1,559 research outputs found
Ribosome heterogeneity and specialization in development
Regulation of protein synthesis is a vital step in controlling gene expression, especially during development. Over the last 10âyears, it has become clear that rather than being homogeneous machines responsible for mRNA translation, ribosomes are highly heterogeneous and can play an active part in translational regulation. These âspecialized ribosomesâ comprise of specific protein and/or rRNA components, which are required for the translation of particular mRNAs. However, while there is extensive evidence for ribosome heterogeneity, support for specialized functions is limited. Recent work in a variety of developmental model organisms has shed some light on the biological relevance of ribosome heterogeneity. Tissueâspecific expression of ribosomal components along with phenotypic analysis of ribosomal gene mutations indicate that ribosome heterogeneity and potentially specialization are common in key development processes like embryogenesis, spermatogenesis, oogenesis, body patterning, and neurogenesis. Several examples of ribosome specialization have now been proposed but strong links between ribosome heterogeneity, translation of specific mRNAs by defined mechanisms, and role of these translation events remain elusive. Furthermore, several studies have indicated that heterogeneous ribosome populations are a product of tissueâspecific expression rather than specialized function and that ribosomal protein phenotypes are the result of extraâribosomal function or overall reduced ribosome levels. Many important questions still need to be addressed in order to determine the functional importance of ribosome heterogeneity to development and disease, which is likely to vary across systems. It will be essential to dissect these issues to fully understand diseases caused by disruptions to ribosomal composition, such as ribosomopathies.
This article is categorized under:
Translation > Translation Regulation
Translation > Ribosome Structure/Function
RNA in Disease and Development > RNA in Developmen
Elastic Wave Diffraction at Cracks in Anisotropic Materials
Ultrasonic inspection is used to confirm that there are no defects of concern in various regions of a nuclear reactor primary circuit. All materials are naturally anisotropic, but if the grains are small relative to the ultrasonic wavelength and are also randomly oriented, then the material will appear as homogeneous and isotropic as in ferritic steel. The ultrasonic wavelength is chosen as a compromise between resolution of defect size and acoustic noise from grain boundaries. In austenitic steel, the wavelength chosen will typically be smaller than the grain size, at least in one direction. The grains are not randomly oriented but exhibit macroscopic patterns which depend on the welding process, and the material is neither homogeneous nor isotropic
Numerical Calculation of Diffraction Coefficients in Anisotropic Media
Ultrasonic inspection is used to detect and size crack-like defects in pressure vessels and pipework used in the nuclear industry. Reliable inspection can only be achieved if the inspection technique is understood, is optimised and subsequently applied correctly. Austenitic steels are used because of their corrosion resistance and toughness. Welds and centrifugally cast materials tend to crystallise with grains larger than the ultrasonic wavelength required to achieve the desired resolution in the inspection and thus appear anisotropic. Since the grains in a weld grow along the, varying, directions of maximum heat flux during cooling, the welds are inhomogeneous as well as anisotropic. We wish to understand the ultrasonic signals scattered by cracks in such inhomogeneous anisotropic materials. To calculate large numbers of cases we would like to use a relatively efficient tool: (ray tracing) and wish to incorporate the diffraction and reflection which occurs at the defect through the use of diffraction or scattering coefficients.</p
Exploring the Universe with Metal-Poor Stars
The early chemical evolution of the Galaxy and the Universe is vital to our
understanding of a host of astrophysical phenomena. Since the most metal-poor
Galactic stars (with metallicities down to [Fe/H]\sim-5.5) are relics from the
high-redshift Universe, they probe the chemical and dynamical conditions of the
Milky Way and the origin and evolution of the elements through nucleosynthesis.
They also provide constraints on the nature of the first stars, their
associated supernovae and initial mass function, and early star and galaxy
formation. The Milky Way's dwarf satellites contain a large fraction (~30%) of
the known most metal-poor stars that have chemical abundances that closely
resemble those of equivalent halo stars. This suggests that chemical evolution
may be universal, at least at early times, and that it is driven by massive,
energetic SNe. Some of these surviving, ultra-faint systems may show the
signature of just one such PopIII star; they may even be surviving first
galaxies. Early analogs of the surviving dwarfs may thus have played an
important role in the assembly of the old Galactic halo whose formation can now
be studied with stellar chemistry. Following the cosmic evolution of small
halos in simulations of structure formation enables tracing the cosmological
origin of the most metal-poor stars in the halo and dwarf galaxies. Together
with future observations and additional modeling, many of these issues,
including the reionization history of the Milky Way, may be constrained this
way. The chapter concludes with an outlook about upcoming observational
challenges and ways forward is to use metal-poor stars to constrain theoretical
studies.Comment: 34 pages, 11 figures. Book chapter to appear in "The First Galaxies -
Theoretical Predictions and Observational Clues", 2012 by Springer, eds. V.
