Night Knights: reminding children that their nighttime fear isn\u27t something they have to face alone

Night Knights are shining toy guardians that children bring to life through play. They engage children’s imagination that inevitably fuels their nighttime fear, and provide a chance to create stories of bravely confronting their fears through their Night Knights

A zinc finger protein array for the visual detection of specific DNA sequences for diagnostic applications.

The visual detection of specific double-stranded DNA sequences possesses great potential for the development of diagnostics. Zinc finger domains provide a powerful scaffold for creating custom DNA-binding proteins that recognize specific DNA sequences. We previously demonstrated sequence-enabled reassembly of TEM-1 β-lactamase (SEER-LAC), a system consisting of two inactive fragments of β-lactamase each linked to engineered zinc finger proteins (ZFPs). Here the SEER-LAC system was applied to develop ZFP arrays that function as simple devices to identify bacterial double-stranded DNA sequences. The ZFP arrays provided a quantitative assay with a detection limit of 50 fmol of target DNA. The method could distinguish target DNA from non-target DNA within 5 min. The ZFP arrays provided sufficient sensitivity and high specificity to recognize specific DNA sequences. These results suggest that ZFP arrays have the potential to be developed into a simple and rapid point-of-care (POC) diagnostic for the multiplexed detection of pathogens

Examining the Different Soil Horizons of White Oak Mountain, TN and their Effect on the Soil Microbial Community

Soil microorganisms participate in the diverse interaction within virtually all ecosystems, consequently affecting the associated human and animal population. Identifying how edaphic variables alter the functional and taxonomic diversity of the soil microbial community requires an examination of total microbial soil diversity and abundance. This research study examined the effect of soil depth and horizon on the soil microbial community composition of White Oak Mountain. The soil microbial community was characterized by 16S/18S/ITS rDNA amplicon sequencing of the DNA extract of six samples from the three major soil types identified: HcE, MoE, and uMvC. OTU clustering analysis and both alpha and beta diversity were analyzed to determine whether soil depth and horizon correlate with soil microbial community composition. This research project aims to establish a baseline of the representative soil microbial diversity of White Oak Mountain and provide data to be used as a reference for future studie

Examining the Different Soil Horizons of White Oak Mountain, TN and their Effect on the Soil Microbial Community

Soil microorganisms participate in the diverse interaction within virtually all ecosystems, consequently affecting the associated human and animal population. Identifying how edaphic variables alter the functional and taxonomic diversity of the soil microbial community requires an examination of total microbial soil diversity and abundance. This research study examined the effect of soil depth and horizon on the soil microbial community composition of White Oak Mountain. The soil microbial community was characterized by 16S/18S/ITS rDNA amplicon sequencing of the DNA extract of six samples from the three major soil types identified: HcE, MoE, and uMvC. OTU clustering analysis and both alpha and beta diversity were analyzed to determine whether soil depth and horizon correlate with soil microbial community composition. This research project aims to establish a baseline of the representative soil microbial diversity of White Oak Mountain and provide data to be used as a reference for future studie