Down-regulation of endogenous AR expression by AR shRNA in prostate cancer cells

Abstract

<p><b>Copyright information:</b></p><p>Taken from "A promoting role of androgen receptor in androgen-sensitive and -insensitive prostate cancer cells"</p><p></p><p>Nucleic Acids Research 2007;35(8):2767-2776.</p><p>Published online 10 Apr 2007</p><p>PMCID:PMC1885678.</p><p>© 2007 The Author(s)</p> () LNCaP cells were infected with either the GFP adenovirus or the different AR shRNA adenovirus at an MOI of 40. Whole-cell lysates were prepared after 48 h of viral infection, and then analyzed by western blotting. Specific antibodies used to detect protein expression are labeled in the figure. () Identical experiments performed in LAPC4 cells. () LNCaP cells were infected with either the GFP adenovirus or AR shRNA3 adenovirus at an MOI of 40. Cells were fixed and immunostained 72 h after viral infection. Representative confocal laser scanning microscopy images of cells are shown. () Identical experiments performed in LAPC4 cells