Bromm, B. Mobasher, T. Wiklin
What We Talk about When We Talk about Love: A Duoethnographic Exploration of the Dissertation Relationship
In the aftermath and mop-up following a successful dissertation defense, an unintended and unexpected data source remained unexplored and unanalyzed: 32 audio-recorded discussions and work sessions documenting the processes, approaches, and decisions made by a dissertation director and his doctoral candidate. What might those conversations reveal about the dissertation relationship? Taking a page from Raymond Carverâs short story, âWhat We Talk about When We Talk about Love,â we wondered what we might have been talking about when we were talking about dissertation writing. Inspired and shaped by Norris, Sawyer, and Lundâs (2012. Duoethnography: Dialogic methods for social, health, and educational research. Walnut Creek, CA: Left Coast Press.) duoethnographic methods, this study provides opportunity for us to not just look back on the journey, but pushes us into the messiness of ârecalling and reconceptualizingâ (p. 10). As we each âbecome the foil for the Other, challenging the Other to reflect on their own life in a deeper, more relational, and authentic mannerâ (Norris et al., 2012, p. 10) we also interrogate and trouble our own simplistic categories of analysis
Extragalactic Radio Continuum Surveys and the Transformation of Radio Astronomy
Next-generation radio surveys are about to transform radio astronomy by
discovering and studying tens of millions of previously unknown radio sources.
These surveys will provide new insights to understand the evolution of
galaxies, measuring the evolution of the cosmic star formation rate, and
rivalling traditional techniques in the measurement of fundamental cosmological
parameters. By observing a new volume of observational parameter space, they
are also likely to discover unexpected new phenomena. This review traces the
evolution of extragalactic radio continuum surveys from the earliest days of
radio astronomy to the present, and identifies the challenges that must be
overcome to achieve this transformational change.Comment: To be published in Nature Astronomy 18 Sept 201
Postpartum mental health after Hurricane Katrina: A cohort study
<p>Abstract</p> <p>Background</p> <p>Natural disaster is often a cause of psychopathology, and women are vulnerable to post-traumatic stress disorder (PTSD) and depression. Depression is also common after a woman gives birth. However, no research has addressed postpartum women's mental health after natural disaster.</p> <p>Methods</p> <p>Interviews were conducted in 2006â2007 with women who had been pregnant during or shortly after Hurricane Katrina. 292 New Orleans and Baton Rouge women were interviewed at delivery and 2 months postpartum. Depression was assessed using the Edinburgh Depression Scale and PTSD using the Post-Traumatic Stress Checklist. Women were asked about their experience of the hurricane with questions addressing threat, illness, loss, and damage. Chi-square tests and log-binomial/Poisson models were used to calculate associations and relative risks (RR).</p> <p>Results</p> <p>Black women and women with less education were more likely to have had a serious experience of the hurricane. 18% of the sample met the criteria for depression and 13% for PTSD at two months postpartum. Feeling that one's life was in danger was associated with depression and PTSD, as were injury to a family member and severe impact on property. Overall, two or more severe experiences of the storm was associated with an increased risk for both depression (relative risk (RR) 1.77, 95% confidence interval (CI) 1.08â2.89) and PTSD (RR 3.68, 95% CI 1.80â7.52).</p> <p>Conclusion</p> <p>Postpartum women who experience natural disaster severely are at increased risk for mental health problems, but overall rates of depression and PTSD do not seem to be higher than in studies of the general population.</p
Using small molecules to facilitate exchange of bicarbonate and chloride anions across liposomal membranes
Bicarbonate is involved in a wide range of biological processes, which include respiration, regulation of intracellular pH and fertilization. In this study we use a combination of NMR spectroscopy and ion-selective electrode techniques to show that the natural product prodigiosin, a tripyrrolic molecule produced by microorganisms such as Streptomyces and Serratia, facilitates chloride/bicarbonate exchange (antiport) across liposomal membranes. Higher concentrations of simple synthetic molecules based on a 4,6-dihydroxyisophthalamide core are also shown to facilitate this antiport process. Although it is well known that proteins regulate Cl-/HCO3- exchange in cells, these results suggest that small molecules may also be able to regulate the concentration of these anions in biological systems
Human serum antibodies recognize Treponema denticola Msp and PrtP protease complex proteins
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73677/1/j.1399-302X.2007.00404.x.pd
Natural Disaster and Risk of Psychiatric Disorders in Puerto Rican Children
We examined the persistence of psychiatric disorders at approximately 18 and 30Â months after a hurricane among a random sample of the child and adolescent population (4â17Â years) of Puerto Rico. Data were obtained from caretaker-child dyads (Nâ=â1,886) through in person interviews with primary caretakers (all children) and youth (11â17Â years) using the Diagnostic Interview Schedule for Children IV in Spanish. Logistic regressions, controlling for sociodemographic variables, were used to study the relation between disaster exposure and internalizing, externalizing, or any disorder. Childrenâs disaster-related distress manifested as internalizing disorders, rather than as externalizing disorders at 18Â months post-disaster. At 30Â months, there was no longer a significant difference in rates of disorder between hurricane-exposed and non-exposed youth. Results were similar across age ranges. Rates of specific internalizing disorders between exposed and unexposed children are provided. Research and clinical implications are discussed
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