D buzzatii_GENEPOP_data_file

Genotypic data for seven allozyme loci for D. buzzatii flies collected at 67 localities, with a total of 195 samples (collections made more than once at 26 localities). File format - GENEPO

Supplementary Table 1

Identification of the 67 localities (listed north to south), locality code numbers, geographical coordinates and sample size (number of genes assayed

Est fitness crosses

Results of all pairwise crosses - F2 genotype counts and chi square tests for fit to 1:2:1 ratio

IT6_IT15_DATA - Cages 11-20 and associated reperturbation (R) cages

IT6_IT15_DATA - Cages 11-20 and associated reperturbation (R) cage

Mean frequencies of the IT6 (<i>a</i>) allele in the initial populations (excluding cages 13 and 19), mean frequencies of the first reperturbation (R1) populations, of the second (R2-excluding 20R2) and the third (R3), and frequencies in cages 13, 19 and 20R2 at each sampling – IT6/IT15 populations.

<p>Mean frequencies of the IT6 (<i>a</i>) allele in the initial populations (excluding cages 13 and 19), mean frequencies of the first reperturbation (R1) populations, of the second (R2-excluding 20R2) and the third (R3), and frequencies in cages 13, 19 and 20R2 at each sampling – IT6/IT15 populations.</p

All pair-wise crosses, showing those that were not tested (X), those not significant (ns), and results of significance tests for the remaining 23.

<p>Among the latter, f indicates significance for females only and m significance for males only.</p><p>*<i>P</i><0.05,</p><p>**<i>P</i><0.01,</p><p>***<i>P</i><0.001.</p><p>All pair-wise crosses, showing those that were not tested (X), those not significant (ns), and results of significance tests for the remaining 23.</p

Mean frequencies (excluding cages 13 and 19) of the IT6 (<i>a</i>) allele in the initial populations, frequencies in cage 13 and in cage 19, and frequencies in each replicate cage of the first reperturbation (R1), the second (R2) and the third (R3) at each sampling – IT6/IT15 populations.

<p>For clarity, R1 and R2 results are also given separately in the small plots.</p

Frequencies of the IT6 (<i>a</i>) allele in each replicate cage at each sampling of the initial IT6/IH13 populations.

<p>Frequencies of the IT6 (<i>a</i>) allele in each replicate cage at each sampling of the initial IT6/IH13 populations.</p

Selection at the <i>Esterase-2</i> Locus of <i>Drosophila buzzatii</i>? Perturbation-Reperturbation Experiments

<div><p>Apparent selection affecting starch gel electrophoretic alleles at the <i>Esterase-2</i> locus of <i>Drosophila buzzatii</i> has been detected in laboratory and natural populations. Perturbation-reperturbation of allele frequencies in replicated laboratory populations attempts to test direct selective effects at the locus versus effects of linked loci. Sequential gel electrophoresis has identified more alleles within starch classes, and three of these alleles (within the <i>a</i>, <i>b</i> and <i>c</i> starch alleles) were used in cage population experiments. Allele <i>a/1.00/1.00/1.00</i> was set up in 10 replicate populations with allele <i>c/1.00/1.00/1.00</i>, and in an independent 10 replicate populations with allele <i>b/0.99/1.01/1.00</i>. For each set, three reperturbations were done. Replicate populations generally showed similar patterns of allele frequency change and clear directionality: effects of selection, not drift. However, four populations deviated from their replicates, indicating dissipation of linkage disequilibrium. Estimates of pre-adult viability in the F₂ of pair-wise crosses among 12 sequential gel electrophoretic alleles showed very variable modes of inheritance and relative viability fitnesses. Together with the diversity of patterns of allele frequency change in the cage populations, these results suggest a gene complex, with selection acting on an interacting set of loci which may include <i>Esterase</i>-2.</p></div

Frequencies of the IT6 (<i>a</i>) allele in each replicate cage at each sampling of the initial IT6/IT15 populations.

<p>Frequencies of the IT6 (<i>a</i>) allele in each replicate cage at each sampling of the initial IT6/IT15 populations.</p