Additional file 3: Figure S3. of Caveolin-1 regulates the expression of tight junction proteins during hyperoxia-induced pulmonary epithelial barrier breakdown
Efficacy assessment of Cav-1-siRNA transfection and pCMV6-CAV1 transfection. At 60–70 % confluence, cells exposed to normoxia were transfected with 10 nm of a control siRNA (Fluorescein Conjugate) (Cat.#6201;Cell Signaling Danvers MA) for 72 h to assess transfection efficiency for Cav-1-siRNA, while cells exposed to normoxia or hyperoxia were transfected with 1 μg/mL of pCMV6-AC-GFP (Cat.#PS100010; OriGene Technologies) for 48 h to assess transfection efficiency for pCMV6-CAV1. Images were obtained using a confocal laser scanning microscope at 100× magnification. Green represents transfected cells. The areas of transfected cells and all the cells in the view were measured by image pro plus software, and the ratio represents transfection efficiency. The transfection efficiency of control siRNA was (88.29 ± 8.25)% (A, B). The transfection efficiency of pCMV6-AC-GFP under normoxic conditions was (92.81 ± 4.16)% (C, D). The transfection efficiency of pCMV6-AC-GFP under hyperoxic conditions was (90.42 ± 6.83)% (E, F). (TIF 10078 kb
