21 research outputs found
Planktonic Microbes in the Gulf of Maine Area
In the Gulf of Maine area (GoMA), as elsewhere in the ocean, the organisms of greatest numerical abundance are microbes. Viruses in GoMA are largely cyanophages and bacteriophages, including podoviruses which lack tails. There is also evidence of Mimivirus and Chlorovirus in the metagenome. Bacteria in GoMA comprise the dominant SAR11 phylotype cluster, and other abundant phylotypes such as SAR86-like cluster, SAR116-like cluster, Roseobacter, Rhodospirillaceae, Acidomicrobidae, Flavobacteriales, Cytophaga, and unclassified Alphaproteobacteria and Gammaproteobacteria clusters. Bacterial epibionts of the dinoflagellate Alexandrium fundyense include Rhodobacteraceae, Flavobacteriaceae, Cytophaga spp., Sulfitobacter spp., Sphingomonas spp., and unclassified Bacteroidetes. Phototrophic prokaryotes in GoMA include cyanobacteria that contain chlorophyll (mainly Synechococcus), aerobic anoxygenic phototrophs that contain bacteriochlorophyll, and bacteria that contain proteorhodopsin. Eukaryotic microalgae in GoMA include Bacillariophyceae, Dinophyceae, Prymnesiophyceae, Prasinophyceae, Trebouxiophyceae, Cryptophyceae, Dictyochophyceae, Chrysophyceae, Eustigmatophyceae, Pelagophyceae, Synurophyceae, and Xanthophyceae. There are no records of Bolidophyceae, Aurearenophyceae, Raphidophyceae, and Synchromophyceae in GoMA. In total, there are records for 665 names and 229 genera of microalgae. Heterotrophic eukaryotic protists in GoMA include Dinophyceae, Alveolata, Apicomplexa, amoeboid organisms, Labrynthulida, and heterotrophic marine stramenopiles (MAST). Ciliates include Strombidium, Lohmaniella, Tontonia, Strobilidium, Strombidinopsis and the mixotrophs Laboea strobila and Myrionecta rubrum (ex Mesodinium rubra). An inventory of selected microbial groups in each of 14 physiographic regions in GoMA is made by combining information on the depth-dependent variation of cell density and the depth-dependent variation of water volume. Across the entire GoMA, an estimate for the minimum abundance of cell-based microbes is 1.7Ă1025 organisms. By one account, this number of microbes implies a richness of 105 to 106 taxa in the entire water volume of GoMA. Morphological diversity in microplankton is well-described but the true extent of taxonomic diversity, especially in the femtoplankton, picoplankton and nanoplankton â whether autotrophic, heterotrophic, or mixotrophic, is unknown
Viral to metazoan marine plankton nucleotide sequences from the Tara Oceans expedition
A unique collection of oceanic samples was gathered by the Tara Oceans expeditions (2009â2013), targeting plankton organisms ranging from viruses to metazoans, and providing rich environmental context measurements. Thanks to recent advances in the field of genomics, extensive sequencing has been performed for a deep genomic analysis of this huge collection of samples. A strategy based on different approaches, such as metabarcoding, metagenomics, single-cell genomics and metatranscriptomics, has been chosen for analysis of size-fractionated plankton communities. Here, we provide detailed procedures applied for genomic data generation, from nucleic acids extraction to sequence production, and we describe registries of genomics datasets available at the European Nucleotide Archive (ENA, www.ebi.ac.uk/ena). The association of these metadata to the experimental procedures applied for their generation will help the scientific community to access these data and facilitate their analysis. This paper complements other efforts to provide a full description of experiments and open science resources generated from the Tara Oceans project, further extending their value for the study of the worldâs planktonic ecosystems
Viral to metazoan marine plankton nucleotide sequences from the Tara Oceans expedition
A unique collection of oceanic samples was gathered by the Tara Oceans expeditions (2009-2013), targeting plankton organisms ranging from viruses to metazoans, and providing rich environmental context measurements. Thanks to recent advances in the field of genomics, extensive sequencing has been performed for a deep genomic analysis of this huge collection of samples. A strategy based on different approaches, such as metabarcoding, metagenomics, single-cell genomics and metatranscriptomics, has been chosen for analysis of size-fractionated plankton communities. Here, we provide detailed procedures applied for genomic data generation, from nucleic acids extraction to sequence production, and we describe registries of genomics datasets available at the European Nucleotide Archive (ENA, www.ebi.ac.uk/ena). The association of these metadata to the experimental procedures applied for their generation will help the scientific community to access these data and facilitate their analysis. This paper complements other efforts to provide a full description of experiments and open science resources generated from the Tara Oceans project, further extending their value for the study of the world's planktonic ecosystems
A Spatial Modeling Approach to Predicting the Secondary Spread of Invasive Species Due to Ballast Water Discharge
<div><p>Ballast water in ships is an important contributor to the secondary spread of invasive species in the Laurentian Great Lakes. Here, we use a model previously created to determine the role ballast water management has played in the secondary spread of viral hemorrhagic septicemia virus (VHSV) to identify the future spread of one current and two potential invasive species in the Great Lakes, the Eurasian Ruffe (<i>Gymnocephalus cernuus</i>), killer shrimp (<i>Dikerogammarus villosus</i>), and golden mussel (<i>Limnoperna fortunei</i>), respectively. Model predictions for Eurasian Ruffe have been used to direct surveillance efforts within the Great Lakes and DNA evidence of ruffe presence was recently reported from one of three high risk port localities identified by our model. Predictions made for killer shrimp and golden mussel suggest that these two species have the potential to become rapidly widespread if introduced to the Great Lakes, reinforcing the need for proactive ballast water management. The model used here is flexible enough to be applied to any species capable of being spread by ballast water in marine or freshwater ecosystems.</p></div
Prediction results for the top 25 U.S. ports receiving the most visits by de-ballasting ships.
<p>Numbers represent the number of iterations out of 100 that were predicted to become invaded in the first year modeled. Ports that were outside of the area considered habitable for a species are indicated by NA.</p><p>Prediction results for the top 25 U.S. ports receiving the most visits by de-ballasting ships.</p
Mean number of discharging ship visits per year for each discharge location.
<p>Means between 0 and 1 were rounded up to 1. Ship visit data were obtained for ships visiting U.S. ports between 2004 and 2010 from the National Ballast Information Clearinghouse (Smithsonian Environmental Research Center and USCG 2009).</p
Zebra mussel presences from 1986 to 1992.
<p>Zebra mussel data were obtained from the Nonindigenous Aquatic Species (NAS) database (USGS 2009).</p
Still feeling the effects: A citation analysis of the highly cited, retracted article on MMR vaccines and autism
Killer shrimp prediction results.
<p>The maps illustrate the results of the killer shrimp prediction models with probability of infestation â=â0.50 and no dispersal distance. Invasions were started from Figure 6A Duluth, Minnesota, USA and Figure 6B Toledo, Ohio, USA. The maps depict the next likely invaded locations from current observed presences.</p
Backcasting results for Eurasian Ruffe and zebra mussel.
<p>Graphs AâC illustrate the results for Eurasian Ruffe, and graphs DâE illustrate the results for zebra mussel. Graphs A and D depict the overall accuracy of the models tested. Graphs B and E depict the presence accuracy, or ability to correctly identify presences correctly. Graphs C and F display the absence accuracy, or ability to correctly identify absences correctly. Error bars represent standard deviations.</p