Down-regulation of AR expression inhibits the growth of androgen-sensitive prostate cancer cells

<p><b>Copyright information:</b></p><p>Taken from "A promoting role of androgen receptor in androgen-sensitive and -insensitive prostate cancer cells"</p><p></p><p>Nucleic Acids Research 2007;35(8):2767-2776.</p><p>Published online 10 Apr 2007</p><p>PMCID:PMC1885678.</p><p>© 2007 The Author(s)</p> () LNCaP cells were seeded into 96-well plates in media with or without DHT after 3 h adenovirus infection at an MOI of 10. Cell growth was measured every other day by MTS assay. The data represent the mean ± SD of three independent experiments. () Identical experiments performed in LAPC4 cells. () LNCaP cells were seeded into 24-well plates at 400 cells/well after 3 h adenovirus infection at an MOI of 10. Cells were cultured with the media in the presence or absence of DHT for 14 days and colonies were fixed and stained with crystal violet. () Similar experiments performed in LAPC4 cells

Down-regulation of endogenous AR expression by AR shRNA in prostate cancer cells

<p><b>Copyright information:</b></p><p>Taken from "A promoting role of androgen receptor in androgen-sensitive and -insensitive prostate cancer cells"</p><p></p><p>Nucleic Acids Research 2007;35(8):2767-2776.</p><p>Published online 10 Apr 2007</p><p>PMCID:PMC1885678.</p><p>© 2007 The Author(s)</p> () LNCaP cells were infected with either the GFP adenovirus or the different AR shRNA adenovirus at an MOI of 40. Whole-cell lysates were prepared after 48 h of viral infection, and then analyzed by western blotting. Specific antibodies used to detect protein expression are labeled in the figure. () Identical experiments performed in LAPC4 cells. () LNCaP cells were infected with either the GFP adenovirus or AR shRNA3 adenovirus at an MOI of 40. Cells were fixed and immunostained 72 h after viral infection. Representative confocal laser scanning microscopy images of cells are shown. () Identical experiments performed in LAPC4 cells

Reduction of AR expression inhibits tumor xenograft formation in athymic mice

<p><b>Copyright information:</b></p><p>Taken from "A promoting role of androgen receptor in androgen-sensitive and -insensitive prostate cancer cells"</p><p></p><p>Nucleic Acids Research 2007;35(8):2767-2776.</p><p>Published online 10 Apr 2007</p><p>PMCID:PMC1885678.</p><p>© 2007 The Author(s)</p> () LAPC4 cells were transduced with the AR shRNA or GFP lentiviruses at a MOI of 3 for 24 h. Cells were harvested, resuspended in PBS and mixed with an equal volume of Matrigel ECM. Here, 100 μl of cell suspension (1 × 10 cells/ml) was injected subcutaneously in opposite lateral flanks of 6–8-week-old athymic male mice. Mice were monitored twice weekly. Tumors were measured in two dimensions with calipers, and tumor volume (mm) was calculated with the formula  = (length × width)/2. ‘Asterisk’ indicates a significant difference