Additional file 2: Figure S2. of Caveolin-1 regulates the expression of tight junction proteins during hyperoxia-induced pulmonary epithelial barrier breakdown

Effects of transfection concentration and transfection time of Cav-1 cDNA on mRNA and protein expression of Cav-1 in alveolar cell monolayers. At 48 h after transfection of 1 μg/mL Cav-1 cDNA, significant increases in caveolin-1 mRNA (A, B, E, F) and protein (C, D, G, H) were readily apparent. mRNA and protein expressions were determined by RT-PCR and Western blot analysis, respectively. β-actin was used as an internal control. Values are represented as means ± SD, ## P < 0.01 for comparison between the normoxia and CAV1 cDNA-transfected group, ** P < 0.01 for comparison between different concentration transfected groups or different time ransfected groups. (TIF 1801 kb

Additional file 3: Figure S3. of Caveolin-1 regulates the expression of tight junction proteins during hyperoxia-induced pulmonary epithelial barrier breakdown

Efficacy assessment of Cav-1-siRNA transfection and pCMV6-CAV1 transfection. At 60–70 % confluence, cells exposed to normoxia were transfected with 10 nm of a control siRNA (Fluorescein Conjugate) (Cat.#6201;Cell Signaling Danvers MA) for 72 h to assess transfection efficiency for Cav-1-siRNA, while cells exposed to normoxia or hyperoxia were transfected with 1 μg/mL of pCMV6-AC-GFP (Cat.#PS100010; OriGene Technologies) for 48 h to assess transfection efficiency for pCMV6-CAV1. Images were obtained using a confocal laser scanning microscope at 100× magnification. Green represents transfected cells. The areas of transfected cells and all the cells in the view were measured by image pro plus software, and the ratio represents transfection efficiency. The transfection efficiency of control siRNA was (88.29 ± 8.25)% (A, B). The transfection efficiency of pCMV6-AC-GFP under normoxic conditions was (92.81 ± 4.16)% (C, D). The transfection efficiency of pCMV6-AC-GFP under hyperoxic conditions was (90.42 ± 6.83)% (E, F). (TIF 10078 kb

Additional file 1: of Salvianolic acid B induced upregulation of miR-30a protects cardiac myocytes from ischemia/reperfusion injury

Availability of supporting data. (XLSX 14 kb

Additional file 2: Figure S3. of Ginsenoside Rg1 protects starving H9c2 cells by dissociation of Bcl-2-Beclin1 complex

The effect of CQ on different time points. The LC3 level significantly increased at 90 min (*p < 0.01). Figure S4. The effect of 3-MA on different time points. The LC3 level significantly decreased at 12 h (*p < 0.05) and 24 h (*p < 0.05). Figure S5. The effect of Rap on different time points. The LC3 level significantly increased at 12 h (**p < 0.01) and 24 h (*p < 0.05). (XLSX 33 kb

Additional file 1: Figure S1. of Ginsenoside Rg1 protects starving H9c2 cells by dissociation of Bcl-2-Beclin1 complex

Cell viability of H9c2 cells. Cell viability significantly decreased in a time-dependent manner between 30 and 240 min when compared with the control (0 min). Values are expressed as the mean ± SD, n = 3. ** p < 0.01, starvation model group verse control group. Figure S2. Effects of ginsenoside Rg1 on starvation-induced apoptosis in H9c2 cells using flow cytometric analysis. (PDF 346 kb

Top ten of Enriched Gene Ontology(GO) terms associated with the differentially expressed genes.

<p>Top ten of Enriched Gene Ontology(GO) terms associated with the differentially expressed genes.</p

Top ten up- regulated and down-regulated genes in myocardial ischemia.

<p>Top ten up- regulated and down-regulated genes in myocardial ischemia.</p

Statics of reads mapped to reference genes.

<p>Statics of reads mapped to reference genes.</p

Statistics of reads mapped to reference genome.

<p>Statistics of reads mapped to reference genome.</p

Top ten of pathways associated with the differentially expressed genes.

<p>Top ten of pathways associated with the differentially expressed genes.</p