24 research outputs found
The role of the triangle singularity in production in the and processes
We have investigated the cross section for the and reactions paying attention to a
mechanism that develops a triangle singularity. The triangle diagram is
realized by the decay of a to and the decay into , and the finally merges into . The mechanism is
expected to produce a peak around MeV in the invariant
mass. We found that a clear peak appears around MeV in the
invariant mass which is about MeV lower than the
expectation, and that is due to the resonance peak of a resonance which
plays a crucial role in the production. The mechanism studied
produces the peak of the around or below 1400 MeV, as is seen
in the HADES experiment.Comment: 12 pages, 9 figure
Coccinellidae combined dataset: consensus tree
Consensus of 50K trees from Bayesian analysis, excluding 3k burnin
Coccinellidae nucleic acid and morphological data matrix
Partitioned nexus file containing molecular and morphological data, used for Bayesian inference of coccinellid phylogeny. Includes the loci COI, COII, and 28S
Split-Cre Complementation Restores Combination Activity on Transgene Excision in Hair Roots of Transgenic Tobacco
<div><p>The Cre/loxP system is increasingly exploited for genetic manipulation of DNA <i>in vitro</i> and <i>in vivo</i>. It was previously reported that inactive ‘‘split-Cre’’ fragments could restore Cre activity in transgenic mice when overlapping co-expression was controlled by two different promoters. In this study, we analyzed recombination activities of split-Cre proteins, and found that no recombinase activity was detected in the <i>in vitro</i> recombination reaction in which only the N-terminal domain (NCre) of split-Cre protein was expressed, whereas recombination activity was obtained when the C-terminal (CCre) or both NCre and CCre fragments were supplied. We have also determined the recombination efficiency of split-Cre proteins which were co-expressed in hair roots of transgenic tobacco. No Cre recombination event was observed in hair roots of transgenic tobacco when the NCre or CCre genes were expressed alone. In contrast, an efficient recombination event was found in transgenic hairy roots co-expressing both inactive split-Cre genes. Moreover, the restored recombination efficiency of split-Cre proteins fused with the nuclear localization sequence (NLS) was higher than that of intact Cre in transgenic lines. Thus, DNA recombination mediated by split-Cre proteins provides an alternative method for spatial and temporal regulation of gene expression in transgenic plants.</p></div
MOESM3 of Engineered global regulator H-NS improves the acid tolerance of E. coli
Additional file 3: Table S2. Genes differently expressed at transcriptional level between strain 3-36 and wild type strain MG
DNA oligo sequences utilizes in this report.
<p>DNA oligo sequences utilizes in this report.</p
Digram of the split-Cre model and <i>in vitro</i> recombination of Split-Cre protein.
<p><b>A: Digram of the split-Cre model.</b> The intact Cre was designed to be split at the 60th amino acid residue. Two molecules of split-Cre were named NCre and CCre respectively. <b>B: Structure of the substrate catalyzed by purified protein. C: Recombination assay of Split-Cre protein </b><b><i>in vitro</i></b><b>.</b> M: DL5000 Marker; Plasmid: 2µl plasmid (90 ng/µl) of pLoxp-Ic-CCre629. The plasmid was respectively digested by <i>Hin</i>dIII and <i>Bam</i>HI (H+B), split protein NCre (NCre), split protein CCre (CCre), combination of split protein NCre and CCre (NCre + CCre), intact protein Cre (Cre) and MBP. Plasmid and MBP were used as negative control, H+B digestions were used as positive control. MBP tag was used to purify fusion proteins.</p
MOESM2 of Engineered global regulator H-NS improves the acid tolerance of E. coli
Additional file 2: Figure S1. Cell morphology of strains MG, and strains harboring H-NS wild type or mutants
The <i>in vivo</i> recombination of split-Cre protein and the deletions determined by GUS activity.
<p><b>A: Digram of plant expression vectors.</b> pCambia refer to vector of pCambia1305.1. <b>B: GUS staining of transgenic hair roots for each transformant.</b> “n” represents nuclear localization signal. The following are all the same.</p
MOESM1 of Engineered global regulator H-NS improves the acid tolerance of E. coli
Additional file 1: Table S1. Maximum growth rate of strains harboring H-NS mutants under acid stress