i>Q</i>. <i>suber</i> AY428812; <i>Q</i>. <i>petraea</i> EU555524; <i>Q</i>. <i>robur</i> EF208969.</p

Methylation sites in the IGS sequence.

<p>Methylation sites in the IGS sequence.</p

The 15 genotypes of the genus <i>Saccharum</i> used in this study.

<p>The 15 genotypes of the genus <i>Saccharum</i> used in this study.</p

Physical mapping of IGS and pTa71 probe in Yunnan82-114 (<i>S</i>. <i>spontaneum</i>), 51NG3 (<i>S</i>. <i>robustum</i>) and Luohanzhe (<i>S</i>. <i>officinarum</i>).

<p>FISH with IGS (red- B, F, J), and wheat rDNA pTa71 probe (green- A, E, I) in meristematic root tip metaphase chromosomes of Yunnan82-114 (A-D), 51NG3 (E-H), and Luohanzhe (I-L). DNA is counterstained with DAPI (blue—C, G, K). The fourth column shows the merged images of both signals of IGS and wheat rDNA pTa71 probe and DAPI-stained chromosomes (D, H, L). Arrows indicate minor loci NORs (A, B, D; E, F, H; I, J, L). Scale bars = 5 μm.</p

Dot matrix plot of IGS spacers in (A) Yunnan82-114, (B) 51NG3 and (C) Luohanzhe.

<p>IGS spacer was performed using a k-tuple of 8 with 100% identity.</p

Comparative genetic analysis of the 45S rDNA intergenic spacers from three <i>Saccharum</i> species

<div><p>The 45S ribosomal DNA (rDNA) units are separated by an intergenic spacer (IGS) containing the signals for transcription and processing of rRNAs. For the first time, we sequenced and analyzed the entire IGS region from three original species within the genus <i>Saccharum</i>, including <i>S</i>. <i>spontaneum</i>, <i>S</i>. <i>robustum</i>, and <i>S</i>. <i>officinarum</i> in this study. We have compared the IGS organization within three original species of the genus <i>Saccharum</i>. The IGS of these three original species showed similar overall organizations comprised of putative functional elements needed for rRNA gene activity as well as a non-transcribed spacer (NTS), a promoter region, and an external transcribed spacer (ETS). The variability in length of the IGS sequences was assessed at the individual, intraspecies, and interspecies levels of the genus <i>Saccharum</i>, including <i>S</i>. <i>spontaneum</i>, <i>S</i>. <i>robustum</i>, and <i>S</i>. <i>officinarum</i>. The ETS had greater similarity than the NTS across species, but nevertheless exhibited variation in length. Within the IGS of the <i>Saccharum</i> species, base substitutions and copy number variation of sub-repeat were causes of the divergence in IGS sequences. We also identified a significant number of methylation sites. Furthermore, fluorescent <i>in situ</i> hybridization (FISH) co-localization of IGS and pTa71 probes was detected on all representative species of the genus <i>Saccharum</i> tested. Taken together, the results of this study provide a better insight into the structure and organization of the IGS in the genus <i>Saccharum</i>.</p></div

Phylogenetic relationships among 15 accessions of the genus <i>Saccharum</i>.

<p>Bootstrap values from 1000 tests are indicated at the nodes. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The ML and NJ tree of the same data is topologically identical.</p

The intergeneric BC₂ and BC₃ progeny between <i>Saccharum</i> spp. and <i>E</i>. <i>arundinaceus</i>.

<p><b>Note</b>: “YCE” series are the progeny of <i>E</i>. <i>arundinaceus</i>, the other plant materials are the commercial cultivars containing germplasm from <i>Saccharum</i> spp. without contribution from <i>E</i>. <i>arundinaceus</i>.</p><p>The intergeneric BC₂ and BC₃ progeny between <i>Saccharum</i> spp. and <i>E</i>. <i>arundinaceus</i>.</p