Restrikční endonukleázy a genetické testy

This bachelor thesis Restriction endonucleases and genetic tests engages in the issue of restriction enzymes. In the literary review I describe the character of restriction endonucleases, their classification and mechanism of operation. The thesis furthermore touches a topic concerning the effect of the restriction reaction conditions and discusses techniques of utilization of these enzymes, where for example DNA cloning, analysis of variability or genome mapping belong. The practical part of the thesis includes some work with a software for an automatic search for restriction sites and for restriction DNA evaluation

Additional file 1 of The succession pattern of soil microbial communities and its relationship with tobacco bacterial wilt

Figure S1. Rarefaction curves of 16r RNA gene sequencing data. Figure S2. Composition and structure of soil microbial communities in each group. Figure S3. Phylogenetic molecular ecological networks (pMEN) of microbial communities in each group, and number of nodes and links of each pMEN. Table S1. Soil properties. Table S2(a). Correlation of microbial populations in abundance between two periods at the phylum level. (b). Correlation of microbial populations in abundance between two periods at the genus level. Table S3. Topological properties of the empirical pMENs of microbial communities in eight groups. Table S4(a). Correlation between abundance of microbial populations and tobacco morbidity at the phylum level. (b). Correlation between abundance of microbial populations and tobacco morbidity at the genus level. Table S5(a). Mantel test of sequencing data with environmental attributes at the phylum level. (b). Mantel test of sequencing data with environmental attributes at the genus level. (DOCX 1100 kb

Additional file 1: of Comparison of microbial taxonomic and functional shift pattern along contamination gradient

Table S1. Sediment properties and ANOVA analysis among three groups. Table S2. Shannon diversity and Pielou evenness of samples based on sequencing data and Geochip hybridization data respectively. Table S3. Correlation between microbial composition and function. Table S4. (a) Mantel test of sequencing data with environmental attributes at the genus level; (b) Mantel test of GeoChip hybridization data with environmental attributes. (DOCX 41 kb