Real-time autonomous remote monitoring of AD

Real-time autonomous remote monitoring of A

Novel monitoring of AD process using low cost sensing platforms

Novel monitoring of AD process using low cost sensing platform

Nõukogude aja ja 1990.-2000. aastate reisikogemused biograafilises perspektiivis

A tar archive containing the HiFive software library. (BZ2 578 kb

Sample datasets for E. coli C-1 genome assembly tutorial

<p>The dataset contains three files:</p> <ol> <li>Illumina_f.fq - forward reads from MiSeq run</li> <li>Illumina_r_fq - reverse reads from MiSeq run</li> <li>minion_2d.fq - Oxford Nanopore 2d reads</li> </ol> <p>Sequencing was done on genomic DNA of E. coli strain C-1 obtained from Yale Stock Center.</p

Fig 8 -

Heatmaps showing expression of oxylipin biosynthetic genes from the C. sativa gene expression atlas (A) or female trichomes of diverse marijuana lines (B). Cells values are rounded TPM values and are colored according to the legend.</p

Working model of <i>C</i>. <i>sativa</i> oxylipin biosynthetic enzyme isoforms and pathways involved in cannabinoid, GLV, and JA biosynthesis.

Abbreviations: [enzymes]: allene oxide cyclase (AOC), allene oxide synthase (AOS), 2-alkynal reductase (2-AR), hydroperoxide lyase (HPL), lipoxygenase (LOX), methylerythritol 4-phosphate pathway (MEP), 12-oxo-phytodienoic reductase (OPR); [metabolites]: (9Z, 11E, 13S, 15Z)-12,13-epoxy-9,11,15-octadecatrienoic acid (12,13-EOT), (9Z, 11E, 13S)-13-hydroperoxy-9,11-octadecadienoic acid (13-HPOD), (9Z, 11E, 13S, 15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid (13-HPOT), cannabigerolic acid (CBGA), linoleic acid (C18:2), α-linolenic acid (C18:3), jasmonic acid (JA), 12-oxo-phytodienoic acid (12-OPDA), 3-oxo-2-(2′[Z]-pentenyl)-cyclopentane-1-octanoic acid (OPC8:0). The gray box indicates uncertainty in subcellular localization.</p

Oxylipin biosynthetic gene collinearity between <i>A</i>. <i>thaliana</i>, <i>C</i>. <i>sativa</i>, and <i>S</i>. <i>lycopersicum</i>.

The grey lines represent collinear genetic blocks between the genomes of A. thaliana and C. sativa and between C. sativa and S. lycospersicum. Bars represent chromosomes and the black, blue, and orange lines represent the collinear pairs of LOX, CYP74, and AOC gene families, respectively.</p

Phylogenetic, genetic, motif, and domain analysis of the <i>C</i>. <i>sativa</i> CYP74 gene family.

(A) Cladogram of peptide sequences and conserved domains. (B) Distribution of conserved peptide sequence motifs. Colors are described in legend; x-axis represents length of peptides in amino acids. (C) Diagram of genetic structure. Blue bars, orange bars, and gray lines represent untranslated regions, exons, and introns, respectively; x-axis represents length of gene in nucleotides. (D) Polar cladogram depicting evolutionary relationship with gene families of selected species. Node labels show confidence values from 1000 bootstrap replications.</p

Gene network analysis.

Cannabis sativa is a global multi-billion-dollar cash crop with numerous industrial uses, including in medicine and recreation where its value is largely owed to the production of pharmacological and psychoactive metabolites known as cannabinoids. Often underappreciated in this role, the lipoxygenase (LOX)-derived green leaf volatiles (GLVs), also known as the scent of cut grass, are the hypothetical origin of hexanoic acid, the initial substrate for cannabinoid biosynthesis. The LOX pathway is best known as the primary source of plant oxylipins, molecules analogous to the eicosanoids from mammalian systems. These molecules are a group of chemically and functionally diverse fatty acid-derived signals that govern nearly all biological processes including plant defense and development. The interaction between oxylipin and cannabinoid biosynthetic pathways remains to be explored. Despite their unique importance in this crop, there has not been a comprehensive investigation focusing on the genes responsible for oxylipin biosynthesis in any Cannabis species. This study documents the first genome-wide catalogue of the Cannabis sativa oxylipin biosynthetic genes and identified 21 LOX, five allene oxide synthases (AOS), three allene oxide cyclases (AOC), one hydroperoxide lyase (HPL), and five 12-oxo-phytodienoic acid reductases (OPR). Gene collinearity analysis found chromosomal regions containing several isoforms maintained across Cannabis, Arabidopsis, and tomato. Promoter, expression, weighted co-expression genetic network, and functional enrichment analysis provide evidence of tissue- and cultivar-specific transcription and roles for distinct isoforms in oxylipin and cannabinoid biosynthesis. This knowledge facilitates future targeted approaches towards Cannabis crop improvement and for the manipulation of cannabinoid metabolism.</div

Expression of oxylipin biosynthetic genes across four developmental stages in <i>C</i>. <i>sativa</i> flowers and trichomes.

Expression of oxylipin biosynthetic genes across four developmental stages in C. sativa flowers and trichomes.</p